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Biosurfactant coated silver and iron oxide nanoparticles with enhanced anti-biofilm and anti-adhesive properties

Biosurfactant coated silver and iron oxide nanoparticles with enhanced anti-biofilm and anti-adhesive properties
Biosurfactant coated silver and iron oxide nanoparticles with enhanced anti-biofilm and anti-adhesive properties

Pseudomonas aeruginosa and Staphylococcus aureus are among the hazardous biofilm forming bacteria ubiquitous in industrial/clinical wastes. Serious efforts are required to develop effective strategies to control surface-growing antibiotic resistant pathogenic bacterial communities which they are emerging as a global health issue. Blocking hazardous biofilms would be a useful aspect of biosurfactant coated nanoparticles (NPs). In this regard, we report a facile method for the synthesis of rhamnolipid (RL) coated silver (Ag) and iron oxide (Fe3O4) NPs and propose the mechanism of their synergistic antibacterial and anti-adhesive properties against biofilms formed by P. aeruginosa and S. aureus. These NPs demonstrated excellent anti-biofilm activity not only during the biofilms formation but also on the pre-formed biofilms. Mechanistically, RL coated silver (35 nm) and Fe3O4 NPs (48 nm) generate reactive oxygen species, which contribute to the antimicrobial activity. The presence of RLs shell on the nanoparticles significantly reduces the cell adhesion by modifying the surface hydrophobicity and hence enhancing the anti-biofilm property of NPs against both mentioned strains. These findings suggest that RL coated Ag and Fe3O4 NPs may be used as potent alternate to reduce the infection severity by inhibiting the biofilm formation and, therefore, they possess potential biomedical applications for antibacterial coatings and wound dressings.

Antiadhesive property, Antimicrobial activity, Biofilm eradication, Iron oxide nanoparticles, Rhamnolipids, Silver nanoparticles
0304-3894
441-448
Khalid, Hafiza Faiza
eecf4f89-bebe-41e3-a69a-d8b4e95daf98
Tehseen, Bushra
cb5e224f-2e0f-44c6-bcbf-9d3f5f20f407
Sarwar, Yasra
596ea07a-0950-44a0-b315-113e8705da50
Hussain, Syed Zajif
568fb531-4eb7-4da5-a33b-0ed9cae07d91
Khan, Waheed S.
ecc7d05e-4a3f-4dfd-a308-972f1c6c2018
Raza, Zulfiqar Ali
53cb8043-130a-46ad-bdd3-79ab716187a0
Bajwa, Sadia Zafar
41f5d82e-ce72-457b-8705-2c252528d72b
Kanaras, Antonios G.
667ecfdc-7647-4bd8-be03-a47bf32504c7
Hussain, Irshad
6fcf2f10-9969-4d33-9169-ca89845ef720
Rehman, Asma
997780e5-3612-4fb0-92c7-22810b1711e7
Khalid, Hafiza Faiza
eecf4f89-bebe-41e3-a69a-d8b4e95daf98
Tehseen, Bushra
cb5e224f-2e0f-44c6-bcbf-9d3f5f20f407
Sarwar, Yasra
596ea07a-0950-44a0-b315-113e8705da50
Hussain, Syed Zajif
568fb531-4eb7-4da5-a33b-0ed9cae07d91
Khan, Waheed S.
ecc7d05e-4a3f-4dfd-a308-972f1c6c2018
Raza, Zulfiqar Ali
53cb8043-130a-46ad-bdd3-79ab716187a0
Bajwa, Sadia Zafar
41f5d82e-ce72-457b-8705-2c252528d72b
Kanaras, Antonios G.
667ecfdc-7647-4bd8-be03-a47bf32504c7
Hussain, Irshad
6fcf2f10-9969-4d33-9169-ca89845ef720
Rehman, Asma
997780e5-3612-4fb0-92c7-22810b1711e7

Khalid, Hafiza Faiza, Tehseen, Bushra, Sarwar, Yasra, Hussain, Syed Zajif, Khan, Waheed S., Raza, Zulfiqar Ali, Bajwa, Sadia Zafar, Kanaras, Antonios G., Hussain, Irshad and Rehman, Asma (2019) Biosurfactant coated silver and iron oxide nanoparticles with enhanced anti-biofilm and anti-adhesive properties. Journal of Hazardous Materials, 364, 441-448. (doi:10.1016/j.jhazmat.2018.10.049).

Record type: Article

Abstract

Pseudomonas aeruginosa and Staphylococcus aureus are among the hazardous biofilm forming bacteria ubiquitous in industrial/clinical wastes. Serious efforts are required to develop effective strategies to control surface-growing antibiotic resistant pathogenic bacterial communities which they are emerging as a global health issue. Blocking hazardous biofilms would be a useful aspect of biosurfactant coated nanoparticles (NPs). In this regard, we report a facile method for the synthesis of rhamnolipid (RL) coated silver (Ag) and iron oxide (Fe3O4) NPs and propose the mechanism of their synergistic antibacterial and anti-adhesive properties against biofilms formed by P. aeruginosa and S. aureus. These NPs demonstrated excellent anti-biofilm activity not only during the biofilms formation but also on the pre-formed biofilms. Mechanistically, RL coated silver (35 nm) and Fe3O4 NPs (48 nm) generate reactive oxygen species, which contribute to the antimicrobial activity. The presence of RLs shell on the nanoparticles significantly reduces the cell adhesion by modifying the surface hydrophobicity and hence enhancing the anti-biofilm property of NPs against both mentioned strains. These findings suggest that RL coated Ag and Fe3O4 NPs may be used as potent alternate to reduce the infection severity by inhibiting the biofilm formation and, therefore, they possess potential biomedical applications for antibacterial coatings and wound dressings.

Text
Biosurfactant coated silver and iron oxide nanoparticles with enhanced anti-biofilm and anti-adhesive properties - Accepted Manuscript
Restricted to Repository staff only until 19 October 2019.
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More information

Accepted/In Press date: 16 October 2018
e-pub ahead of print date: 19 October 2018
Published date: 15 February 2019
Keywords: Antiadhesive property, Antimicrobial activity, Biofilm eradication, Iron oxide nanoparticles, Rhamnolipids, Silver nanoparticles

Identifiers

Local EPrints ID: 426444
URI: https://eprints.soton.ac.uk/id/eprint/426444
ISSN: 0304-3894
PURE UUID: 717b051d-3988-4296-86ef-45fe900e1e84
ORCID for Antonios G. Kanaras: ORCID iD orcid.org/0000-0002-9847-6706

Catalogue record

Date deposited: 27 Nov 2018 17:30
Last modified: 14 Mar 2019 01:39

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Contributors

Author: Hafiza Faiza Khalid
Author: Bushra Tehseen
Author: Yasra Sarwar
Author: Syed Zajif Hussain
Author: Waheed S. Khan
Author: Zulfiqar Ali Raza
Author: Sadia Zafar Bajwa
Author: Irshad Hussain
Author: Asma Rehman

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