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A novel microfluidic point-of-care biosensor system on printed circuit board for cytokine detection

A novel microfluidic point-of-care biosensor system on printed circuit board for cytokine detection
A novel microfluidic point-of-care biosensor system on printed circuit board for cytokine detection

Point of Care (PoC) diagnostics have been the subject of considerable research over the last few decades driven by the pressure to detect diseases quickly and effectively and reduce healthcare costs. Herein, we demonstrate a novel, fully integrated, microfluidic amperometric enzyme-linked immunosorbent assay (ELISA) prototype using a commercial interferon gamma release assay (IGRA) as a model antibody binding system. Microfluidic assay chemistry was engineered to take place on Au-plated electrodes within an assay cell on a printed circuit board (PCB)-based biosensor system. The assay cell is linked to an electrochemical reporter cell comprising microfluidic architecture, Au working and counter electrodes and a Ag/AgCl reference electrode, all manufactured exclusively via standard commercial PCB fabrication processes. Assay chemistry has been optimised for microfluidic diffusion kinetics to function under continual flow. We characterised the electrode integrity of the developed platforms with reference to biological sampling and buffer composition and subsequently we demonstrated concentration-dependent measurements of H₂O₂ depletion as resolved by existing FDA-validated ELISA kits. Finally, we validated the assay technology in both buffer and serum and demonstrate limits of detection comparable to high-end commercial systems with the addition of full microfluidic assay architecture capable of returning diagnostic analyses in approximately eight minutes.

cytokine detection, eELISA, lab-on-PCB, microfluidics, PCB biosensors, point-of-care diagnostics
1424-8220
1-14
Evans, Daniel
331a6db3-9b2e-4659-80ce-ae8ae7ddc1b5
Papadimitriou, Konstantinos I.
c0535540-f862-41b1-9cf3-92b1f46a4145
Vasilakis, Nikolaos
7b5d4280-8c8d-4e55-8d58-6a38ab5c0bb2
Pantelidis, Panagiotis
35ea018c-f5e2-498c-8741-84a66b5e2828
Kelleher, Peter
c6b66f9b-bf9f-42a6-aad9-7dfc1cda87e7
Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174
Prodromakis, Themistoklis
d58c9c10-9d25-4d22-b155-06c8437acfbf
Evans, Daniel
331a6db3-9b2e-4659-80ce-ae8ae7ddc1b5
Papadimitriou, Konstantinos I.
c0535540-f862-41b1-9cf3-92b1f46a4145
Vasilakis, Nikolaos
7b5d4280-8c8d-4e55-8d58-6a38ab5c0bb2
Pantelidis, Panagiotis
35ea018c-f5e2-498c-8741-84a66b5e2828
Kelleher, Peter
c6b66f9b-bf9f-42a6-aad9-7dfc1cda87e7
Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174
Prodromakis, Themistoklis
d58c9c10-9d25-4d22-b155-06c8437acfbf

Evans, Daniel, Papadimitriou, Konstantinos I., Vasilakis, Nikolaos, Pantelidis, Panagiotis, Kelleher, Peter, Morgan, Hywel and Prodromakis, Themistoklis (2018) A novel microfluidic point-of-care biosensor system on printed circuit board for cytokine detection. Sensors, 18 (11), 1-14. (doi:10.3390/s18114011).

Record type: Article

Abstract

Point of Care (PoC) diagnostics have been the subject of considerable research over the last few decades driven by the pressure to detect diseases quickly and effectively and reduce healthcare costs. Herein, we demonstrate a novel, fully integrated, microfluidic amperometric enzyme-linked immunosorbent assay (ELISA) prototype using a commercial interferon gamma release assay (IGRA) as a model antibody binding system. Microfluidic assay chemistry was engineered to take place on Au-plated electrodes within an assay cell on a printed circuit board (PCB)-based biosensor system. The assay cell is linked to an electrochemical reporter cell comprising microfluidic architecture, Au working and counter electrodes and a Ag/AgCl reference electrode, all manufactured exclusively via standard commercial PCB fabrication processes. Assay chemistry has been optimised for microfluidic diffusion kinetics to function under continual flow. We characterised the electrode integrity of the developed platforms with reference to biological sampling and buffer composition and subsequently we demonstrated concentration-dependent measurements of H₂O₂ depletion as resolved by existing FDA-validated ELISA kits. Finally, we validated the assay technology in both buffer and serum and demonstrate limits of detection comparable to high-end commercial systems with the addition of full microfluidic assay architecture capable of returning diagnostic analyses in approximately eight minutes.

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More information

Accepted/In Press date: 12 November 2018
e-pub ahead of print date: 12 November 2018
Published date: 17 November 2018
Keywords: cytokine detection, eELISA, lab-on-PCB, microfluidics, PCB biosensors, point-of-care diagnostics

Identifiers

Local EPrints ID: 426646
URI: https://eprints.soton.ac.uk/id/eprint/426646
ISSN: 1424-8220
PURE UUID: a12dd8b7-b18a-40c3-acc4-1353fe636aed
ORCID for Hywel Morgan: ORCID iD orcid.org/0000-0003-4850-5676
ORCID for Themistoklis Prodromakis: ORCID iD orcid.org/0000-0002-6267-6909

Catalogue record

Date deposited: 07 Dec 2018 17:30
Last modified: 15 Aug 2019 00:45

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Contributors

Author: Daniel Evans
Author: Konstantinos I. Papadimitriou
Author: Nikolaos Vasilakis
Author: Panagiotis Pantelidis
Author: Peter Kelleher
Author: Hywel Morgan ORCID iD

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