READ ME File For 'Raw Data for DNA – Coated Gold Nanoparticles for the Detection of mRNA in Live Hydra Vulgaris Animals'

Dataset https://doi.org/10.5258/SOTON/D0727 

ReadMe Author: Maria-Eleni Kyriazi, University of Southampton

This dataset supports the publication:


"DNA – Coated Gold Nanoparticles for the Detection of mRNA in Live Hydra Vulgaris Animals"


Contents


All experimental data recorded and used to generate the above title manuscript and submitted to ACS Applied Materials and Interfaces



Folder 1 -  Confocal Microscopy Images: Confocal microscopy images presented in the main manuscript of live Hydra Vulgaris animals treated with gold nanoparticle nanoprobes 

Folder 2 Ð Melting Curves: Contains raw data used to plot the melting curves presented in the Supporting Information showing qualitative hybridization of flare/sense strand

Folder 3 Ð Quantitative Oligonucleotide Loading: Contains excel document with data used to assess the quantitative loading of the ÔsenseÕ strand onto the gold nanoparticle surface as well as the number of ÔflareÕ strands per gold nanoparticle

Folder 4 Ð UV-Vis data:  Contains raw data used to plot the UV-Vis spectra for each type of nanoprobe used in this study and presented in the Supporting Information 

Folder 5 Ð Zeta Potential Measurements: Contains an excel folder with the zeta potential spectra for each nanoprobe


Geographic location of data collection: University of Southampton, U.K.

Related projects:

BBSRC Grant BB/P017711/1
Horizon 2020 research and innovation programme (Marie Sklodowska-Curie grant agreement No. 660228).


Dataset available under a CC BY 4.0 licence


Publisher: University of Southampton, U.K.


Date: December 2018