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Immunological methods to study monoclonal antibody activity

Immunological methods to study monoclonal antibody activity
Immunological methods to study monoclonal antibody activity
Over recent decades it has become increasingly apparent that malignant cells, including chronic lymphocytic leukemia (CLL) cells, do not exist in isolation. Rather they coalesce with numerous “normal” cells of the body and, in the case of CLL, inhabit key immunological niches within secondary lymphoid organs (SLO), where a plethora of stromal and immune cells mediate their growth and survival. With the advent and approval of targeted immune therapies such as monoclonal antibodies (mAb), which elicit their efficacy by engaging immune-mediated effector mechanisms, it is important to develop accurate methods to measure their activities. Here, we describe a series of reliable assays capable of measuring important antibody-mediated effector functions: antibody-dependent cellular phagocytosis (ADCP), antibody-dependent cellular cytotoxicity (ADCC), and complement-dependent cytotoxicity (CDC) that measure these immune activities.
173-184
Humana Press
Carter, Matthew
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Dahal, Lekh
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Cleary, Kirstie
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Marshall, Michael
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French, Ruth
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Beers, Stephen
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Cragg, Mark
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Malek, S.
Carter, Matthew
3baac102-d80c-42ba-ab4d-ad339a48169e
Dahal, Lekh
1e993a7a-b007-4187-82ea-e28dd3920b66
Cleary, Kirstie
16e11432-9855-4b5b-8c3f-be86f0ef51f0
Marshall, Michael
ae47eb68-f597-4c49-aced-5e09c4fd1e0c
French, Ruth
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Beers, Stephen
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Cragg, Mark
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Malek, S.

Carter, Matthew, Dahal, Lekh, Cleary, Kirstie, Marshall, Michael, French, Ruth, Beers, Stephen and Cragg, Mark (2019) Immunological methods to study monoclonal antibody activity. In, Malek, S. (ed.) Chronic Lymphocytic Leukaemia. (Methods in Molecular Biology, , (doi:10.1007/978-1-4939-8876-1_14), 1881) New York, NY. Humana Press, pp. 173-184. (doi:10.1007/978-1-4939-8876-1_14).

Record type: Book Section

Abstract

Over recent decades it has become increasingly apparent that malignant cells, including chronic lymphocytic leukemia (CLL) cells, do not exist in isolation. Rather they coalesce with numerous “normal” cells of the body and, in the case of CLL, inhabit key immunological niches within secondary lymphoid organs (SLO), where a plethora of stromal and immune cells mediate their growth and survival. With the advent and approval of targeted immune therapies such as monoclonal antibodies (mAb), which elicit their efficacy by engaging immune-mediated effector mechanisms, it is important to develop accurate methods to measure their activities. Here, we describe a series of reliable assays capable of measuring important antibody-mediated effector functions: antibody-dependent cellular phagocytosis (ADCP), antibody-dependent cellular cytotoxicity (ADCC), and complement-dependent cytotoxicity (CDC) that measure these immune activities.

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Immunological Methods to Study CLL Text and Figures - Accepted Manuscript
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e-pub ahead of print date: 23 October 2018
Published date: 2019

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Local EPrints ID: 425858
URI: https://eprints.soton.ac.uk/id/eprint/425858
PURE UUID: 24144a58-b62b-4683-8dd5-63dd4f565b4e
ORCID for Kirstie Cleary: ORCID iD orcid.org/0000-0001-6200-4945
ORCID for Mark Cragg: ORCID iD orcid.org/0000-0003-2077-089X

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Date deposited: 05 Nov 2018 17:30
Last modified: 14 Mar 2019 01:49

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Contributors

Author: Matthew Carter
Author: Lekh Dahal
Author: Kirstie Cleary ORCID iD
Author: Michael Marshall
Author: Ruth French
Author: Stephen Beers
Author: Mark Cragg ORCID iD
Editor: S. Malek

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