READ ME File For 'Dataset for Sample pre-concentration on a digital microfluidic platform for rapid AMR detection in urine' Dataset DOI: 10.5258/SOTON/D0731 This dataset supports the publication: Kalsi, S. et al (2018). Sample pre-concentration on a digital microfluidic platform for rapid AMR detection in urine. Lab on a Chip., DOI: 10.1039/C8LC01249K Contents +++++++++ For example This dataset contains which are used for generating figures in the paper above. The figures are as follows: Fig. 4a Effect of the addition of unfiltered urine to the RPA reaction mixture (1:50 dilution) plotted as a change in TTP compared with the mean TTP of the positive control (purified K. Pneumonia plasmid DNA, 90,000 copies). The DNA was added to RPA reaction mix Fig. 4b Capture efficiency of plasmid DNA onto the beads at different concentrations of GuHCl in PBS and urine, quantified as a change in TTP compared with the positive control. Purified plasmid DNA (90,000 copies) was added to either phosphate buffer saline (PBS) or urine containing different concentration of GuHCl and captured on 2.5 µL of magnetic beads. Plasmid was eluted into 5µL of elution buffer. Experiments were performed using the protocol shown in Figure 1A. Data from duplicates. Fig. 5a RPA amplification curves for DNA extracted from Klebsiella pneumoniae NCTC 13443 using the benchtop protocol shown in Figure 1. Fig. 5b Plot of time to positivity (TTP) vs log10 bacteria concentration. Data from duplicates. Fig. 6b RPA amplification curve for the image shown in Fig 6(A) Fig. 6c RPA amplification curves for purified DNA loaded directly on reservoir electrodes. Fig. 6d TTP with respect to DNA concentration. Data is average of duplicates shown in Fig 6C Fig. 7c RPA amplification curve for two different bacteria concentrations in urine from two healthy volunteers. Fig. 7d RPA amplification curve for two different bacteria concentrations in urine from two healthy volunteers. Fig. S3 Plot of time to positivity (TTP) vs log10 purified DNA (genomic and plasmids) concentration, extracted using DNeasy Blood and Tissue Kit (Qiagen, UK). Data is average of duplicates. Fig. S4 Plot of amount of purified DNA (in ng) added to 3M GuHCl (1 mL) and processed on DMF platform using described protocol. The beads are re-suspended into 5 µL of elution buffer and quantified using a nanodrop. Geographic location of data collection: University of Southampton, U.K. Related projects: Rapid Detection of Infectious Agents at Point of Triage Morgan, H. NIHR Clinical Research Network Co-ordinating Centre Dataset available under a CC BY 4.0 licence Publisher: University of Southampton, U.K. Date: December 2018