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A novel antibody combination to identify KIR2DS2(high) NK cells in KIR2DL3/L2/S2 heterozygous donors

A novel antibody combination to identify KIR2DS2(high) NK cells in KIR2DL3/L2/S2 heterozygous donors
A novel antibody combination to identify KIR2DS2(high) NK cells in KIR2DL3/L2/S2 heterozygous donors
The killer cell immunoglobulin‐like receptor (KIR) KIR2DS2 induces NK cell activation upon ligation and in genetic studies is associated with protection against certain cancers and viral infections. One of the difficulties in understanding KIR2DS2 has been that ligands have been hard to define. In part, this is because the high sequence homology between KIR2DS2 and KIR2DL3/KIR2DL2 has made it difficult to make antibodies that specifically detect NK cells expressing KIR2DS2. Using transfected NKL cells and primary human samples, we report the identification of a novel antibody combination which allows identification of NK cells with relatively high expression of KIR2DS2. This separation is sufficient to examine primary human NK cell activation in response to KIR2DS2 specific ligands.
2059-2310
32-35
Blunt, Matthew
b1109de3-6045-4bc3-bd77-6cf26504697d
Rettman, Pauline
d761e696-6680-4168-931d-4586a6062a58
Bastidas Legarda, Leidy, Yamile
66bd5603-1c85-4321-a129-20ced5b35e4c
Fulton, Rebecca
848aa9dc-797f-40f8-b63d-bca0d90f7b55
Capizzuto, Valentino
60004ac3-5726-44b2-8fe1-1e2366211ba9
Naiyer, Mohammed M
b4ac5546-4f7d-462c-9632-a60a552ea3b3
Traherne, James A.
d5b0f130-0294-4337-9b97-7dbdacc1542a
Khakoo, Salim
6c16d2f5-ae80-4d9b-9100-6bfb34ad0273
Blunt, Matthew
b1109de3-6045-4bc3-bd77-6cf26504697d
Rettman, Pauline
d761e696-6680-4168-931d-4586a6062a58
Bastidas Legarda, Leidy, Yamile
66bd5603-1c85-4321-a129-20ced5b35e4c
Fulton, Rebecca
848aa9dc-797f-40f8-b63d-bca0d90f7b55
Capizzuto, Valentino
60004ac3-5726-44b2-8fe1-1e2366211ba9
Naiyer, Mohammed M
b4ac5546-4f7d-462c-9632-a60a552ea3b3
Traherne, James A.
d5b0f130-0294-4337-9b97-7dbdacc1542a
Khakoo, Salim
6c16d2f5-ae80-4d9b-9100-6bfb34ad0273

Blunt, Matthew, Rettman, Pauline, Bastidas Legarda, Leidy, Yamile, Fulton, Rebecca, Capizzuto, Valentino, Naiyer, Mohammed M, Traherne, James A. and Khakoo, Salim (2019) A novel antibody combination to identify KIR2DS2(high) NK cells in KIR2DL3/L2/S2 heterozygous donors. HLA, 32-35. (doi:10.1111/tan.13413).

Record type: Article

Abstract

The killer cell immunoglobulin‐like receptor (KIR) KIR2DS2 induces NK cell activation upon ligation and in genetic studies is associated with protection against certain cancers and viral infections. One of the difficulties in understanding KIR2DS2 has been that ligands have been hard to define. In part, this is because the high sequence homology between KIR2DS2 and KIR2DL3/KIR2DL2 has made it difficult to make antibodies that specifically detect NK cells expressing KIR2DS2. Using transfected NKL cells and primary human samples, we report the identification of a novel antibody combination which allows identification of NK cells with relatively high expression of KIR2DS2. This separation is sufficient to examine primary human NK cell activation in response to KIR2DS2 specific ligands.

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More information

Accepted/In Press date: 30 October 2018
e-pub ahead of print date: 1 November 2018
Published date: January 2019

Identifiers

Local EPrints ID: 425953
URI: https://eprints.soton.ac.uk/id/eprint/425953
ISSN: 2059-2310
PURE UUID: 15262c05-0432-4f8f-9a20-ae052ca75822
ORCID for Mohammed M Naiyer: ORCID iD orcid.org/0000-0002-4441-311X

Catalogue record

Date deposited: 07 Nov 2018 17:30
Last modified: 03 Dec 2019 05:09

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Contributors

Author: Matthew Blunt
Author: Pauline Rettman
Author: Leidy, Yamile Bastidas Legarda
Author: Rebecca Fulton
Author: Valentino Capizzuto
Author: Mohammed M Naiyer ORCID iD
Author: James A. Traherne
Author: Salim Khakoo

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