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The PAS domain-containing histidine kinase RpfS is a second sensor for the diffusible signal factor of Xanthomonas campestris

The PAS domain-containing histidine kinase RpfS is a second sensor for the diffusible signal factor of Xanthomonas campestris
The PAS domain-containing histidine kinase RpfS is a second sensor for the diffusible signal factor of Xanthomonas campestris

Summary: A cell-cell signalling system mediated by the fatty acid signal DSF controls the virulence of Xanthomonas campestris pv. campestris (Xcc) to plants. The synthesis and recognition of the DSF signal depends upon different Rpf proteins. DSF signal generation requires RpfF whereas signal perception and transduction depends upon the sensor RpfC and regulator RpfG. Detailed analyses of the regulatory roles of different Rpf proteins have suggested the occurrence of further sensors for DSF. Here we have used a mutagenesis approach coupled with high-resolution transcriptional analysis to identify XC_2579 (RpfS) as a second sensor for DSF in Xcc. RpfS is a complex sensor kinase predicted to have multiple Per/Arnt/Sim (PAS) domains, a histidine kinase domain and a C-terminal receiver (REC) domain. Isothermal calorimetry showed that DSF bound to the isolated N-terminal PAS domain with a Kd of 1.4μM. RpfS controlled expression of a sub-set of genes distinct from those controlled by RpfC to include genes involved in type IV secretion and chemotaxis. Mutation of XC_2579 was associated with a reduction in virulence of Xcc to Chinese Radish when assayed by leaf spraying but not by leaf inoculation, suggesting a role for RpfS-controlled factors in the epiphytic phase of the disease cycle.

0950-382X
586-597
An, Shi Qi
0e05f480-cec1-4c0e-bc1d-359d30ea9a6e
Allan, John H.
661c0f3b-2385-442e-8507-972db5ab1255
Mccarthy, Yvonne
63ab1257-a428-427a-a560-30d6bd3922e3
Febrer, Melanie
cdfa7831-01ec-4106-837e-5b345cf4a360
Dow, J. Maxwell
a904f493-80b4-4868-999f-af843fff1063
Ryan, Robert P.
cd9f1e35-9ffe-456f-a64e-798b1f520298
An, Shi Qi
0e05f480-cec1-4c0e-bc1d-359d30ea9a6e
Allan, John H.
661c0f3b-2385-442e-8507-972db5ab1255
Mccarthy, Yvonne
63ab1257-a428-427a-a560-30d6bd3922e3
Febrer, Melanie
cdfa7831-01ec-4106-837e-5b345cf4a360
Dow, J. Maxwell
a904f493-80b4-4868-999f-af843fff1063
Ryan, Robert P.
cd9f1e35-9ffe-456f-a64e-798b1f520298

An, Shi Qi, Allan, John H., Mccarthy, Yvonne, Febrer, Melanie, Dow, J. Maxwell and Ryan, Robert P. (2014) The PAS domain-containing histidine kinase RpfS is a second sensor for the diffusible signal factor of Xanthomonas campestris. Molecular Microbiology, 92 (3), 586-597. (doi:10.1111/mmi.12577).

Record type: Article

Abstract

Summary: A cell-cell signalling system mediated by the fatty acid signal DSF controls the virulence of Xanthomonas campestris pv. campestris (Xcc) to plants. The synthesis and recognition of the DSF signal depends upon different Rpf proteins. DSF signal generation requires RpfF whereas signal perception and transduction depends upon the sensor RpfC and regulator RpfG. Detailed analyses of the regulatory roles of different Rpf proteins have suggested the occurrence of further sensors for DSF. Here we have used a mutagenesis approach coupled with high-resolution transcriptional analysis to identify XC_2579 (RpfS) as a second sensor for DSF in Xcc. RpfS is a complex sensor kinase predicted to have multiple Per/Arnt/Sim (PAS) domains, a histidine kinase domain and a C-terminal receiver (REC) domain. Isothermal calorimetry showed that DSF bound to the isolated N-terminal PAS domain with a Kd of 1.4μM. RpfS controlled expression of a sub-set of genes distinct from those controlled by RpfC to include genes involved in type IV secretion and chemotaxis. Mutation of XC_2579 was associated with a reduction in virulence of Xcc to Chinese Radish when assayed by leaf spraying but not by leaf inoculation, suggesting a role for RpfS-controlled factors in the epiphytic phase of the disease cycle.

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More information

Accepted/In Press date: 10 March 2014
e-pub ahead of print date: 12 March 2014
Published date: May 2014

Identifiers

Local EPrints ID: 425820
URI: http://eprints.soton.ac.uk/id/eprint/425820
ISSN: 0950-382X
PURE UUID: ed2833cf-1ce8-4747-899a-0ee6f876338e

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Date deposited: 05 Nov 2018 17:30
Last modified: 05 Jun 2024 18:50

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Contributors

Author: Shi Qi An
Author: John H. Allan
Author: Yvonne Mccarthy
Author: Melanie Febrer
Author: J. Maxwell Dow
Author: Robert P. Ryan

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