Ex-vivo signal transduction studies in chronic lymphocytic leukemia
Ex-vivo signal transduction studies in chronic lymphocytic leukemia
Microenvironmental signaling is pivotal to chronic lymphocytic leukemia (CLL) pathology; therefore understanding how to investigate this pathway by both protein and chemical methods is crucial if we are to investigate and correlate biological changes with therapeutic responses in patients. Herein, we describe the use of western blotting also referred to as immunoblotting as a method that can semiquantitatively evaluate changes in protein expression following receptor engagement; this includes B cell receptor (BCR) signaling following stimulation with anti-IgM (Blunt et al. Clin Cancer Res 23(9):2313–2324, 2017). It is important to note that immunoblotting should always be combined with other quantitative methods such as flow cytometry to confirm activation of these signaling pathways (Aguilar-Hernandez et al. Blood 127(24):3015–3025, 2016).
Antibodies, B cell receptor signaling, B-cell receptor, Bidirectional electrophoresis, Calcium flux, Flow cytometry, Immunoblot, Immunoblotting, Nitrocellulose membrane, Wet transfer
1-17
Rogers-Broadway, Karly Rai
5b9a8d13-1f70-40a3-8e30-741a8ad19068
Karydis, Laura I.
f861fc5a-babb-4b1e-a34e-903d226ea38f
Dobson, Rachel C.
c2ef3195-3497-4d01-b75e-e0f2dd668ec3
Steele, Andrew J.
4349f6aa-2e3a-49a8-be73-7716056ae089
2019
Rogers-Broadway, Karly Rai
5b9a8d13-1f70-40a3-8e30-741a8ad19068
Karydis, Laura I.
f861fc5a-babb-4b1e-a34e-903d226ea38f
Dobson, Rachel C.
c2ef3195-3497-4d01-b75e-e0f2dd668ec3
Steele, Andrew J.
4349f6aa-2e3a-49a8-be73-7716056ae089
Rogers-Broadway, Karly Rai, Karydis, Laura I., Dobson, Rachel C. and Steele, Andrew J.
(2019)
Ex-vivo signal transduction studies in chronic lymphocytic leukemia.
In,
Malek, S.
(ed.)
Chronic Lymphocytic Leukemia.
(Methods in Molecular Biology, 1881)
New York.
Humana New York, .
(doi:10.1007/978-1-4939-8876-1_1).
Record type:
Book Section
Abstract
Microenvironmental signaling is pivotal to chronic lymphocytic leukemia (CLL) pathology; therefore understanding how to investigate this pathway by both protein and chemical methods is crucial if we are to investigate and correlate biological changes with therapeutic responses in patients. Herein, we describe the use of western blotting also referred to as immunoblotting as a method that can semiquantitatively evaluate changes in protein expression following receptor engagement; this includes B cell receptor (BCR) signaling following stimulation with anti-IgM (Blunt et al. Clin Cancer Res 23(9):2313–2324, 2017). It is important to note that immunoblotting should always be combined with other quantitative methods such as flow cytometry to confirm activation of these signaling pathways (Aguilar-Hernandez et al. Blood 127(24):3015–3025, 2016).
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More information
e-pub ahead of print date: 23 October 2018
Published date: 2019
Keywords:
Antibodies, B cell receptor signaling, B-cell receptor, Bidirectional electrophoresis, Calcium flux, Flow cytometry, Immunoblot, Immunoblotting, Nitrocellulose membrane, Wet transfer
Identifiers
Local EPrints ID: 425937
URI: http://eprints.soton.ac.uk/id/eprint/425937
ISSN: 1064-3745
PURE UUID: 3785f942-1763-4566-9e40-be374707df4d
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Date deposited: 07 Nov 2018 17:30
Last modified: 18 Mar 2024 03:18
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Contributors
Author:
Karly Rai Rogers-Broadway
Author:
Laura I. Karydis
Author:
Rachel C. Dobson
Editor:
S. Malek
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