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A cyclic GMP-dependent signalling pathway regulates bacterial phytopathogenesis

A cyclic GMP-dependent signalling pathway regulates bacterial phytopathogenesis
A cyclic GMP-dependent signalling pathway regulates bacterial phytopathogenesis

Cyclic guanosine 3',5'-monophosphate (cyclic GMP) is a second messenger whose role in bacterial signalling is poorly understood. A genetic screen in the plant pathogen Xanthomonas campestris (Xcc) identified that XC-0250, which encodes a protein with a class III nucleotidyl cyclase domain, is required for cyclic GMP synthesis. Purified XC-0250 was active in cyclic GMP synthesis in vitro. The linked gene XC-0249 encodes a protein with a cyclic mononucleotide-binding (cNMP) domain and a GGDEF diguanylate cyclase domain. The activity of XC-0249 in cyclic di-GMP synthesis was enhanced by addition of cyclic GMP. The isolated cNMP domain of XC-0249 bound cyclic GMP and a structure-function analysis, directed by determination of the crystal structure of the holo-complex, demonstrated the site of cyclic GMP binding that modulates cyclic di-GMP synthesis. Mutation of either XC-0250 or XC-0249 led to a reduced virulence to plants and reduced biofilm formation in vitro. These findings describe a regulatory pathway in which cyclic GMP regulates virulence and biofilm formation through interaction with a novel effector that directly links cyclic GMP and cyclic di-GMP signalling.

Biofilm, Cyclic di-GMP, Signal transduction, Virulence, Xanthomonas campestris
0261-4189
2430-2438
An, Shi Qi
0e05f480-cec1-4c0e-bc1d-359d30ea9a6e
Chin, Ko Hsin
f6912149-b9cb-4249-9296-257ce77b16d4
Febrer, Melanie
cdfa7831-01ec-4106-837e-5b345cf4a360
McCarthy, Yvonne
63ab1257-a428-427a-a560-30d6bd3922e3
Yang, Jauo Guey
bb087175-3b3a-4a36-819e-ef3963d4c2a7
Liu, Chung Liang
769ceb29-6ec1-4133-89ad-4e030e834cf8
Swarbreck, David
b9fca4d2-48fc-4593-a002-6fc101146be6
Rogers, Jane
32663a03-d618-4f2c-a20e-5f9f44564dcd
Maxwell Dow, J.
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Chou, Shan Ho
df90f6fe-5a0b-48ed-8fec-16cbf4ec4db7
Ryan, Robert P.
cd9f1e35-9ffe-456f-a64e-798b1f520298
An, Shi Qi
0e05f480-cec1-4c0e-bc1d-359d30ea9a6e
Chin, Ko Hsin
f6912149-b9cb-4249-9296-257ce77b16d4
Febrer, Melanie
cdfa7831-01ec-4106-837e-5b345cf4a360
McCarthy, Yvonne
63ab1257-a428-427a-a560-30d6bd3922e3
Yang, Jauo Guey
bb087175-3b3a-4a36-819e-ef3963d4c2a7
Liu, Chung Liang
769ceb29-6ec1-4133-89ad-4e030e834cf8
Swarbreck, David
b9fca4d2-48fc-4593-a002-6fc101146be6
Rogers, Jane
32663a03-d618-4f2c-a20e-5f9f44564dcd
Maxwell Dow, J.
131815f4-de2d-4b5f-abd9-e1bfc3ce7bf3
Chou, Shan Ho
df90f6fe-5a0b-48ed-8fec-16cbf4ec4db7
Ryan, Robert P.
cd9f1e35-9ffe-456f-a64e-798b1f520298

An, Shi Qi, Chin, Ko Hsin, Febrer, Melanie, McCarthy, Yvonne, Yang, Jauo Guey, Liu, Chung Liang, Swarbreck, David, Rogers, Jane, Maxwell Dow, J., Chou, Shan Ho and Ryan, Robert P. (2013) A cyclic GMP-dependent signalling pathway regulates bacterial phytopathogenesis. The EMBO Journal, 32 (18), 2430-2438. (doi:10.1038/emboj.2013.165).

Record type: Article

Abstract

Cyclic guanosine 3',5'-monophosphate (cyclic GMP) is a second messenger whose role in bacterial signalling is poorly understood. A genetic screen in the plant pathogen Xanthomonas campestris (Xcc) identified that XC-0250, which encodes a protein with a class III nucleotidyl cyclase domain, is required for cyclic GMP synthesis. Purified XC-0250 was active in cyclic GMP synthesis in vitro. The linked gene XC-0249 encodes a protein with a cyclic mononucleotide-binding (cNMP) domain and a GGDEF diguanylate cyclase domain. The activity of XC-0249 in cyclic di-GMP synthesis was enhanced by addition of cyclic GMP. The isolated cNMP domain of XC-0249 bound cyclic GMP and a structure-function analysis, directed by determination of the crystal structure of the holo-complex, demonstrated the site of cyclic GMP binding that modulates cyclic di-GMP synthesis. Mutation of either XC-0250 or XC-0249 led to a reduced virulence to plants and reduced biofilm formation in vitro. These findings describe a regulatory pathway in which cyclic GMP regulates virulence and biofilm formation through interaction with a novel effector that directly links cyclic GMP and cyclic di-GMP signalling.

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More information

Published date: 11 September 2013
Keywords: Biofilm, Cyclic di-GMP, Signal transduction, Virulence, Xanthomonas campestris

Identifiers

Local EPrints ID: 425817
URI: https://eprints.soton.ac.uk/id/eprint/425817
ISSN: 0261-4189
PURE UUID: f7b82b01-3345-42ae-b6f7-4204e29137cb

Catalogue record

Date deposited: 05 Nov 2018 17:30
Last modified: 13 Mar 2019 17:52

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Contributors

Author: Shi Qi An
Author: Ko Hsin Chin
Author: Melanie Febrer
Author: Yvonne McCarthy
Author: Jauo Guey Yang
Author: Chung Liang Liu
Author: David Swarbreck
Author: Jane Rogers
Author: J. Maxwell Dow
Author: Shan Ho Chou
Author: Robert P. Ryan

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