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In-vitro efficacy of antibiotics released from calcium sulfate bone void filler beads

In-vitro efficacy of antibiotics released from calcium sulfate bone void filler beads
In-vitro efficacy of antibiotics released from calcium sulfate bone void filler beads
Fifteen different antibiotics were individually mixed with a commercially available calcium sulfate bone void filler beads. The antibiotics were: amikacin, ceftriaxone, cefuroxime, ciprofloxacin, clindamycin, colistamethate sodium, daptomycin, gentamicin, imipenem/cilastatin, meropenem, nafcillin, rifampicin, teicoplanin, tobramycin and vancomycin. The efficacy of specific released antibiotics were validated by zone of inhibition (ZOI) testing using a modified Kirby—Bauer disk diffusion method against common periprosthetic joint infection pathogens. With a subset of experiments (daptomycin, rifampin, vancomycin alone and rifampin and vancomycin in combination) we investigated how release varied over 15 days using a repeated ZOI assay. We also tested the ability of these beads to kill biofilms formed by Staphylococcus epidermidis 35984, a prolific biofilm former. The results suggest that certain antibiotics can be combined and released from calcium sulfate with retained antibacterial efficacy. The daptomycin and rifampin plus vancomycin beads showed antimicrobial efficacy for the full 15 days of testing and vancomycin in combination with rifampin prevented resistant mutants. In the biofilm killing assay all of the antibiotic combinations showed a significant reduction in biofilm bacteria after 24 hr. The exposure time was an important factor in the amount of killing and varied among the antibiotics.
Calcium sulfate, Antibiotics, Release, Zone of Inhibition, Biofilm
Laycock, Phillip A.
bf34e2e9-d2fd-4128-b671-7f3e25d80d8a
Cooper, John J.
a97bc933-a0a4-4c97-bd6c-e32d6a4988ff
Howlin, Robert P.
f3c84990-6196-47d4-ad8a-80954ea46c7f
Delury, Craig
3b6f0751-f41a-4480-b470-3ce001bba84a
Stoodley, Paul
08614665-92a9-4466-806e-20c6daeb483f
Laycock, Phillip A.
bf34e2e9-d2fd-4128-b671-7f3e25d80d8a
Cooper, John J.
a97bc933-a0a4-4c97-bd6c-e32d6a4988ff
Howlin, Robert P.
f3c84990-6196-47d4-ad8a-80954ea46c7f
Delury, Craig
3b6f0751-f41a-4480-b470-3ce001bba84a
Stoodley, Paul
08614665-92a9-4466-806e-20c6daeb483f

Laycock, Phillip A., Cooper, John J., Howlin, Robert P., Delury, Craig and Stoodley, Paul (2018) In-vitro efficacy of antibiotics released from calcium sulfate bone void filler beads. Materials, 11 (11), [2265]. (doi:10.3390/ma11112265).

Record type: Article

Abstract

Fifteen different antibiotics were individually mixed with a commercially available calcium sulfate bone void filler beads. The antibiotics were: amikacin, ceftriaxone, cefuroxime, ciprofloxacin, clindamycin, colistamethate sodium, daptomycin, gentamicin, imipenem/cilastatin, meropenem, nafcillin, rifampicin, teicoplanin, tobramycin and vancomycin. The efficacy of specific released antibiotics were validated by zone of inhibition (ZOI) testing using a modified Kirby—Bauer disk diffusion method against common periprosthetic joint infection pathogens. With a subset of experiments (daptomycin, rifampin, vancomycin alone and rifampin and vancomycin in combination) we investigated how release varied over 15 days using a repeated ZOI assay. We also tested the ability of these beads to kill biofilms formed by Staphylococcus epidermidis 35984, a prolific biofilm former. The results suggest that certain antibiotics can be combined and released from calcium sulfate with retained antibacterial efficacy. The daptomycin and rifampin plus vancomycin beads showed antimicrobial efficacy for the full 15 days of testing and vancomycin in combination with rifampin prevented resistant mutants. In the biofilm killing assay all of the antibiotic combinations showed a significant reduction in biofilm bacteria after 24 hr. The exposure time was an important factor in the amount of killing and varied among the antibiotics.

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Accepted/In Press date: 10 November 2018
e-pub ahead of print date: 13 November 2018
Published date: 13 November 2018
Keywords: Calcium sulfate, Antibiotics, Release, Zone of Inhibition, Biofilm

Identifiers

Local EPrints ID: 426140
URI: http://eprints.soton.ac.uk/id/eprint/426140
PURE UUID: dfcc24a2-29ef-44d8-9d72-c282ed3fb52c
ORCID for Paul Stoodley: ORCID iD orcid.org/0000-0001-6069-273X

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Date deposited: 15 Nov 2018 17:30
Last modified: 17 Dec 2019 01:42

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Contributors

Author: Phillip A. Laycock
Author: John J. Cooper
Author: Robert P. Howlin
Author: Craig Delury
Author: Paul Stoodley ORCID iD

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