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[(cu)19.7(lt)20.6(ur)15.1(ed)-279.8(wi)19.8(th)20.7(ou)20.8(t)-295(f)0(e)19.7(e)0(d)19.7(e)0(r)-279.8(c)0(e)18.5(l)0(l)20.6(s)-294.6(on)-278.7(Ma)18.8(tr)20.7(ig)20.7(el)-285.7(\(B)19.8(ec)18.5(to)20.7(n)-289.1(D)0(i)19.8(c)0(k)13.9(i)0(n)20.7(s)0(o)21(n)0(,)]TJ
T*
[(He)18.8(id)20.7(el)13.8(be)19.7(rg)20.9(,)-354.8(G)0(e)18.8(r)0(m)19.6(a)0(n)19.7(y)0(\))-342(u)0(s)21(i)0(n)15(g)-352.4(St)20.9(em)18.5(MA)19.1(CS)-342.9(iP)20.9(S-)21(Br)14.1(ew)-344.1(XF)20.1(,)-354.8(h)0(u)20.8(-)]TJ
0 -1.1174 TD
.0117 Tc
[(man)-260.1(\()-81.5(Mi)4.7(lteny)4.8(i)-260.2(B)-1.9(io)4.8(tec,)-262.4(Ber)4.9(g)-.9(i)4.7(s)-.7(ch)-265.9(Gladbach)4.8(,)-262.4(G)4(erman)4.8(y)-.9(\))4.9(,)-262.4(wi)4.7(th)]TJ
0 -1.1116 TD
.0122 Tc
[(1%)-318.3(peni)5.2(ci)5.2(ll)5.2(in/)5.2(s)-.2(t)5.2(r)-.4(ep)5.3(to)5.3(my)5.3(ci)5.2(n)-323(i)5.2(n)-323(h)5.3(umid)5.3(ied)-323(atmos)5.5(p)-.4(h)5.3(e)-1.6(re)-318.4(wi)5.2(th)]TJ
T*
.0103 Tc
[(5%)-268.3(CO)]TJ
7.3823 0 0 6.5614 91.8425 142.6393 Tm
0 Tc
(2)Tj
9.843 0 0 9.843 98.3055 144.2834 Tm
.0075 Tc
[(at)-276(37)]TJ
/F9 1 Tf
2.0389 0 TD
0 Tc
()Tj
/F4 1 Tf
.4032 0 TD
.0123 Tc
[(C,)-267.6(with)-265.3(dail)5.3(y)-271(c)4.3(hang)5.4(e)-272.2(o)5.4(f)-271(m)4.2(ed)5.4(iu)5.4(m.)]TJ
-5.1146 -1.1174 TD
0 Tc
[(When)-307.3(conuency)-307(was)-310.4(reached,)-309.5(the)-309.9(medium)-304.1(was)-310.4(changed)]TJ
-1.0137 -1.1116 TD
(to)Tj
/F3 1 Tf
1.0022 0 TD
(a)Tj
/F4 1 Tf
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[(-M)20(EM)-223.2(\(s)21.1(ee)-223.4(fM)20(SC)-221.9(m)15(e)0(d)13.9(i)0(u)20.7(m)0(\))-216.5(a)0(n)13.9(d)-225.7(th)20.7(e)-232.6(p)0(l)20.7(u)0(r)20.9(i)0(p)15(o)0(t)20.7(e)0(n)19.7(t)-231.6(ce)18.5(ll)20.6(s)-231.3(w)0(e)18.8(r)0(e)]TJ
-1.5436 -1.1116 TD
[(tr)20.7(ea)18.5(te)13.8(d)-283.3(d)0(a)19.7(i)0(l)20.6(y)-283.3(wi)19.8(th)-273(10)]TJ
/F3 1 Tf
8.4034 0 TD
(m)Tj
/F4 1 Tf
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[(M)-284.2(o)0(f)-272.9(t)0(h)20.7(e)-284.5(tr)20.7(an)13.9(sf)21.1(or)20.9(mi)19.5(ng)-272.9(gr)15.1(ow)20(th)-273(fa)19.7(ct)19.5(or)15.1(-)]TJ
/F3 1 Tf
14.7967 0 TD
(b)Tj
/F4 1 Tf
-23.7415 -1.1116 TD
[(\(T)19.7(GF)20.1(-)]TJ
/F3 1 Tf
2.5055 0 TD
(b)Tj
/F4 1 Tf
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[(\))-329.4(r)0(e)14(c)0(e)18.5(p)0(t)20.7(o)0(r)-324.7(i)0(n)20.7(h)0(i)20.7(b)0(i)20.7(t)0(o)20.7(r)-335.1(SB)20(-4)15.1(31)20.8(54)20.8(2)-335.2(\()-70.2(Mi)19.8(lt)20.6(en)13.9(yi)-319.1(Bi)13.9(ot)20.7(ec)18.5(\))-329.4(f)0(o)15.1(r)]TJ
-3.0469 -1.1174 TD
[(14)-261.4(da)19.7(ys)21(.)-274.1(A)0(f)20(t)0(e)19.6(r)0(w)20(a)0(r)14(d)0(,)-258(t)0(h)20.7(e)-273(em)18.5(er)19.7(ge)19.7(d)-271.8(i)0(M)19.8(S)0(C)14.2(s)-271.6(we)18.8(re)-262.5(tr)20.7(yp)20.8(si)20.9(ni)15(ze)18.5(d)-266(u)0(s)15.3(-)]TJ
0 -1.1116 TD
[(in)20.7(g)-168.1(T)0(r)19.7(y)0(p)20.8(L)0(E)-154.3(E)0(x)13.9(p)0(r)20.9(e)0(s)19.9(s)-167.9(\(T)19.7(he)19.7(rm)19.6(o)-162.4(F)0(i)15.1(s)0(h)21(e)0(r)-158.8(S)0(c)19.8(i)0(e)19.6(n)0(t)20.7(i)0()20.9(c)0(,)-155.4(W)0(a)17.4(l)0(t)14.8(h)0(a)19.7(m)0(,)-155.5(M)0(A)19.1(\))]TJ
T*
[(fo)20.9(r)-237.2(1)0(0)-146.2(m)0(i)19.5(n)-237.2(at)-228.1(37)]TJ
/F9 1 Tf
6.2435 0 TD
()Tj
/F4 1 Tf
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[(C,)-230.2(ce)18.5(nt)20.7(ri)20.7(fu)20.9(ge)19.7(d)-237.2(a)0(t)-228.1(3)0(0)20.8(0)]TJ
/F5 1 Tf
8.5244 0 TD
(g)Tj
/F4 1 Tf
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[(fo)20.9(r)-237.2(5)-156.6(mi)19.5(n,)-223.4(an)13.9(d)-231.5(s)0(e)14.1(e)0(d)19.7(e)0(d)]TJ
-15.891 -1.1174 TD
[(on)20.8(to)-342.1(un)20.8(co)19.7(at)19.5(ed)-337.4(cu)19.7(lt)14.8(ur)20.9(e)-347.8(d)0(i)20.7(s)0(h)15.3(e)0(s)-337.2(i)0(n)-336.4(a)0(n)-337.4(M)0(S)14.3(C)-347.7(ex)19.7(pa)19.7(ns)21(io)20.7(n)-352.4(m)0(e)18.4(d)0(i)20.7(u)0(m)]TJ
25.504 67.9242 TD
[(\(s)15.3(ee)-275.2(fM)20(SC)-279.5(me)18.4(di)20.7(um)19.6(\),)-275.2(wh)20(ic)19.5(h)-289.1(l)0(e)19.6(d)-283.3(to)-278.8(th)20.7(e)-284.5(d)0(e)13.9(p)0(l)20.7(e)0(t)19.5(i)0(o)20.7(n)-289.1(of)-272.9(ce)18.5(ll)20.6(s)-288.9(w)0(i)19.8(t)0(h)]TJ
0 -1.1174 TD
[(ma)18.4(in)15(ta)19.5(in)20.7(ed)-158.9(iP)15.1(SC)-158.6(ch)19.7(ar)19.7(ac)18.5(te)19.5(ri)15(st)20.9(ic)19.6(s.)-159.8(Fu)21(rt)15(he)19.7(r)-168.1(p)0(a)19.7(s)0(s)15.5(a)0(g)19.7(i)0(n)20.7(g)-168.1(wa)18.8(s)-173.7(c)0(a)18.5(r)0(r)20.9(i)0(e)19.5(d)]TJ
0 -1.1116 TD
[(ou)15.1(t)-369.8(a)0(s)-360.3(d)0(e)19.7(s)0(c)19.9(r)0(i)20.7(b)0(e)19.7(d)-369.7(ab)13.9(ov)20.8(e)-370.9(w)0(h)20(e)0(n)-360.5(c)0(e)18.5(l)0(l)20.6(s)-369.5(re)19.7(ac)18.5(he)13.9(d)-369.7(a)0(b)19.7(o)0(u)20.8(t)-369.9(95)20.8(%)-370.8(c)0(o)19.7(n)0(-)]TJ
T*
[(u)15.2(en)19.7(cy)19.7(.)-262.6(T)0(h)19.6(e)-261.4(ce)18.5(ll)20.6(s)-260.1(w)0(e)18.8(r)0(e)-251(s)0(e)19.9(e)0(d)19.7(e)0(d)-251(a)0(t)-251.2(1)]TJ
/F9 1 Tf
14.0191 0 TD
()Tj
/F4 1 Tf
.7142 0 TD
(10)Tj
7.3823 0 0 6.5614 467.7164 696.6423 Tm
(6)Tj
9.843 0 0 9.843 473.9526 692.277 Tm
[(ce)18.5(ll)20.6(s)-260.1(p)0(e)13.9(r)-260.2(17)20.8(5)-156.6(c)0(m)]TJ
7.3823 0 0 6.5614 537.2786 696.6423 Tm
(2)Tj
9.843 0 0 9.843 540.8503 692.277 Tm
(.)Tj
/F10 1 Tf
9.9626 0 0 9.9626 313.0582 669.0329 Tm
[(Tra)-9(nscriptom)-9.5(e)-330(anal)-9.6(ysis)]TJ
/F4 1 Tf
9.843 0 0 9.843 323.0361 651.1746 Tm
[(Microarray)-470.5(experiments)-466.1(were)-468.3(performed)-462.6(on)-463(the)-465.4(Prime-)]TJ
-1.0137 -1.1116 TD
[(View)-289.7(Human)-285.2(Gene)-284.1(Expression)-283.8(Array)-289.7(platform)-282.1(\(Affymetrix;)]TJ
0 -1.1174 TD
[(Thermo)-419.1(Fisher)-410.7(Scientic\),)-421.2(the)-413.5(data)-415.8(are)-414.5(provided)-413.2(online)-411.2(at)]TJ
0 -1.1116 TD
[(the)-396.3(National)-392.6(Center)-390.2(of)-393.8(Biotechnology)-392.5(Information)-394.8(\(NCBI\))]TJ
T*
[(Gene)-479.9(Expression)-479.6(Omnibus)-476.3(\(https://www.ncbi.nlm.nih.gov/)]TJ
0 -1.1174 TD
-.0215 Tc
[(g)-5.3(e)-.7(o/qu)-5.3(ery/ac)-6.5(c.c)-6.5(g).4(i?acc=)-6.7(GSE976)-5.3(92\).)-537.5(T)-.8(he)-536.4(u)-5.3(nnorma)-6.5(lized)-535.2(b)-5.3(ead-)]TJ
0 -1.1116 TD
.0121 Tc
[(s)28.5(u)34(m)32.8(m)32.8(a)32.9(r)34.1(y)-386.4(data)-416.4(wer)5.3(e)-422.1(f)5.3(u)-.5(r)5.3(t)-.6(her)-420.9(p)5.2(ro)5.2(cessed)-421(t)5.1(hro)5.2(ug)5.2(h)-421(t)5.1(he)-416.4(R/)5.1(Bio)5.2(-)]TJ
T*
.007 Tc
[(c)-6.8(o)-5.6(nd)-5.6(uc)-6.8(to)-5.6(r)-259(e)-6.8(nv)-5.6(ir)-5.6(o)-5.6(n).1(m)-6.8(e)-1(n)-5.6(t)-259.2(em)-6.8(pl)-5.7(oy)-5.6(in)-5.6(g)-259(t)-5.7(he)-260.2(p)-5.6(a)-1(c)-6.8(k).1(a)-6.8(g).1(e)-260.2(a)-6.8(ff)-5.6(y)-259(\()-5.6(ht)-5.7(tp)-5.6(:)-5.7(/)0(/)]TJ
T*
-.0217 Tc
[(b)-5.5(i).1(ocon)-5.5(ductor)-5.5(.)3.7(o)-5.5(r).3(g/pa)-6.7(ckages)-5.3(/relea)-6.7(se/bi)-5.6(o).2(c/html)-5.6(/affy)-5.5(.h)-5.5(tml\).)-526.2(U)-6.4(sing)]TJ
0 -1.1174 TD
0 Tc
[(thi)-9.3(s)-507.8(packa)-9(ge,)-513.7(the)-517.2(data)-513.7(were)-520.2(backg)-7.8(round-)-6.5(correc)-10(ted,)-514.9(trans-)]TJ
0 -1.1116 TD
[(for)14(med)-247.6(to)-250(a)-255.7(log)13.8(ari)12.7(thm)18.4(ic)-251.2(sca)11.8(le)-251.2(\(to)-245.3(the)-246.5(bas)13(e)-249.9(2\),)-247.5(and)-246.4(nor)14(ma)12.7(liz)12.6(ed)]TJ
T*
[(by)-388.1(ap)13.9(ply)13.9(ing)-377.8(the)-379(Rob)12.9(ust)-377.6(Mul)12.9(ti-)13.8(arr)12.9(ay)-383.5(Ave)11.9(rag)12.8(e)-388.2(nor)14(ma)12.7(liz)12.6(ati)12.6(on)]TJ
0 -1.1174 TD
[(met)11.4(hod)14(.)]TJ
/F10 1 Tf
9.9626 0 0 9.9626 313.0582 496.403 Tm
[(Im)-7(munophe)-9.1(notypin)-8.1(g)-330(b)0(y)-336.5(ow)-341.2(cytometry)]TJ
/F4 1 Tf
9.843 0 0 9.843 323.0361 478.5447 Tm
[(Th)19.6(e)-532.2(c)0(e)18.5(l)0(l)-526.6(s)0(u)21(r)0(f)20.9(a)0(c)24.3(e)-537.9(ma)18.4(rk)26.6(er)-527.5(ex)19.7(pr)20.9(es)19.9(si)20.9(o)16.2(n)-536.7(of)-526.3(th)26.5(e)-537.9(f)0(M)20(S)0(C)20(s)-530.8(an)19.7(d)]TJ
-1.0137 -1.1116 TD
[(iM)19.8(SC)20(s)-311.9(w)0(a)18.8(s)-311.9(an)19.7(al)19.5(yz)25.4(ed)-302.9(by)-301.7(us)21(in)20.7(g)-312.1(t)0(h)20.7(e)-313.3(hu)20.8(m)15(a)0(n)-302.9(M)0(S)20.1(C)-313.1(Ph)21(en)19.7(ot)26.5(yp)20.8(in)20.7(g)]TJ
T*
[(Ki)19.8(t)-237.4(H)0(u)20(m)15(an)-228(\(#)-221(13)20.8(0-)20.9(09)20.8(5-)20.9(19)26.6(8\))-226.8(fr)20.9(om)-222.3(Mi)19.8(lt)20.6(en)19.7(y)-231.5(B)0(i)19.7(o)0(t)20.7(e)0(c)24.3(.)-233.8(To)-228(co)19.7(n)16.2(d)0(u)20.8(c)0(t)]TJ
T*
[(th)20.7(is)-226.8(an)19.7(al)19.6(ys)21(is)20.9(,)-233.8(2)0(0)20.8(0)0(,)18.5(0)0(0)20.8(0)-237.2(d)16.2(e)0(t)19.5(a)0(c)18.5(h)0(e)19.7(d)-237.2(ce)18.5(l)16.1(l)0(s)-232.5(w)15.3(er)19.7(e)-238.4(d)0(i)20.7(s)0(t)20.9(r)0(i)20.7(b)0(u)20.8(t)0(e)19.6(d)-237.2(i)16.1(n)0(t)20.7(o)-237.2(tw)19.8(o)]TJ
0 -1.1174 TD
[(5)-156.6(m)0(L)-540.3()0(o)21(w)-543.4(cy)19.7(to)20.7(me)24.2(tr)20.7(y)-548.3(t)0(u)20.7(b)0(e)19.7(s)-548.1(i)16(n)-548.3(10)20.8(0)]TJ
/F3 1 Tf
14.9868 0 TD
(m)Tj
/F4 1 Tf
.5414 0 TD
-.0203 Tc
[(L)-564(p)-20.3(ho)-20.3(sp)-20.3(ha)-20.3(te)-20.3(-b)-20.3(u)6.3(f)-20.3(fe)-20.3(re)-20.3(d)]TJ
-15.5282 -1.1116 TD
0 Tc
[(sa)19.9(li)20.6(ne)-406.5(\(P)21(BS)20(\).)-401.9(Th)19.6(e)-417(p)0(h)20.8(e)15(no)20.8(ty)20.7(pi)20.7(ng)-405.4(co)19.7(c)15(k)0(t)20.7(a)0(i)19.5(l)-415.9(\(0)20.9(.)13.9(5)]TJ
/F3 1 Tf
18.2583 0 TD
(m)Tj
/F4 1 Tf
.5414 0 TD
[(L\))-406.5(co)19.7(nt)20.7(a)15(i)0(n)20.7(i)0(n)20.7(g)]TJ
-18.7997 -1.1116 TD
[(an)19.7(ti)20.6(bo)20.8(di)20.7(es)-302.7(ag)19.7(ai)19.5(n)16.2(s)0(t)-307.4(C)15.2(D1)20(4-)20.9(Pe)19.8(rC)19.8(P,)-298.1(CD)18.9(20)20.8(-P)21(er)19.7(CP)20(,)-308.7(C)0(D)18.9(3)0(4)20.8(-)0(P)21(e)0(r)19.7(C)0(P)20(,)]TJ
0 -1.1174 TD
[(CD)18.9(45)20.8(-P)21(er)19.7(CP)20(,)-493(C)0(D)24.7(7)0(3)20.8(-)0(A)20(P)0(C)20(,)-493(CD)18.9(90)20.8(-F)21(IT)25.4(C,)-483.6(an)19.7(d)-490.7(C)0(D)18.9(1)0(0)20.8(5)0(-)20.9(P)0(E)-481.3(o)0(r)]TJ
0 -1.1116 TD
[(th)20.7(e)-273(i)0(s)20.9(o)0(t)20.7(y)0(p)20.8(e)-267.2(co)19.7(nt)20.7(ro)20.9(l)-266.2(c)0(o)19.7(c)0(k)19.7(t)0(a)19.6(i)0(l)-255.9(w)0(a)18.8(s)-271.6(th)20.7(en)-256.8(ap)19.7(pl)20.7(ie)19.5(d.)-258(Af)20(te)25.3(r)-271.8(1)0(0)-146.2(m)0(i)19.5(n)-266(of)]TJ
T*
[(in)20.7(cu)19.7(ba)19.7(ti)20.6(on)-295.9(a)15(t)-306.5(4)]TJ
/F9 1 Tf
5.9613 0 TD
()Tj
/F4 1 Tf
.3859 0 TD
[(C,)-293.5(th)20.7(e)-307.5(c)0(e)18.5(l)0(l)20.6(s)-306.2(w)15.3(e)0(r)19.7(e)-307.5(wa)18.8(sh)21(ed)-291.3(an)19.7(d)-306.4(r)0(e)19.7(s)0(u)21(s)0(p)21(e)0(n)19.7(d)0(e)25.4(d)-306.4(in)]TJ
-6.3472 -1.1174 TD
[(10)20.8(0)]TJ
/F3 1 Tf
1.6358 0 TD
(m)Tj
/F4 1 Tf
.5414 0 TD
[(L)-584(p)0(a)19.7(r)0(a)19.7(f)0(o)20.9(r)0(m)25.4(a)0(l)19.5(d)0(e)19.7(h)0(y)20.8(d)0(e)-573.6(\()0(P)21(F)0(A)-573.1(4)16.2(%\))-573.4(fo)20.9(r)-582.8()0(o)21(w)-583.7(cy)19.7(to)20.7(m)14.9(e)0(t)19.6(r)0(i)20.7(c)]TJ
-2.1772 -1.1116 TD
[(an)19.7(al)19.5(ys)21(is)-520.5(\(F)21(AC)18.9(S)-530.8(C)0(a)18.6(n)0(t)20.7(o)-531(fr)20.9(om)-521.8(BD)-522.5(Bi)19.7(os)21(ci)19.5(en)19.7(ce)18.5(s,)-516.9(He)18.8(id)20.7(el)19.5(be)19.7(rg)20.9(,)]TJ
T*
[(Ge)18.8(rm)19.6(an)19.7(y\))20.9(.)-400.8(T)0(h)19.6(e)-405.4(h)16.2(i)0(s)20.9(t)0(o)20.7(g)0(r)20.9(a)0(m)18.5(s)-404.1(we)24.6(re)-395(ge)19.7(ne)19.7(ra)19.7(te)25.3(d)-404.3(u)0(s)21(i)0(n)20.7(g)-404.3(th)20.7(e)-399.7(F)0(l)20.9(o)0(w)20(-)]TJ
0 -1.1174 TD
[(Jo)21(V1)20(0)-323.6(S)0(o)21(f)0(t)20.7(w)0(a)18.8(r)0(e)-308.6(\()0(F)21(l)0(o)20.7(w)0(J)20.2(o)-323.6(L)15(L)0(C)18.6(,)-320.2(As)20.2(hl)20.7(an)19.7(d,)-309.8(OR)18.9(\).)]TJ
/F10 1 Tf
9.9626 0 0 9.9626 313.0582 312.8314 Tm
[(Trili)-9.4(neage)-341.3(differen)-7.9(tiation)-339.2(of)-333.6(MSC)-8(s)]TJ
/F4 1 Tf
9.843 0 0 9.843 323.0361 295.0298 Tm
[(Differentiation)-309.4(of)-301.7(the)-298.3(fMSCs)-300.8(and)-304(iMSCs)-301(into)-298.4(adipocytes,)]TJ
-1.0137 -1.1174 TD
[(chondrocytes,)-388.7(and)-384.6(osteoblasts)-388.6(was)-385.3(carried)-385.6(out)-383.6(for)-383.4(3)-381.2(weeks)]TJ
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[(using)-391.3(the)-390.5(STEMPRO)-392.7(Adipogenesis/Chondrogenesis/Osteo-)]TJ
T*
[(genesis)-747(Differentiation)-747.2(Kit)-741.7(\(Thermo)-748.5(Fisher)-739(Scientic\).)]TJ
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T*
[(Blue)-456(\(chondrocyte)-448.8(differentiation\),)-457.1(or)-451.4(2%)-446.8(Alizarin)-455.9(Red)-449(S)]TJ
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[(\(osteoblast)-429.1(differentiation\))-430.6(solutions)-424.6(according)-422.4(to)-422.8(standard)]TJ
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[(Cel)-8.6(l)-334(prolife)-8.4(ration)-340.2(analyses)]TJ
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[(The)-627.7(cell)-623.3(proliferation)-627.6(capacity)-630(of)-618.5(the)-626.6(fMSCs,)-626.8(iPSC-)]TJ
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[(iMSCs,)-269.8(and)-263.7(ESC-iMSCs)-272.9(was)-264.3(measured)-266.7(by)-267.1(the)-263.8(incorporation)]TJ
T*
[(of)-468.7(bromodeoxyuridine)-466(\(BrdU\))-469.2(using)-466.2(the)-465.4(cell)-467.8(proliferation)]TJ
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[(enzyme-lin)-10.2(ked)-396.1(immunosorbent)-403.9(assay)-404.9(\(ELISA\),)-400.1(BrdU)-403.7(\(col-)]TJ
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[(orimetric\))-325.9(kit)-320.3(\(Roche,)-324.4(Basel,)-323.3(Switzerland\),)-326.4(according)-318.8(to)-324.9(the)]TJ
T*
[(manufactur)-10(ers)-247.2(instructions.)-248.3(The)-247.6(cells)-249.8(were)-249.5(seeded)-251.8(at)-245.4(30,000)]TJ
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[(BrdU)-426.7(\(100)]TJ
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[(M\))-423.5(was)-425.6(added)-422.5(24)-163.5(h)-421.6(later)-428.5(for)-417.9(an)-423.8(incorporation)]TJ
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T*
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(2)Tj
45.3226 0 TD
[(SPITZHORN)-338.3(ET)-338.7(AL.)]TJ
ET
endstream
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T*
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T*
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T*
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T*
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T*
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[(On)6.5(e)-449.6(r)6.6(at)-442.8(\(T)5.4(7\))-442.7(t)6.4(h).8(at)-442.8(r)6.6(e)-.4(c)5.4(e)-.4(i)6.4(v).8(ed)-442.7(i)]TJ
12.176 0 TD
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[(PSC-i)5(MS)5.3(Cs)-443.9(sh)5.1(owed)-444.1(in)5.1(ternal)]TJ
-12.176 -1.1116 TD
.0128 Tc
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T*
.0106 Tc
[(w)-2.9(a)2.6(s,)-545.8(therefore,)-551.5(exclude)-3.2(d)-543.4(from)]TJ
13.7081 0 TD
.007 Tc
[(fu)-5.6(rt)-5.7(h)-5.6(e)-1(r)-552.8(a)-1(n)-5.6(a)-1(l)-5.7(y).1(s)-5.4(i)-5.7(s.)-555.1(Co)-5.6(nt)-5.7(ro)-5.6(l)]TJ
-13.7081 -1.1174 TD
.0121 Tc
[(Gu)5.2(nn)-501.6(rats)-501.4(un)5.2(derwent)-501.7(P)5.4(HX)-502.5(\()]TJ
/F5 1 Tf
12.4525 0 TD
0 Tc
(n)Tj
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(=)Tj
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.7315 0 TD
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[(4\))-5.4(,)-508.8(b).3(u)-5.4(t)-506.6(re)-6.6(ce)-6.6(i)-5.5(v).3(e)-6.6(d)-506.5(no)-506.5(c)-6.6(e)-.8(l)-5.5(l)]TJ
-13.8579 -1.1116 TD
.0118 Tc
[(trans)5.1(p)-.8(l)4.8(a)-2(nt)4.8(at)4.8(io)4.9(n.)-435.1(Aft)4.8(e)-2(r)-438.5(2)-438.6(mon)4.9(t)-.9(h)4.9(s)-438.4(of)-438.5(r)5(e)-2(covery)4.9(,)-440.9(t)4.8(he)-439.7(ani)4.8(m)-2(als)]TJ
T*
.0121 Tc
[(were)-479.7(s)5.4(a)-1.7(cri)5.1()-.4(ced)-478.6(b)5.2(y)-478.6(l)5.1(oss)-472.6(o)-.5(f)-478.5(b)5.2(lo)5.2(od)-472.8(dur)5.3(in)5.2(g)-478.6(a)4.1(nes)5.4(t)-.6(h)5.2(e)-1.7(s)5.4(i)-.6(a.)-475.1(The)]TJ
T*
[(livers)-230.6(w)-1.3(ere)-232(t)5.2(hen)-230.8(p)-.4(erfused)-230.8(with)-230.8(ph)5.3(ysio)5.3(logi)5.2(c)-232(buff)5.4(er)-230.8(and)-230.8(s)-.2(nap-)]TJ
0 -1.1174 TD
.0129 Tc
[(frozen)-454.7(in)-460.5(li)-5.6(qui)-5.6(d)-454.7(n)-5.5(it)-5.6(ro)-5.5(gen)-5.5(.)-457.1(Th)-5.5(e)-455.9(a)-.9(n)-5.5(i).2(ma)-6.6(l)-454.9(e)-6.6(xpe)-6.6(ri)-5.6(men)-5.5(ts)-460.3(we)-6.6(re)]TJ
0 -1.1116 TD
[(a)-6.5(p).4(p)-5.4(r).4(o)-5.4(v).4(ed)-276.1(by)-276.1(th)-5.4(e)-271.5(r)-5.3(el)-5.5(ev)-5.4(ant)-276.2(f).4(e)-6.5(d).4(e)-6.5(r).4(a)-6.5(l)-270.5(s)-5.2(t).3(a)-6.5(t).3(e)-277.2(a)-.8(ut)-5.5(ho)-5.4(ri)-5.5(ty)-276.1(fo)-5.4(r)-270.3(a)-6.5(nim)-6.6(a)-.8(l)]TJ
T*
.0206 Tc
[(protection)-735(\()2.3(Landesamt)-729.4(f)-3.5(u)]TJ
11.1162 .0058 TD
0 Tc
()Tj
.4377 -.0058 TD
.0205 Tc
[(r)-735.1(N)1.3(atur,)-731.7(U)-4.5(mwelt)-729.5(u)2.1(nd)-729.4(Ve)-4.8(r-)]TJ
-11.554 -1.1174 TD
.02 Tc
[(braucherschutz)-915.3(N)-5(o)1.6(rdrhein-We)-5.3(stfale)-5.3(n,)-910.7(R)-5.2(ecklinghausen,)]TJ
0 -1.1116 TD
.0188 Tc
[(G)-6.2(e)-.7(rma)-6.5(ny;)-333.8(R)-6.4(e)-.7(fer)-5.3(e)-.7(nc)-6.5(e)-334.8(N)-.4(o.)-336(84)-5.3(-02.0)-5.3(4).4(.201)-5.3(2.A34)-5.3(4).4(\).)]TJ
/F10 1 Tf
9.9626 0 0 9.9626 59.7543 107.3197 Tm
0 Tc
[(Ser)-8.2(um)-333.5(and)-335.7(cu)-6.4(lture)-334.5(su)-6.4(pernatant)-339.7(analysi)-9.3(s)]TJ
/F4 1 Tf
9.843 0 0 9.843 69.7323 89.4614 Tm
[(To)-256.8(determine)-262.5(the)-258(presence)-266.7(of)-255.6(albumin)-258(\(ALB\))-262(in)-261.5(the)-258(blood)]TJ
-1.0137 -1.1116 TD
[(of)-244.1(Gunn)-247.2(rats)-247.4(2)-243(months)-243.9(after)-249.8(transplantation)-245.1(of)-249.8(human)-244(iMSCs)]TJ
T*
[(and)-269.4(control)-268.3(animals,)-274(the)-269.5(blood)-264.8(was)-270.1(collected)-274.1(from)-264.7(the)-269.5(portal)]TJ
0 -1.1174 TD
[(vein)-247.6(and)-246.4(diluted)-245.4(1:10,000.)-245.1(Human)-244.9(serum)-249.6(samples)-247.2(from)-247.5(three)]TJ
25.5097 67.9242 TD
[(individuals)-462.9(were)-462.6(diluted)-464.3(1:1,000,000,)-463.8(whereas)-466.9(culture)-465.3(su-)]TJ
0 -1.1174 TD
[(pernatants)-360.1(from)-362.7(fMSCs)-358.4(and)-361.6(iMSCs)-364.3(were)-358.9(taken)-365.1(without)-360.3(di-)]TJ
0 -1.1116 TD
[(lution.)-338.7(These)-342.9(samples)-339.3(were)-341.6(analyzed)-341.9(by)-336.3(an)-343.2(ELISA)-336.9(specic)]TJ
T*
[(for)-498.6(human)-503.2(ALB)-507.5(according)-503.1(to)-503.4(the)-499.9(manufacturers)-504.9(recom-)]TJ
T*
[(mendations)-561.8(\(human)-567.7(ELISA)-561.5(quantitation)-569.1(set,)-560.8(#)-565.6(E80-129;)]TJ
0 -1.1174 TD
[(Bethyl)-521.7(Laboratories,)-528.2(Montgomery\).)-525.8(The)-524(concentration)-531.9(of)]TJ
0 -1.1116 TD
[(total)-369.9(ALB,)-372.7(lactate)-375.6(dehydrogenase)-370.1(\(LDH\),)-374.7(alanine)-374.3(transam-)]TJ
T*
[(inase)-261.2(\(ALT\),)-265.6(and)-263.6(aspartate)-268(transaminase)-266.6(\(AST\))-260.8(in)-261.5(the)-263.8(serum)]TJ
0 -1.1174 TD
[(was)-287.4(quantied)-294.6(by)-290.2(a)-290.2(Spotchem)-288.8(EZ)-292.6(SP-4430)-289.6(using)-287.7(diagnostic)]TJ
0 -1.1116 TD
[(test)-535.5(strips)-536.3(for)-533.1(multiparameter)-541.4(analysis)-535.1(\(#)-537.8(77182;)-530.9(Arkray)]TJ
T*
[(Europe,)-565.3(Amstelveen,)-567.3(Netherlands\).)-568.2(The)-558.6(concentration)-572.3(of)]TJ
0 -1.1174 TD
[(total)-346.9(bilirubin)-344.5(in)-347.9(the)-350.2(serum)-347.5(was)-345(measured)-353.1(using)-345.3(the)-344.4(colori-)]TJ
0 -1.1116 TD
[(metric)-471.2(Bilirubin)-472.2(Assay)-467.9(kit)-470.1(\()-81.7(MAK126;)-471.3(Sigma-Aldrich\).)-472.7(A)]TJ
T*
[(cytokine)-535.5(array)-531.9(was)-535(performed)-537.5(according)-537.6(to)-532.2(the)-534.5(protocol)]TJ
T*
[(from)-512.4(Proteome)-519.2(Proler)-515.5(Human)-515.6(Cytokine)-512.4(Array)-514.3(Panel)-514.7(A)]TJ
0 -1.1174 TD
[(\(R&D)-595(Systems\))-604(using)-592.9(0.4)-166.9(mL)-597.9(of)-595.4(Gunn)-598.5(rat)-597.8(serum.)-598.6(The)]TJ
0 -1.1116 TD
[(emitted)-305.4(chemiluminescence)-311.8(was)-298.9(analyzed)-307.3(by)-301.7(measuring)-307.1(the)]TJ
T*
[(pixel)-271.9(density)-279.6(of)-272.9(each)-278.6(spot)-275(on)-272.9(the)-275.3(array)-278.5(membrane,)-277.4(including)]TJ
0 -1.1174 TD
[(reference)-338.2(and)-332.8(negative)-340.9(control)-331.6(spots.)]TJ
/F10 1 Tf
9.9626 0 0 9.9626 310.8472 501.8455 Tm
[(Analysi)-9.1(s)-330.8(o)0(f)-339.2(bile)-337.9(acids)-335.5(and)-335.7(thei)-7.4(r)-332.8(conjuga)-10.3(tes)]TJ
/F4 1 Tf
9.843 0 0 9.843 320.8251 484.0439 Tm
[(Bile)-231.5(acids)-238.2(were)-238(analyzed)-238.2(by)-238.3(ultra-high)-237.1(performance)-236.8(liquid)]TJ
-1.0137 -1.1174 TD
[(tandem)-321.3(mass)-317.4(spectrometry)-323.2(\(UHPLC-MS/MS\))-324.9(essentially)-326.8(as)]TJ
0 -1.1116 TD
[(described)-485.6([28].)-480.1(The)-483.7(system)-486.8(consists)-481.9(of)-480.2(a)-486.1(UHPLC-I)-483.8(class)]TJ
T*
[(\(Waters,)-390(United)-389(Kingdom\))-391.2(coupled)-390.3(to)-388.2(a)-388.2(Xevo)-392.3(TQ-S)-386.4(triple)]TJ
0 -1.1174 TD
[(quadruple)-473.1(mass)-472.9(spectrometer)-479.9(\(Waters\).)-477.5(Electrospray)-478.7(ioni-)]TJ
0 -1.1116 TD
[(zation)-596.7(was)-604.2(performed)-600.9(in)-595.6(the)-597.8(negat)-8(ive)-597.8(ionization)-601.4(mode.)]TJ
T*
[(Chromatographic)-333.5(separation)-331.3(was)-333.5(performed)-330.2(on)-330.5(a)-330.6(BEH)-328.9(C18)]TJ
0 -1.1174 TD
[(column)-274.1(\(2.1)]TJ
/F9 1 Tf
5.034 0 TD
()Tj
/F4 1 Tf
.7142 0 TD
[(100)-170.3(mm,)-273(1.7)]TJ
/F3 1 Tf
5.1722 0 TD
(m)Tj
/F4 1 Tf
.5529 0 TD
[(m\).)-271.8(The)-270.6(mobile)-274.2(phase)-278.3(consisted)]TJ
-11.4733 -1.1116 TD
[(of)-272.9(water)-279.5(containing)-278.7(0.1%)-272.7(formic)-279.8(acid)-271.8(and)-275.2(5)-168.1(m)0(M)-275(ammonium)]TJ
T*
[(acetate)-537.9(\(Eluent)-534.4(A\))-532.9(and)-534.4(acetonitrile)-536.9(\(Eluent)-534.4(B\).)-536.5(Analytes)]TJ
T*
[(were)-445.3(separated)-452.2(by)-445.7(gradient)-449.1(elution.)-450.4(The)-449.2(injection)-450.5(volume)]TJ
0 -1.1174 TD
[(was)-252.8(5)]TJ
/F3 1 Tf
2.4652 0 TD
(m)Tj
/F4 1 Tf
.5529 0 TD
[(L)-250(and)-252.1(the)-252.3(column)-251(was)-252.8(maintained)-252.3(at)-251.2(40)]TJ
/F9 1 Tf
15.8046 0 TD
()Tj
/F4 1 Tf
.3917 0 TD
[(C.)-253.2(Detection)]TJ
-19.2144 -1.1116 TD
[(of)-428.4(the)-430.8(bile)-432(acids)-434(and)-430.7(their)-433.1(glycine)-430.8(and)-436.4(taurine)-430.7(conjugates)]TJ
T*
[(was)-442.9(performed)-439.6(in)-440.1(selected)-445.5(reaction)-444.5(monitoring)-444.6(mode.)-442.2(All)]TJ
0 -1.1174 TD
[(standards)-438.2(as)-440.9(well)-444.4(as)-435.1(the)-442.3(deuterated)-442.1(internal)-443.5(standard)-443(sub-)]TJ
0 -1.1116 TD
[(stance)-280.7(\(d4-CA,)-288.4(d4-GCA)-280.1(and)-280.9(d5-TCA\))-287.2(were)-284(purchased)-283.9(from)]TJ
T*
[(Sigma-Aldrich)-335.7(and)-332.8(Steraloids)-341.7(\(Newport\).)]TJ
/F10 1 Tf
9.9626 0 0 9.9626 310.8472 282.6141 Tm
[(Reverse)-338.9(trans)-7(criptase-)-8.7(PCR)]TJ
/F4 1 Tf
9.843 0 0 9.843 320.8251 264.8125 Tm
[(Human-specic)-372.2(primers)-372.8(\(Supplementary)-371.5(Table)-372(S1\))-366(were)]TJ
-1.0137 -1.1116 TD
[(generated)-371.8(to)-365.2(detect)-373.2(human)-365(mRNA)-371.3(in)-365.2(Gunn)-368.1(rat)-367.4(liver.)-367.4(Total)]TJ
0 -1.1174 TD
[(RNA)-253.7(was)-252.8(isolated)-253.2(from)-253.2(ve)-252(different)-254.3(liver)-248.8(pieces)-257.7(of)-249.8(each)-255.6(rat)]TJ
0 -1.1116 TD
[(using)-368.3(the)-373.2(RNeasy)-373.5(Mini)-374.2(kit)-372.2(\(Qiagen,)-371.5(Hilden,)-375.2(Germany\))-376(ac-)]TJ
T*
[(cording)-717.4(to)-722.3(the)-718.8(manufacturers)-723.8(recommendations.)-724(First-)]TJ
0 -1.1174 TD
[(strand)-480(cDNA)-486.2(was)-483.2(made)-486(from)-483.6(1)]TJ
/F3 1 Tf
13.8003 0 TD
(m)Tj
/F4 1 Tf
.5529 0 TD
[(g)-484.9(total)-479.4(RNA)-484.1(per)-482.5(20)]TJ
/F3 1 Tf
8.772 0 TD
(m)Tj
/F4 1 Tf
.5529 0 TD
(L)Tj
-23.6782 -1.1116 TD
[(reaction)-461.8(volume)-458.4(\(RevertAid)-464.7(H)-457(Minus)-461.4(First)-460.4(Strand)-457(cDNA)]TJ
T*
[(Synthesis)-437(Kit;)-437.6(Thermo)-442.1(Fisher)-439.5(Scientic\).)-444.2(A)-433.9(standard)-443(pro-)]TJ
0 -1.1174 TD
[(tocol)-444.7(using)-443.2(the)-442.3(2)]TJ
/F9 1 Tf
7.4012 0 TD
()Tj
/F4 1 Tf
.72 0 TD
[(PCR)-442.9(Master)-441.7(Mix)-447.8(\(Thermo)-443.2(Fisher)-445.2(Sci-)]TJ
-8.1212 -1.1116 TD
[(entic\),)-261.2(0.8)]TJ
/F3 1 Tf
4.769 0 TD
(m)Tj
/F4 1 Tf
.5529 0 TD
[(M)-255.4(primers,)-261.1(and)-257.9(5)]TJ
/F3 1 Tf
7.0844 0 TD
(m)Tj
/F4 1 Tf
.5529 0 TD
[(L)-255.7(template)-261.6(cDNA)-261.6(per)-257.9(25)]TJ
/F3 1 Tf
10.1659 0 TD
(m)Tj
/F4 1 Tf
.5529 0 TD
(L)Tj
-23.6782 -1.1116 TD
[(reaction)-363.9(volume)-360.5(was)-362.2(used)-362.5(to)-365.2(obtain)-359.4(PCR)-362.3(products)-367(by)-359.3(con-)]TJ
T*
[(ventional)-577.1(PCR.)-578.9(For)-573.3(quantitativ)-9.3(e)-572.5(real-time)-585.2(PCR)-575.4(\(qPCR\),)]TJ
0 -1.1174 TD
[(12.5)-168(ng)-370.8(cDNA)-382.6(per)-373(sample,)-376.4(0.6)]TJ
/F3 1 Tf
12.8096 0 TD
(m)Tj
/F4 1 Tf
.5529 0 TD
[(M)-376.4(primers,)-376.3(and)-378.8(the)-373.2(Power)]TJ
-13.3625 -1.1116 TD
[(Sybr)-534.1(Green)-532.8(Master)-533.9(Mix)-534.2(\(Life)-537.8(Technologies\))-537.4(were)-531.7(used.)]TJ
T*
(Universal)Tj
/F3 1 Tf
4.2737 0 TD
(b)Tj
/F4 1 Tf
.5529 0 TD
[(-actin)-383.5(primers)-378.6(for)-383.4(humans)-383.2(and)-378.8(rats)-379.9(served)-383.1(as)-377.6(a)]TJ
-4.8266 -1.1174 TD
[(reference)-280.6(gene)-277.4(for)-273.9(the)-275.3(normalization)-278.7(of)-272.9(values)-279.6(calculated)-282.2(by)]TJ
0 -1.1116 TD
[(the)-396.3(2)]TJ
/F9 1 Tf
7.3823 0 0 6.5614 331.7668 93.8268 Tm
(-)Tj
/F3 1 Tf
.5529 0 TD
(DD)Tj
/F4 1 Tf
1.3363 0 TD
(Ct)Tj
9.843 0 0 9.843 356.655 89.4614 Tm
[(method.)-404.2(Human)-400.4(samples)-402.7(of)-399.6(normal)-400.8(liver)-398.6(tissue)]TJ
-4.6538 -1.1116 TD
[(from)-264.8(patients)-264.8(with)-264.7(tumors)-266.9(were)-266.8(used)-264.6(as)-262.4(a)-267.2(reference)-269.1(to)-261.5(assess)]TJ
0 -1.1174 TD
[(the)-246.5(abundance)-256.5(of)-244.1(iMSC-derived)-253.7(cells)-255.6(within)-249.8(Gunn)-247.2(rat)-252.2(tissue.)]TJ
0 -1.1116 TD
[(A)-249.6(written)-252(consent)-251.9(was)-247.1(given)-254.5(by)-249.9(the)-246.5(patients)-253.2(and)-252.1(the)-246.5(usage)-255.3(of)]TJ
/F7 1 Tf
8.9663 0 0 8.9663 59.7543 749.7069 Tm
(HUMAN)Tj
6.4556 0 0 6.4556 95.9811 749.7069 Tm
(I)Tj
8.9663 0 0 8.9663 97.8519 749.7069 Tm
[(MSCs)-329.2(SUPPORT)-336.8(LIVER)-336.6(REGENERATION)-339.2(IN)-335.1(GUNN)-334(RATS)-22619.9(3)]TJ
ET
endstream
endobj
65 0 obj
<>stream
/GS1 gs
BT
/F4 1 Tf
9.843 0 0 9.843 62.022 725.1588 Tm
0 0 0 rg
0 Tc
0 Tw
[(liver)-323.7(samples)-327.8(for)-320(research)-324.2(was)-327.7(approved)-322.1(by)-324.7(the)-321.4(local)-324.9(ethics)]TJ
0 -1.1174 TD
[(committee)-471.3(of)-462.9(the)-459.6(Medical)-467.4(Faculty)-470.9(of)-462.9(the)-465.4(Heinrich)-467.3(Heine)]TJ
0 -1.1116 TD
[(University)-335.8(Du)]TJ
5.368 .0115 TD
()Tj
.4147 -.0115 TD
[(sseldorf)-338(\(Germany\).)]TJ
/F10 1 Tf
9.9626 0 0 9.9626 62.022 677.3101 Tm
[(Immuno)-10.5(histoche)-8.9(mistry)-339.6(and)-335.7(immunou)-11.6(orescence)]TJ
0 -1.2007 TD
[(analysi)-9.3(s)]TJ
/F4 1 Tf
9.843 0 0 9.843 72 647.5463 Tm
[(For)-550.3(immunohistochemistry,)-555.9(tissue)-554.8(sections)-558.2(were)-554.7(made)]TJ
-1.0137 -1.1116 TD
[(from)-379.9(frozen)-377.5(livers)-376.4(and)-378.8(xed)-374(with)-379.9(4%)-377.6(formalin)-374.3(for)-377.6(20)-169.2(min.)]TJ
0 -1.1174 TD
[(The)-454.9(xed)-454.7(sections)-460.3(were)-456.8(treated)-460.7(with)-454.8(0.1%)-451.3(Triton)-457.3(X-100,)]TJ
0 -1.1116 TD
[(washed)-349.4(in)-342.1(PBS,)-341.5(and)-344.3(endogenous)-347.2(peroxidases)-349.4(were)-347.4(blocked)]TJ
T*
[(\(dual)-335.1(endogenous)-341.5(enzyme)-333.9(block,)-338.5(#)-335.2(S2003;)-334.9(Dako,)-332.4(Glostrup,)]TJ
0 -1.1174 TD
[(Denmark\).)-368(Unspecic)-365.4(binding)-359.3(of)-359.3(antibodies)-364.9(was)-362.2(prevented)]TJ
0 -1.1116 TD
[(by)-330.5(the)-332.9(treatment)-331.8(with)-333.8(10%)-326.9(FBS)-332.3(in)-330.6(PBS)-326.6(supplemented)-334.8(with)]TJ
T*
[(0.1%)-393.7(saponin.)-392.4(A)-393.6(mouse)-393.6(antibody)-390.4(against)-396(the)-396.3(human)-388(cyto-)]TJ
0 -1.1174 TD
[(solic)-242.9(p)16.2(r)0(o)26.6(t)16.1(e)15(i)16.1(n)-225.7(S)0(t)26.6(e)15(m)15(1)16.2(21)-209.5(\(#)-215.3(Y)15.3(4)16.2(0)16.2(4)0(1)26.6(0)16.2(;)-225.9(T)15(a)15(k)16.2(ar)25.5(a)-226.9(B)15.2(i)16.1(o)-225.7(U)15.3(S)0(A)25.9(,)-222.3(M)15.3(o)0(u)26.6(n)16.2(t)16.1(a)0(i)25.3(n)]TJ
0 -1.1116 TD
-.0158 Tc
[(Vi)-15.8(e)9.5(w)-.5(,)-485.8(C)-.6(A\))-483.4(a)-15.8(n)9.6(d)-483.4(a)-.8(n)-15.8(t)10.7(i-mo)-15.8(u)10.8(s).6(e)-484.6(i)-15.8(m)9.4(m)-.8(unog)-15.8(l)10.7(obul)-15.8(i)10.5(n).4(s)-489(c)-.8(oupl)-15.8(e)9.5(d)-483.4(wi)-15.8(t)10.5(h)]TJ
T*
0 Tc
[(h)16.2(o)0(r)26.6(s)16.4(e)15(r)0(a)25.5(d)16.2(i)16.1(s)0(h)-364.9(p)16.2(e)15(r)0(o)26.6(x)16.2(i)16.1(d)0(a)25.4(s)16.4(e)-376.6(\()0(#)-365(A)15.3(P1)26.8(9)16.2(2)16.2(P)16.3(;)-381.4(M)15.3(e)15(r)16.2(ck)-366.2(M)15.3(i)16.1(ll)26.3(i)16.1(p)16.2(or)26.6(e)15(,)-377.8(D)15.3(a)15(rm)25.4(-)]TJ
0 -1.1174 TD
-.0158 Tc
[(st)-15.8(a)9.5(d).4(t,)-641.3(Ge)-15.8(r)9.7(many)-15.8(\))-628.5(were)-645.9(used)-644.7(for)-644.7(diamin)-15.8(o)10.8(ben)-15.8(z)9.6(idin)-15.8(e)-629.7(\().4(DA)-15.8(B)8.9(\))]TJ
0 -1.1116 TD
-.016 Tc
[(st)-16(a)9.3(i).1(ni)-16(n)10.5(g)-333.9(\(#)-333.9(K)-16(3)9.7(467)-16(1)10.6(1-2)-16(;)-323.6(D)-.7(ako)-16(\))10.6(.)-336.2(T)-1(he)-335(ce)-16(l)9.3(l)-334(nuc)-16(l)9.3(e)-1(i)-334(w)-16(e)8.6(r).2(e)-335(c)-1(o)-16(u)10.6(nter)-16(-)]TJ
T*
-.0158 Tc
[(st)-15.8(a)9.5(i).3(ned)-299.1(b).4(y)-293.4(d).4(il)-15.8(u)10.7(t).3(ed)-293.4(h)-15.8(e)9.6(matox)-15.8(y)10.8(lin)-293.4(s)-15.8(o)11(lut)-15.8(i)10.5(on)-293.4(\(1)-15.8(:)10.7(5).4(,)-295.7(#)-293.4(1.051)-15.8(7)10.8(5).4(.050)-15.8(0)10.8(;)]TJ
T*
0 Tc
[(Merck)-365.8(Millipore\).)-362.6(For)-360.2(the)-361.7(identication)-363.9(of)-359.3(liver)-364(cell)-364.1(types,)]TJ
0 -1.1174 TD
[(DAB-stained)-460.8(sections)-460.3(were)-456.8(treated)-454.9(with)-460.6(monoclonal)-452.6(anti-)]TJ
0 -1.1116 TD
[(bodies)-537.6(aga)11.7(ins)14(t)-531.1(CK1)12.1(8)-531(\()0(#)-526.3(B)0(M)13.1(227)14(5P;)-521.6(Acr)12(is,)-523.9(He)13(rfo)14(rd,)-524(Ger)12(-)]TJ
T*
[(man)11.6(y\))-416.9(or)-416.9(HNF)12.4(4)]TJ
/F3 1 Tf
6.687 0 TD
(a)Tj
/F4 1 Tf
.9734 0 TD
[(\(#)-416.9(311)14(3;)-417(Cel)11.6(l)-421.7(Sig)14(na)13.9(lin)13.7(g,)-419.2(Dan)11.9(ver)12.8(s,)-413.3(MA\))12.2(.)]TJ
-7.6604 -1.1174 TD
[(The)-230.3(pri)13.9(ma)12.7(ry)-232.5(ant)12.7(ibo)13.9(die)12.7(s)-231.3(wer)12(e)-238.4(the)12.7(n)-231.5(lab)12.7(ele)11.5(d)-237.2(b)0(y)-226.8(Cy3)12.9(-co)12.8(nju)13.9(gat)12.7(ed)]TJ
0 -1.1116 TD
[(sec)11.8(ond)14(ary)-424.9(ant)12.7(ibo)13.8(die)12.7(s)-432.9(dir)13.9(ect)11.5(ed)-429.6(aga)11.7(in)15(st)-434.1(mou)18.5(se)-435.1(or)-428.4(rab)12.8(bit)-424(im-)]TJ
T*
[(mun)12.7(ogl)13.8(obu)14(lin)19.5(s)-484.7(\()0(#)-480.2(AP1)13.3(92C)12.9(,)-481.5(#)-484.9(AP1)13.3(82C)12.9(;)-485(Mer)11.9(ck-)12.8(Mi)14(lli)13.6(por)14(e\).)]TJ
0 -1.1174 TD
[(Ant)13(ibo)13.9(die)12.7(s)-467.4(aga)11.7(ins)14(t)-467.8(Col)12.8(lag)12.7(en)-464.1(1)-467.6(\()0(#)-462.9(C24)12.9(56;)-458.5(Sig)14(ma)12.7(-Al)13(dri)13.9(ch\))12.8(,)]TJ
0 -1.1116 TD
[(Col)12.8(lag)12.7(en)-475.7(4)-479.1(\()0(#)-480.2(ab6)12.8(586)14(;)-479.3(Abc)11.9(am)12.7(,)-481.5(Cam)10.6(bri)13.9(dge)12.8(,)-481.5(Uni)13(ted)-471.1(Kin)13(g-)]TJ
T*
[(dom)12.7(\),)-453.8(OCT)10.9(4-A)-453.4(\(#)-457.2(C30)12.9(A3;)-453.6(Cel)11.7(l)-462(Sig)14(nal)12.7(in)15(g\),)-454.9(and)-453.7(PDG)12.4(FR)]TJ
/F3 1 Tf
23.7415 0 TD
(b)Tj
/F4 1 Tf
-23.7415 -1.1116 TD
-.0227 Tc
[(\(#)-231.1(31)5(69)5(S;)-237(Cel)4.9(l)-237(Si)4.9(gn)5(al)4.9(in)5(g)5(\))-236.9(were)-238.1(u)5(s)-.6(ed)-236.9(fo)5(r)-236.9(i)4.9(mmun)5(o)5.2(uo)5(rescen)5(ce)-238.1(of)]TJ
0 -1.1174 TD
0 Tc
[(li)20.6(ve)19.7(r)-260.3(s)0(e)19.9(c)0(t)19.5(i)0(o)20.7(n)0(s)-243.9(a)0(n)19.7(d)-254.5(cu)13.9(lt)20.6(ur)20.9(ed)-245.3(ce)18.5(ll)20.6(s.)-246.2(Th)19.6(e)-255.7(s)0(e)19.9(c)0(t)13.8(i)0(o)20.7(n)0(s)-243.9(w)0(e)18.8(r)0(e)-245.2()0(x)21(e)0(d)-251(w)0(i)19.8(t)0(h)]TJ
0 -1.1116 TD
[(ic)19.5(e-)19.7(co)13.9(ld)-267.3(m)15(e)0(t)13.8(h)0(a)19.7(n)0(o)20.8(l)0(,)-269.6(w)0(h)20(e)0(r)19.7(e)0(a)18.5(s)-277.4(4%)-268.2(PF)21.2(A)-278.4(w)0(a)18.8(s)-277.4(us)21(ed)-268.3(to)-267.3(x)-267(cu)19.7(lt)14.8(ur)20.9(ed)]TJ
T*
-.0281 Tc
[(cells)5.6(.)-325.3(A)-1.3(p)5.4(pro)5.4(pri)5.2(ate)-324.1(s)-.2(eco)5.4(ndary)-322.9(Cy3)5.4(-)-.4(,)-325.3(F)5.5(ITC-,)-325.3(o)-.4(r)-322.9(A)-1.3(l)5.2(e)-1.6(x)5.4(a)-329.9(Fl)5.2(uo)5.4(r)-328.7(5)-.4(5)5.4(5)-.4(-)]TJ
0 -1.1174 TD
-.0283 Tc
[(labeled)-236.7(a)-1.8(n)5.2(tib)5.2(odi)5(es)-236.6(\(#)-242.5(AP18)5.2(2C/F)]TJ
12.0551 0 TD
-.0287 Tc
[(,)-239.5(#)-237.1(AP1)4.8(92C/F)4.9(;)-243(M)-1.9(erck-Mil)4.6(lip)4.8(ore,)]TJ
-12.0551 -1.1116 TD
-.0203 Tc
[(#)-367(A)-20.3(-2)-20.3(14)-20.3(28)-20.3(;)-356.7(T)-20.3(he)-20.3(r)-6.3(m)-5.3(o)-367(F)-20.3(is)-20.3(h)-5(e)-20.3(r)-357.7(S)-20.3(ci)-20.3(en)-20.3(ti)-20.3(c)-20.3(\))-357.7(a)-20.3(nd)-367(4)]TJ
/F9 1 Tf
17.8666 0 TD
0 Tc
()Tj
/F4 1 Tf
.2419 0 TD
[(,6)18.5(-d)20.9(ia)19.6(mi)19.5(di)20.7(no)20.8(-2)15.1(-)]TJ
-18.1085 -1.1116 TD
[(ph)20.8(en)19.7(yl)15(in)20.7(do)20.8(le)-470(\(D)20(AP)20.1(I)-479.1(F)0(l)20.9(u)0(o)20.8(r)0(o)15.1(m)0(o)19.6(u)0(n)20.8(t)-479.3(G,)-466.2(#)-479.2(0)0(1)20.8(0)0(0)20.8(-)0(2)20.9(0)0(;)-468.9(S)0(o)21(u)0(t)20.7(h)0(e)13.9(r)0(n)]TJ
0 -1.1174 TD
[(Bi)19.7(ot)20.7(ec)12.8(h,)-361.6(Bi)19.7(rm)19.6(in)20.7(gh)20.8(am)18.5(,)-377.8(A)0(L)18.8(\))-369.7(or)-365(Ho)20(ec)12.8(hs)21(t)-375.6(3)0(3)20.8(2)0(5)20.8(8)-375.5(dy)20.8(e)-370.9(\()0(1)15.1(:)0(5)20.7(,)13.8(00)15.1(0;)]TJ
0 -1.1116 TD
[(Si)20.9(gm)19.6(a-)14(Al)19.8(dr)20.9(ic)19.5(h\))-267.1(we)18.8(re)-268.3(ap)19.7(pl)20.7(ie)19.6(d)-277.6(t)0(o)-261.5(m)0(a)18.4(r)0(k)-267.1(p)0(r)20.9(i)0(m)19.5(a)0(r)14(y)-271.8(an)19.7(ti)14.8(bo)20.8(di)20.7(es)-268.1(an)19.7(d)]TJ
T*
[(ce)18.5(ll)-313.5(nu)20.8(cl)13.8(ei)19.6(,)-320.2(r)0(e)19.7(s)0(p)15.3(e)0(c)18.5(t)0(i)20.6(v)0(e)19.7(l)0(y)20.7(.)]TJ
/F10 1 Tf
9.9626 0 0 9.9626 62.022 260.1637 Tm
[(Fluores)-7.9(cen)-6.4(ce)-330.8(in)-339.7(situ)-338.2(hybridizat)-9.9(ion)]TJ
/F4 1 Tf
9.843 0 0 9.843 72 242.3622 Tm
[(Fl)20.9(uo)20.8(re)19.7(sc)14.1(en)19.7(ce)-327.1(in)-330.6(si)15.1(tu)-324.9(hy)15.1(br)20.9(id)20.7(iz)19.6(at)19.5(io)20.7(n)-340.9(\()0(F)21(I)0(S)15.3(H)0(\))-325.6(w)0(a)13(s)-335(ca)18.5(rr)15.1(ie)19.6(d)-340.9(o)0(u)20.8(t)]TJ
-1.0137 -1.1116 TD
[(af)19.7(te)19.6(r)-496.4()0(x)21(a)0(t)19.5(i)0(o)20.7(n)-496.4(of)-480.2(li)20.6(ve)13.9(r)-490.6(c)0(r)19.7(y)0(o)20.8(s)0(e)14.1(c)0(t)19.5(i)0(o)20.7(n)0(s)-480.1(w)0(i)14.1(t)0(h)-480.4(C)0(a)18.6(r)0(n)20.9(o)0(y)20.8()0(s)-485.8(s)0(o)21(l)0(u)15(t)0(i)20.6(o)0(n)]TJ
0 -1.1174 TD
[(\(7)20.9(5%)-481.3(me)18.4(th)20.7(an)19.7(ol)-480.4(an)13.9(d)-484.9(2)0(5)15.1(%)-486(ac)12.8(et)19.5(ic)-481.5(ac)18.5(id)20.7(\))-490.6(f)0(o)20.9(l)0(l)20.6(o)0(w)20(e)0(d)-481.4(b)0(y)-480.3(p)0(e)19.7(p)0(s)15.3(i)0(n)]TJ
0 -1.1116 TD
[(di)20.7(ge)19.7(st)15.1(io)20.7(n)-352.4(\()0(0)20.9(.)0(0)18.5(5)-156.6(mg)19.6(/m)19.5(L)-353.6(p)0(e)19.7(p)0(s)21(i)0(n)-342.1(f)0(r)15.1(o)0(m)-343.2(R)0(o)19.8(c)0(h)19.7(e)-353.6(in)-342.1(0.)18.5(01)-146.2(N)-353.3(c)0(h)19.7(l)0(o)15(r)0(i)20.7(c)]TJ
T*
[(ac)18.5(id)20.7(\).)-367.4(Hu)20(ma)18.4(n-)20.9(sp)15.3(ec)18.5(i)20.9(c)-376.6(Y)-376.3(ch)19.7(ro)20.9(mo)19.6(so)21(me)-367.5(pr)15.1(ob)20.8(e)-376.6(\()0(#)20.9(D)0(-)20(0)0(3)20.8(2)0(4)20.8(-)0(1)15.1(0)0(0)20.8(-)]TJ
0 -1.1174 TD
[(OR)18.9(;)-318(M)0(e)18.8(t)0(a)19.5(S)0(y)21(s)0(t)20.9(e)0(m)18.5(s)0(,)-303.8(A)0(l)14.1(t)0(l)20.6(u)0()20.8(h)0(e)19.7(i)0(m)19.5(,)-314.5(Ge)18.8(rm)19.7(an)19.7(y\))-307.4(an)19.7(d)-317.9(r)0(a)19.7(t)0(-)20.7(s)0(p)21(e)0(c)18.5(i)0()20.9(c)-319(X)]TJ
0 -1.1116 TD
[(ch)19.7(ro)20.9(mo)19.6(so)15.3(me)-246.5(pr)20.9(ob)15.1(e)-255.7(\()0(#)15.1(I)0(D)20(R)0(F)20(1)0(0)20.8(6)0(7)20.8(;)-260.4(Em)18.4(pi)20.7(re)-251(Ge)18.8(no)20.8(mi)19.5(cs)19.9(,)-262.6(B)0(u)19.8(f)0(f)20.9(a)0(l)19.6(o)0(,)]TJ
T*
[(NY)19.1(\))-582.8(w)0(e)18.8(r)0(e)-573.5(d)0(i)14.9(s)0(s)21.2(o)0(l)20.7(v)0(e)19.7(d)-582.8(in)-572.5(a)-584(h)0(y)20.8(b)0(r)20.9(i)0(d)15(i)0(z)19.6(a)0(t)19.5(i)0(o)20.7(n)-582.8(so)21(lu)20.7(ti)20.6(on)-578.2(\(E)19.7(mp)19.6(ir)20.7(e)]TJ
0 -1.1174 TD
[(Ge)18.8(no)20.8(mi)19.5(cs)19.9(\))-536.7(a)0(n)19.7(d)-536.7(hy)20.8(br)20.9(id)15(iz)19.6(ed)-521.7(wi)14.1(th)-520.7(th)20.7(e)-537.9(s)0(e)19.9(c)0(t)19.5(i)0(o)15(n)0(s)-520.4(o)0(v)15.1(e)0(r)19.7(n)0(i)20.7(g)0(h)20.8(t)-536.9(at)]TJ
0 -1.1116 TD
(37)Tj
/F9 1 Tf
.9791 0 TD
()Tj
/F4 1 Tf
.3859 0 TD
[(C)-249.8(a)0(f)19.7(t)0(e)19.6(r)-248.7(an)-239.5(in)20.7(it)20.6(ia)19.6(l)-248.9(d)0(e)19.7(n)0(a)19.7(t)0(u)20.7(r)0(a)19.7(t)0(i)20.6(o)0(n)-238.3(a)0(t)-239.6(7)0(5)]TJ
/F9 1 Tf
13.7945 0 TD
()Tj
/F4 1 Tf
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[(C.)-236(Af)14.2(te)19.6(r)-243(w)0(a)18.8(s)0(h)15.3(i)0(n)20.7(g)-248.8(wi)19.8(th)]TJ
-15.5454 -1.1116 TD
.013 Tc
[(s)-5.2(a)-.8(li)-5.5(ne)-473.1(sod)-5.4(i).3(u)-5.4(m)-467.4(ci)-5.5(tr)-5.3(ate)-6.5(,)-468.5(the)-473.1(s).6(e)-6.5(c)-.8(ti)-5.5(on)-5.4(s)-466(w)-.5(e)-6.5(r).4(e)-473.1(c)-.8(ov)-5.4(er)-5.3(ed)-466.1(w)-6.2(i).3(t)-5.5(h)-466.2(a)]TJ
0 -1.1174 TD
-.0159 Tc
[(mou)-15.9(n)10.7(tin)-15.9(g)-265.8(medi)-15.9(u)10.6(m)-277.4(\().3(DA)-15.9(P)10(I)-276.1(F)-15.9(l)10.7(uor)-15.9(o)10.7(m)-.9(oun)-15.9(t)-265.9(G)-.6(;)-276.3(S)-15.9(o)10.9(u).3(th)-15.9(e)9.5(r).3(n)-276.2(B)-15.9(i)9.5(o).3(te)-15.9(c)8.4(h\).)]TJ
/F10 1 Tf
9.9626 0 0 9.9626 62.022 96.3779 Tm
0 Tc
[(Statist)-9.3(ics)]TJ
/F4 1 Tf
9.843 0 0 9.843 72 78.5763 Tm
[(All)-551.6(data)-554(were)-554.7(indicated)-555.2(as)-550.3(arithmetic)-557.6(mean)]TJ
/F9 1 Tf
19.1625 0 TD
()Tj
/F4 1 Tf
.72 0 TD
(standard)Tj
-20.8962 -1.1116 TD
[(error)-438.5(of)-434.1(mean.)-437.6(Statistical)-442.2(analysis)-443(was)-437.1(performed)-439.6(by)-434.2(Stu-)]TJ
0 -1.1174 TD
(dents)Tj
/F5 1 Tf
2.7128 0 TD
(t)Tj
/F4 1 Tf
.2822 0 TD
[(-test)-271.6(and)-263.7(ANOVA)-269.1(\(analysis)-271.3(of)-267.1(variance\).)]TJ
/F5 1 Tf
16.755 0 TD
(P)Tj
/F4 1 Tf
.8812 0 TD
[(values)-268.1(of)]TJ
/F9 1 Tf
4.8727 67.9184 TD
(<)Tj
/F4 1 Tf
.5529 0 TD
[(0.05)-277.4(were)-278.3(considered)-279.4(signicant)-278.3(and)-275.2(indicated)-278.7(by)-272.9(different)]TJ
-.5529 -1.1174 TD
[(letters)-338.5(in)-336.4(the)-332.9(graphs.)]TJ
/F7 1 Tf
9.9626 0 0 9.9626 313.0582 683.8865 Tm
[(Resu)-10.9(lts)]TJ
/F10 1 Tf
0 -1.673 TD
[(Hum)-8.2(an)-335.7(ESC-)-342.7(and)-335.7(iPSC-de)-10.9(rived)-337.4(iMSCs)]TJ
0 -1.2007 TD
[(are)-338.4(funct)-7.8(ional)-337.8(MSC)-8(s)]TJ
/F4 1 Tf
9.843 0 0 9.843 323.0361 637.3983 Tm
[(Hu)25.7(m)15(a)15(n)-317.9(i)16.1(M)15.3(SC)25.7(s)-311.9(w)15.3(er)25.5(e)-313.3(d)0(e)25.4(r)16.2(iv)26.5(e)15(d)-312.1(fr)26.7(om)-302.9(t)16.1(h)16.2(e)-313.3(E)0(S)25.6(C)-313.1(l)16.1(i)0(n)26.5(e)-313.3(H1)-302.6(\()16.2(E)15(SC)25.7(-)]TJ
-1.0137 -1.1116 TD
[(iM)25.6(SC)25.7(s)16.4(\))-266(an)25.4(d)-266(i)16.1(PS)26.9(C)15.2(s)-265.8(fr)26.7(om)-251.1(fe)25.5(t)16(a)0(l)-256.9(M)15.3(S)16.3(C)0(s)-256.5(\()16.2(i)16.1(P)0(S)26.9(C)15.2(-i)26.5(MS)25.9(C)15.2(s)0(\))-255.4(b)16.2(y)-266(i)16(nh)26.6(i)16(b)0(i)26.5(-)]TJ
T*
[(ti)26.3(on)-388.1(o)16.2(f)-404.2(T)15(G)15.3(F-)]TJ
/F3 1 Tf
5.3047 0 TD
(b)Tj
/F4 1 Tf
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0 -1.1116 TD
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T*
[(mo)25.4(r)16.2(p)0(h)26.6(o)0(l)26.5(o)16.2(gy)-388.1(a)15(s)-398.3(we)24.6(l)16.1(l)-398.6(as)-389.1(e)15(x)0(p)26.6(r)16.2(es)25.6(si)26.7(o)16.2(n)-398.5(of)-388.1(M)15.3(S)0(C)-389(s)16.4(u)16.2(r)0(f)26.7(a)0(c)24.3(e)-399.7(m)15(a)0(r)25.5(k)16.2(er)25.5(s)]TJ
0 -1.1174 TD
[(su)26.8(ch)-435.3(a)15(s)-450.2(P)16.3(D)0(G)24.9(F)0(R)]TJ
/F3 1 Tf
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[(\(F)26.8(ig)26.5(.)-446.9(1)16.2(A\))25.8(.)-446.9(T)0(h)25.4(e)-445.8(tr)26.5(an)25.4(s)16.4(c)0(r)25.5(i)0(p)26.5(t)16.1(om)25.4(e)15(s)-450.2(o)16.2(f)-450.3(i)16(M)15.3(SC)25.7(s)]TJ
-7.6431 -1.1116 TD
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T*
[(iP)26.6(SC)25.7(s)16.4(,)-176.2(ES)25.6(C)15.2(s)0(,)-165.6(a)15(n)16.2(d)-173.9(fM)25.7(SC)25.7(s)-173.7(b)0(y)-163.5(m)15(i)16.1(c)0(r)25.5(o)0(a)25.4(r)16.2(ra)25.5(y)-173.9(a)0(n)25.4(a)15(ly)26.5(si)26.7(s)-173.7(\()0(F)26.8(i)16.1(g.)-165.8(1)16.2(B)15.2(\).)-165.8(T)15(h)16.2(e)]TJ
0 -1.1174 TD
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0 -1.1116 TD
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[(CD)-15.2(1)10.5(0)1(5)]TJ
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[(CD)24.7(1)16.2(4)0(6)]TJ
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[(,a)314.4(n)339.8(d)]TJ
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(b)Tj
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/F5 1 Tf
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[(OCT)-15.3(4)]TJ
/F4 1 Tf
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-11.4273 -1.1174 TD
[(Y\))25.8(-b)26.6(o)16.2(x)-185.4(2)-185.4(\()]TJ
/F5 1 Tf
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[(SOX)-15.8(2)]TJ
/F4 1 Tf
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[(\),)-203.3(an)-15.6(d)]TJ
/F5 1 Tf
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[(N)15.2(A)15(NO)24.7(G)]TJ
/F4 1 Tf
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-11.9571 -1.1116 TD
0 Tc
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T*
[(th)26.5(is)26.7(,)-458.4(P)0(e)25.6(a)15(rs)26.8(on)-445.7(co)25.4(r)16.2(r)0(e)25.5(l)16.1(at)25.3(io)26.5(n)-456.1(a)0(n)25.4(a)0(l)25.3(y)16.2(si)26.7(s)-461.7(o)16.2(f)-461.8(t)16.1(he)-446.9(tr)26.5(a)15(n)0(s)26.8(c)15(ri)26.5(pt)26.5(o)16.2(m)0(e)-448.1(d)0(a)25.4(t)16(a)]TJ
0 -1.1174 TD
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0 -1.1116 TD
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/F5 1 Tf
14.0134 0 TD
(R)Tj
/F4 1 Tf
7.3823 0 0 6.5614 456.8881 444.529 Tm
(2)Tj
9.843 0 0 9.843 462.2739 440.1636 Tm
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-15.1595 -1.1116 TD
0 Tc
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0 -1.1174 TD
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0 -1.1116 TD
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T*
[(iM)25.6(SC)25.7(s)16.4(,)-251.1(an)25.4(d)-248.8(\()0(2)26.6(\))-254.5(E)15(S)16.3(C)-255.6(a)15(n)16.2(d)-254.5(E)15(S)16.3(C-)25.6(i)16.1(M)0(S)25.9(C)15.2(s,)-240.5(a)15(s)-254.3(i)16(n)16.2(di)26.5(c)15(a)0(t)25.3(e)15(d)-254.5(b)16.2(y)-254.5(d)16.2(i)16.1(s)0(t)26.7(i)0(n)26.5(c)15(t)]TJ
0 -1.1174 TD
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1.0137 -1.1116 TD
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-1.0137 -1.1116 TD
[(s)-15.7(u)11.1(rf)-15.7(a)9.8(c)-.7(e)-553.6(mar)-15.7(k)10.9(e)-.7(r)-558.2(e)-.7(xpre)-15.7(s)9.9(s).7(i)-15.7(o)10.8(n)-552.5(\().5(CD)-15.7(7)10(3).5(,)-554.8(C)-.5(D9)-15.7(0)10.9(,)-554.8(and)-558.2(C)-.5(D105)-15.7(\))-542(i).4(n)]TJ
T*
[(i)-15.7(M)9.9(SCs)-275.8(a)-15.7(n)9.7(d)-276(fMS)-15.7(C)10(s)-275.8(\().5(F)-15.7(i)10.9(g.)-278.3(2AI)-15.7(\))11(,)-278.3(and)-276(mar)-15.7(k)10.9(e)-.7(rs)-275.8(o)-15.7(f)-265.5(hemat)-15.7(o)10.8(poi)-15.7(e)9.6(t).4(ic)]TJ
0 -1.1174 TD
[(c)-15.7(e)8.6(lls)-333.4(w)-.4(er)-15.7(e)-318.5(a)-.7(bs)-15.7(e)9.9(n).5(t)-333.7(\().5(Fig)-15.7(.)-319.7(2JL)-15.7(\))9.7(.)-330.2(Sta)-15.7(t)9.6(ist)-15.7(i)10.6(cal)-333.7(anal)-15.7(y)10.8(s).7(is)-333.4(rev)-15.7(e)9.7(aled)-333.6(no)]TJ
0 -1.1116 TD
[(s)-15.7(i)11(gni)-15.7()10.9(can)-15.7(t)-409.7(d).5(i)-15.7(f)10.8(fer)-15.7(e)9.8(nce)-15.7(s)-410.5(be)-15.7(t)9.6(w)-.4(een)-425.7(iM)-15.7(S)10.2(C)-.5(s)-425.5(a)-.7(nd)-425.7(the)-426.9(f).5(MS)-15.7(C)10(s)-419.8(w)-15.7(i)9.9(th)]TJ
T*
0 Tc
[(re)25.5(s)16.4(p)16.2(ec)24.3(t)-266.2(t)16.1(o)-260.3(t)0(h)26.5(e)15(s)16.4(e)-267.2(c)15(e)15(l)0(l)-250.1(s)0(u)26.8(r)16.2(fa)25.5(c)15(e)-267.2(m)15(a)15(r)16.2(k)16.2(e)15(rs)26.8(.)-262.6(F)0(u)26.8(r)16.2(t)16.1(h)0(e)25.4(r)16.2(m)15(o)16.2(re)25.5(,)-262.6(t)16.1(he)-251(f)16.2(M)0(S)25.8(C)15.2(s)]TJ
0 -1.1174 TD
[(as)-164.4(w)15.3(e)15(ll)-163.7(a)15(s)-179.5(t)16.1(h)16.2(e)-175(i)0(M)25.6(S)16.3(C)15.2(s)-173.7(di)26.5(s)16.4(p)0(l)26.5(a)15(y)16.2(e)15(d)-179.6(a)-175(s)16.4(p)16.2(in)26.5(d)16.2(l)16.1(e-)25.5(s)16.4(h)16.2(ap)25.4(e)15(d)16.2(,)-176.2()16.3(br)26.6(o)16.2(b)16.2(l)0(a)25.3(s)16.4(t)16.1(-)0(l)26.5(i)16(k)16.2(e)]TJ
0 -1.1116 TD
-.016 Tc
[(m)-16(o)9.4(rpho)-16(l)10.5(ogy)-374.2(\()-16(F)10.8(ig.)-376.5(3).2(A1)-16()10.6(A)-.7(3\))-16(.)-360.3(S)-16(i)10.6(m)-1(ilar)-379.9(to)-374.2(fMSC)-16(s)9.8(,)-376.5(iMSCs)-379.8(from)]TJ
T*
.0127 Tc
[(E)12.7(S)38.3(C)27.9(s)-293.5(a)12.7(n)38.1(d)-293.7(i)28.8(P)12.7(S)39.6(C)27.8(s)-293.5(were)-317.9(observed)-316.7(t)0(o)-322.5(d).1(i)5.7(ffer)5.9(e)-1.1(nti)5.7(a)-1.1(te)-317.9(into)-316.7(adip)5.8(o-)]TJ
0 -1.1174 TD
.0121 Tc
[(cytes)-420.8(w)-1.4(ith)-421(char)5.3(acterist)5.1(i)-.6(c)-422.1(f)-.5(at)-421.1(drop)5.2(lets)-420.8(f)5.3(o)-.5(llo)5.2(wing)-421(t)5.1(r)-.5(eatment)]TJ
0 -1.1116 TD
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[(with)-270.2(an)-270.2(ad)6.2(ipoc)5.1(yte)-271.4(d).5(i)6.1(ffer)6.3(e)-.7(nti)6.1(a)-.7(tio)6.2(n)-276(m)5(edi)6.1(u).5(m,)-266.8(as)-270(evid)6.2(enc)5.1(e)-.7(d)-270.2(b).5(y)]TJ
T*
.0126 Tc
[(Oil)-236.3(R)-1(ed)-241.9(O)-237(s)5.9(tain)5.7(ing)-236.2(\()0(F)5.9(i)-.1(g.)-238.5(3)5.7(B)-1(1)5.7(B3\).)-232.7(A)-237(t)-.1(ypi)5.6(cal)-236.3(di)5.6(ffer)5.8(e)-1.2(nti)5.6(a)-1.2(ti)5.6(on)]TJ
0 -1.1174 TD
[(alon)5.6(g)-403.3(t)-.2(h)5.6(e)-404.5(ch)5.6(ond)5.6(rogenic)-398.7(a)-1.3(nd)-397.5(osteogenic)-398.7(cel)5.5(l)-403.4(lin)5.6(eag)5.6(es)-403.1(was)]TJ
0 -1.1116 TD
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[(demonst)5(r)-.6(ated)-259.8(for)-259.8(iMSCs,)-256.4(as)-259.6(seen)-259.8(in)-259.8(fMSCs)-259.6(\()-.6(F)5.3(i)-.8(g.)-262.1(3)5.1(C)-1.6(1)5.1(D3\).)]TJ
T*
.0123 Tc
[(A)-197(B)-1.3(rd)5.4(U)-197(E)-1.5(LISA)-197(was)-196(p)-.3(erfor)5.5(med)-190.4(to)-196.1(d)5.4(e)-1.5(ter)5.5(m)-1.5(in)5.4(e)-197.3(t)-.4(he)-191.5(rel)5.3(a)-1.5(tiv)5.4(e)-197.3(c)-1.5(ell)]TJ
T*
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