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The Ax21 protein influences virulence and biofilm formation in Stenotrophomonas maltophilia

The Ax21 protein influences virulence and biofilm formation in Stenotrophomonas maltophilia
The Ax21 protein influences virulence and biofilm formation in Stenotrophomonas maltophilia

Stenotrophomonas maltophilia is an antibiotic-resistant Gram-negative pathogen, which is associated with hospital-acquired infection. The genome encodes a protein highly related to the Ax21 protein of Xanthomonas oryzae that is implicated in interactions of this plant pathogen with rice. Here, we report on the pleiotropic nature of ax21 mutation in S. maltophilia and the effects of addition of the Ax21 protein on the restoration of the wild-type phenotype. We show that loss by mutation of Ax21 leads to reduced motility, reduced biofilm formation, reduced tolerance to the antibiotic tobramycin and reduced virulence to larvae of Galleria mellonella, as well as alteration in the expression of specific genes associated with virulence or antibiotic resistance. Addition of the Ax21protein restored motility and the level of gene expression towards wild type. These findings are consistent with the notion that the Ax21 protein is involved in intraspecies communication, although other interpretations cannot be discounted.

Antibiotic resistance, Biofilm formation, Motility, Stenotrophomonas, Virulence
0302-8933
183-187
An, Shi qi
0e05f480-cec1-4c0e-bc1d-359d30ea9a6e
Tang, Ji liang
74e46e1a-4f75-4c94-871d-317339f309f2
An, Shi qi
0e05f480-cec1-4c0e-bc1d-359d30ea9a6e
Tang, Ji liang
74e46e1a-4f75-4c94-871d-317339f309f2

An, Shi qi and Tang, Ji liang (2018) The Ax21 protein influences virulence and biofilm formation in Stenotrophomonas maltophilia. Archives of Microbiology, 200 (1), 183-187. (doi:10.1007/s00203-017-1433-7).

Record type: Article

Abstract

Stenotrophomonas maltophilia is an antibiotic-resistant Gram-negative pathogen, which is associated with hospital-acquired infection. The genome encodes a protein highly related to the Ax21 protein of Xanthomonas oryzae that is implicated in interactions of this plant pathogen with rice. Here, we report on the pleiotropic nature of ax21 mutation in S. maltophilia and the effects of addition of the Ax21 protein on the restoration of the wild-type phenotype. We show that loss by mutation of Ax21 leads to reduced motility, reduced biofilm formation, reduced tolerance to the antibiotic tobramycin and reduced virulence to larvae of Galleria mellonella, as well as alteration in the expression of specific genes associated with virulence or antibiotic resistance. Addition of the Ax21protein restored motility and the level of gene expression towards wild type. These findings are consistent with the notion that the Ax21 protein is involved in intraspecies communication, although other interpretations cannot be discounted.

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Accepted/In Press date: 19 September 2017
e-pub ahead of print date: 30 September 2017
Published date: 1 January 2018
Keywords: Antibiotic resistance, Biofilm formation, Motility, Stenotrophomonas, Virulence

Identifiers

Local EPrints ID: 425832
URI: https://eprints.soton.ac.uk/id/eprint/425832
ISSN: 0302-8933
PURE UUID: 8f85ad1f-e26f-4869-b17e-8b714f702472

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Date deposited: 05 Nov 2018 17:30
Last modified: 13 Mar 2019 17:52

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