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Probing the role of cyclic di-GMP signaling systems in disease using Chinese radish

Probing the role of cyclic di-GMP signaling systems in disease using Chinese radish
Probing the role of cyclic di-GMP signaling systems in disease using Chinese radish

The determination of the genome sequences of pathogenic bacteria has facilitated functional analyses that aim to understand the molecular basis of virulence. In particular, genome sequence information of the pathogen Xanthomonas campestris pathovar campestris has allowed researchers to identify and functionally analyze the role of intracellular signaling involving cyclic di-GMP in black rot disease of crucifers. Here, we describe leaf clipping and spraying methods for testing the virulence of wild type and derived mutants of X. campestris in Chinese radish. These methods address different facets of the disease cycle, which requires the ability to survive epiphytically before entry into the plant and growth and systemic spread within the xylem.

Leaf clipping, Phytopathogenesis, Spray inoculation, Virulence testing, Xanthomonas campestris
1064-3745
205-212
Humana Press Inc.
An, Shi Qi
0e05f480-cec1-4c0e-bc1d-359d30ea9a6e
Tang, Ji Liang
74e46e1a-4f75-4c94-871d-317339f309f2
Dow, J. Maxwell
a5cbfb71-a37a-4aec-9647-6532e45da084
Sauer, K.
An, Shi Qi
0e05f480-cec1-4c0e-bc1d-359d30ea9a6e
Tang, Ji Liang
74e46e1a-4f75-4c94-871d-317339f309f2
Dow, J. Maxwell
a5cbfb71-a37a-4aec-9647-6532e45da084
Sauer, K.

An, Shi Qi, Tang, Ji Liang and Dow, J. Maxwell (2017) Probing the role of cyclic di-GMP signaling systems in disease using Chinese radish. In, Sauer, K. (ed.) c-di-GMP Signaling. (Methods in Molecular Biology, , (doi:10.1007/978-1-4939-7240-1_16), 1657) New York, NY. Humana Press Inc., pp. 205-212. (doi:10.1007/978-1-4939-7240-1_16).

Record type: Book Section

Abstract

The determination of the genome sequences of pathogenic bacteria has facilitated functional analyses that aim to understand the molecular basis of virulence. In particular, genome sequence information of the pathogen Xanthomonas campestris pathovar campestris has allowed researchers to identify and functionally analyze the role of intracellular signaling involving cyclic di-GMP in black rot disease of crucifers. Here, we describe leaf clipping and spraying methods for testing the virulence of wild type and derived mutants of X. campestris in Chinese radish. These methods address different facets of the disease cycle, which requires the ability to survive epiphytically before entry into the plant and growth and systemic spread within the xylem.

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More information

e-pub ahead of print date: 10 September 2017
Published date: 2017
Keywords: Leaf clipping, Phytopathogenesis, Spray inoculation, Virulence testing, Xanthomonas campestris

Identifiers

Local EPrints ID: 425829
URI: http://eprints.soton.ac.uk/id/eprint/425829
ISSN: 1064-3745
PURE UUID: 2e784db9-1d07-42f9-8a8b-e0d2ac594428

Catalogue record

Date deposited: 05 Nov 2018 17:30
Last modified: 08 Jan 2019 17:30

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