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Diffusible signal factor signaling regulates multiple functions in the opportunistic pathogen Stenotrophomonas maltophilia

Diffusible signal factor signaling regulates multiple functions in the opportunistic pathogen Stenotrophomonas maltophilia
Diffusible signal factor signaling regulates multiple functions in the opportunistic pathogen Stenotrophomonas maltophilia

Objective: Stenotrophomonas maltophilia is a Gram-negative bacterium commonly isolated from nosocomial infections. Analysis of the genome of the clinical S. maltophilia isolate K279a indicates that it encodes a diffusible signal factor (DSF)-dependent cell-cell signaling mechanism that is highly similar to the system previously described in phytopathogens from the genera Xanthomonas and Xylella. Our objective was to study the function of DSF signaling in the clinical strain S. maltophilia K279a using genetic and functional genomic analyses. Results: We compared the wild-type strain with a mutant deficient in the rpfF (regulation of pathogenicity factors) gene that is essential for the synthesis of DSF. The effects of disruption of DSF signaling were pleiotropic with an impact on virulence, biofilm formation and pathogenesis. The phenotypic effects of rpfF mutation in S. maltophilia could be reversed by addition of exogenous DSF. Taken together, we demonstrate that DSF signaling regulates factors contributing to virulence, biofilm formation and motility of this important opportunistic pathogen.

Antibiotic resistance, Biofilm formation, Cell-cell signaling, Diffusible signal factor, Stenotrophomonas maltophilia, Virulence
1756-0500
An, Shi Qi
0e05f480-cec1-4c0e-bc1d-359d30ea9a6e
Tang, Ji Liang
74e46e1a-4f75-4c94-871d-317339f309f2
An, Shi Qi
0e05f480-cec1-4c0e-bc1d-359d30ea9a6e
Tang, Ji Liang
74e46e1a-4f75-4c94-871d-317339f309f2

An, Shi Qi and Tang, Ji Liang (2018) Diffusible signal factor signaling regulates multiple functions in the opportunistic pathogen Stenotrophomonas maltophilia. BMC Research Notes, 11 (1). (doi:10.1186/s13104-018-3690-1).

Record type: Article

Abstract

Objective: Stenotrophomonas maltophilia is a Gram-negative bacterium commonly isolated from nosocomial infections. Analysis of the genome of the clinical S. maltophilia isolate K279a indicates that it encodes a diffusible signal factor (DSF)-dependent cell-cell signaling mechanism that is highly similar to the system previously described in phytopathogens from the genera Xanthomonas and Xylella. Our objective was to study the function of DSF signaling in the clinical strain S. maltophilia K279a using genetic and functional genomic analyses. Results: We compared the wild-type strain with a mutant deficient in the rpfF (regulation of pathogenicity factors) gene that is essential for the synthesis of DSF. The effects of disruption of DSF signaling were pleiotropic with an impact on virulence, biofilm formation and pathogenesis. The phenotypic effects of rpfF mutation in S. maltophilia could be reversed by addition of exogenous DSF. Taken together, we demonstrate that DSF signaling regulates factors contributing to virulence, biofilm formation and motility of this important opportunistic pathogen.

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s13104-018-3690-1 - Version of Record
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Accepted/In Press date: 6 August 2018
e-pub ahead of print date: 10 August 2018
Published date: 10 August 2018
Keywords: Antibiotic resistance, Biofilm formation, Cell-cell signaling, Diffusible signal factor, Stenotrophomonas maltophilia, Virulence

Identifiers

Local EPrints ID: 425831
URI: https://eprints.soton.ac.uk/id/eprint/425831
ISSN: 1756-0500
PURE UUID: f38f5c20-1f97-477a-930d-87ed2358d5cb

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Date deposited: 05 Nov 2018 17:30
Last modified: 13 Mar 2019 17:52

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Contributors

Author: Shi Qi An
Author: Ji Liang Tang

University divisions

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