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Ex-vivo signal transduction studies in chronic lymphocytic leukemia

Ex-vivo signal transduction studies in chronic lymphocytic leukemia
Ex-vivo signal transduction studies in chronic lymphocytic leukemia

Microenvironmental signaling is pivotal to chronic lymphocytic leukemia (CLL) pathology; therefore understanding how to investigate this pathway by both protein and chemical methods is crucial if we are to investigate and correlate biological changes with therapeutic responses in patients. Herein, we describe the use of western blotting also referred to as immunoblotting as a method that can semiquantitatively evaluate changes in protein expression following receptor engagement; this includes B cell receptor (BCR) signaling following stimulation with anti-IgM (Blunt et al. Clin Cancer Res 23(9):2313–2324, 2017). It is important to note that immunoblotting should always be combined with other quantitative methods such as flow cytometry to confirm activation of these signaling pathways (Aguilar-Hernandez et al. Blood 127(24):3015–3025, 2016).

Antibodies, B cell receptor signaling, B-cell receptor, Bidirectional electrophoresis, Calcium flux, Flow cytometry, Immunoblot, Immunoblotting, Nitrocellulose membrane, Wet transfer
1064-3745
1-17
Humana Press Inc.
Rogers-Broadway, Karly Rai
5b9a8d13-1f70-40a3-8e30-741a8ad19068
Karydis, Laura I.
f861fc5a-babb-4b1e-a34e-903d226ea38f
Dobson, Rachel C.
c2ef3195-3497-4d01-b75e-e0f2dd668ec3
Steele, Andrew J.
4349f6aa-2e3a-49a8-be73-7716056ae089
Malek, S.
Rogers-Broadway, Karly Rai
5b9a8d13-1f70-40a3-8e30-741a8ad19068
Karydis, Laura I.
f861fc5a-babb-4b1e-a34e-903d226ea38f
Dobson, Rachel C.
c2ef3195-3497-4d01-b75e-e0f2dd668ec3
Steele, Andrew J.
4349f6aa-2e3a-49a8-be73-7716056ae089
Malek, S.

Rogers-Broadway, Karly Rai, Karydis, Laura I., Dobson, Rachel C. and Steele, Andrew J. (2019) Ex-vivo signal transduction studies in chronic lymphocytic leukemia. In, Malek, S. (ed.) Chronic Lymphocytic Leukemia. (Methods in Molecular Biology, 1881) New York. Humana Press Inc., pp. 1-17. (doi:10.1007/978-1-4939-8876-1_1).

Record type: Book Section

Abstract

Microenvironmental signaling is pivotal to chronic lymphocytic leukemia (CLL) pathology; therefore understanding how to investigate this pathway by both protein and chemical methods is crucial if we are to investigate and correlate biological changes with therapeutic responses in patients. Herein, we describe the use of western blotting also referred to as immunoblotting as a method that can semiquantitatively evaluate changes in protein expression following receptor engagement; this includes B cell receptor (BCR) signaling following stimulation with anti-IgM (Blunt et al. Clin Cancer Res 23(9):2313–2324, 2017). It is important to note that immunoblotting should always be combined with other quantitative methods such as flow cytometry to confirm activation of these signaling pathways (Aguilar-Hernandez et al. Blood 127(24):3015–3025, 2016).

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More information

e-pub ahead of print date: 23 October 2018
Published date: 2019
Keywords: Antibodies, B cell receptor signaling, B-cell receptor, Bidirectional electrophoresis, Calcium flux, Flow cytometry, Immunoblot, Immunoblotting, Nitrocellulose membrane, Wet transfer

Identifiers

Local EPrints ID: 425937
URI: http://eprints.soton.ac.uk/id/eprint/425937
ISSN: 1064-3745
PURE UUID: 3785f942-1763-4566-9e40-be374707df4d
ORCID for Andrew J. Steele: ORCID iD orcid.org/0000-0003-0667-1596

Catalogue record

Date deposited: 07 Nov 2018 17:30
Last modified: 10 Nov 2021 03:28

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Contributors

Author: Karly Rai Rogers-Broadway
Author: Laura I. Karydis
Author: Rachel C. Dobson
Editor: S. Malek

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