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High-resolution transcriptional analysis of the regulatory influence of cell-to-cell signalling reveals novel genes that contribute to Xanthomonas phytopathogenesis

High-resolution transcriptional analysis of the regulatory influence of cell-to-cell signalling reveals novel genes that contribute to Xanthomonas phytopathogenesis
High-resolution transcriptional analysis of the regulatory influence of cell-to-cell signalling reveals novel genes that contribute to Xanthomonas phytopathogenesis

The bacterium Xanthomonas campestris is an economically important pathogen of many crop species and a model for the study of bacterial phytopathogenesis. In X.campestris, a regulatory system mediated by the signal molecule DSF controls virulence to plants. The synthesis and recognition of the DSF signal depends upon different Rpf proteins. DSF signal generation requires RpfF whereas signal perception and transduction depends upon a system comprising the sensor RpfC and regulator RpfG. Here we have addressed the action and role of Rpf/DSF signalling in phytopathogenesis by high-resolution transcriptional analysis coupled to functional genomics. We detected transcripts for many genes that were unidentified by previous computational analysis of the genome sequence. Novel transcribed regions included intergenic transcripts predicted as coding or non-coding as well as those that were antisense to coding sequences. In total, mutation of rpfF, rpfG and rpfC led to alteration in transcript levels (more than fourfold) of approximately 480 genes. The regulatory influence of RpfF and RpfC demonstrated considerable overlap. Contrary to expectation, the regulatory influence of RpfC and RpfG had limited overlap, indicating complexities of the Rpf signalling system. Importantly, functional analysis revealed over 160 new virulence factors within the group of Rpf-regulated genes.

0950-382X
1058-1069
An, Shi Qi
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Febrer, Melanie
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Mccarthy, Yvonne
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Tang, Dong Jie
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Clissold, Leah
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Kaithakottil, Gemy
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Swarbreck, David
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Tang, Ji Liang
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Rogers, Jane
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Dow, J. Maxwell
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Ryan, Robert P.
cd9f1e35-9ffe-456f-a64e-798b1f520298
An, Shi Qi
0e05f480-cec1-4c0e-bc1d-359d30ea9a6e
Febrer, Melanie
cdfa7831-01ec-4106-837e-5b345cf4a360
Mccarthy, Yvonne
63ab1257-a428-427a-a560-30d6bd3922e3
Tang, Dong Jie
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Clissold, Leah
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Kaithakottil, Gemy
664a8dc9-e1a1-48b2-906e-e294e777f270
Swarbreck, David
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Tang, Ji Liang
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Rogers, Jane
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Dow, J. Maxwell
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Ryan, Robert P.
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An, Shi Qi, Febrer, Melanie, Mccarthy, Yvonne, Tang, Dong Jie, Clissold, Leah, Kaithakottil, Gemy, Swarbreck, David, Tang, Ji Liang, Rogers, Jane, Dow, J. Maxwell and Ryan, Robert P. (2013) High-resolution transcriptional analysis of the regulatory influence of cell-to-cell signalling reveals novel genes that contribute to Xanthomonas phytopathogenesis. Molecular Microbiology, 88 (6), 1058-1069. (doi:10.1111/mmi.12229).

Record type: Article

Abstract

The bacterium Xanthomonas campestris is an economically important pathogen of many crop species and a model for the study of bacterial phytopathogenesis. In X.campestris, a regulatory system mediated by the signal molecule DSF controls virulence to plants. The synthesis and recognition of the DSF signal depends upon different Rpf proteins. DSF signal generation requires RpfF whereas signal perception and transduction depends upon a system comprising the sensor RpfC and regulator RpfG. Here we have addressed the action and role of Rpf/DSF signalling in phytopathogenesis by high-resolution transcriptional analysis coupled to functional genomics. We detected transcripts for many genes that were unidentified by previous computational analysis of the genome sequence. Novel transcribed regions included intergenic transcripts predicted as coding or non-coding as well as those that were antisense to coding sequences. In total, mutation of rpfF, rpfG and rpfC led to alteration in transcript levels (more than fourfold) of approximately 480 genes. The regulatory influence of RpfF and RpfC demonstrated considerable overlap. Contrary to expectation, the regulatory influence of RpfC and RpfG had limited overlap, indicating complexities of the Rpf signalling system. Importantly, functional analysis revealed over 160 new virulence factors within the group of Rpf-regulated genes.

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An_et_al-2013-Molecular_Microbiology - Version of Record
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Published date: June 2013

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Local EPrints ID: 425815
URI: https://eprints.soton.ac.uk/id/eprint/425815
ISSN: 0950-382X
PURE UUID: a3b583ee-22bf-4b62-aa54-ed1091047cda

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Date deposited: 05 Nov 2018 17:30
Last modified: 13 Mar 2019 17:52

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Contributors

Author: Shi Qi An
Author: Melanie Febrer
Author: Yvonne Mccarthy
Author: Dong Jie Tang
Author: Leah Clissold
Author: Gemy Kaithakottil
Author: David Swarbreck
Author: Ji Liang Tang
Author: Jane Rogers
Author: J. Maxwell Dow
Author: Robert P. Ryan

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