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3D human bone marrow stromal and endothelial cell spheres promote bone healing in an osteogenic niche

3D human bone marrow stromal and endothelial cell spheres promote bone healing in an osteogenic niche
3D human bone marrow stromal and endothelial cell spheres promote bone healing in an osteogenic niche
The current study used an ex vivo [embryonic day (E)18] chick femur defect model to examine the bone regenerative capacity of implanted 3-dimensional (3D) skeletal–endothelial cell constructs. Human bone marrow stromal cell (HBMSC) and HUVEC spheroids were implanted within a bone defect site to determine the osteogenic potential of the skeletal–endothelial cell unit. Cells were pelleted as co- or monocell spheroids and placed within 1-mm-drill defects in the mid-diaphysis of E18 chick femurs and cultured organotypically for 10 d. Micro-computed tomography analysis revealed significantly (P = 0.0001) increased levels of bone volume (BV) and BV/tissue volume ratio in all cell-pellet groups compared with the sham defect group. The highest increase was seen in BV in femurs containing the HUVEC and HBMSC monocell constructs. Type II collagen expression was particularly pronounced within the cell spheres containing HBMSCs and HUVECs, and CD31-positive cell clusters were prominent within HUVEC-implanted defects. These studies demonstrate the importance of the 3D osteogenic-endothelial niche interaction in bone regeneration. Elucidating the component cell interactions in the osteogenic-vascular niche and the role of exogenous factors in driving these osteogenic processes will aid the development of better bone reparative strategies.—Inglis, S., Kanczler, J. M., Oreffo, R. O. C. 3D human bone marrow stromal and endothelial cell spheres promote bone healing in an osteogenic niche.
0892-6638
Inglis, Stefanie
5cf406da-ae43-41c0-9088-cb812574f0bd
Kanczler, Janos
eb8db9ff-a038-475f-9030-48eef2b0559c
Oreffo, Richard
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
Inglis, Stefanie
5cf406da-ae43-41c0-9088-cb812574f0bd
Kanczler, Janos
eb8db9ff-a038-475f-9030-48eef2b0559c
Oreffo, Richard
ff9fff72-6855-4d0f-bfb2-311d0e8f3778

Inglis, Stefanie, Kanczler, Janos and Oreffo, Richard (2019) 3D human bone marrow stromal and endothelial cell spheres promote bone healing in an osteogenic niche. The FASEB Journal, 33. (doi:10.1096/fj.201801114R).

Record type: Article

Abstract

The current study used an ex vivo [embryonic day (E)18] chick femur defect model to examine the bone regenerative capacity of implanted 3-dimensional (3D) skeletal–endothelial cell constructs. Human bone marrow stromal cell (HBMSC) and HUVEC spheroids were implanted within a bone defect site to determine the osteogenic potential of the skeletal–endothelial cell unit. Cells were pelleted as co- or monocell spheroids and placed within 1-mm-drill defects in the mid-diaphysis of E18 chick femurs and cultured organotypically for 10 d. Micro-computed tomography analysis revealed significantly (P = 0.0001) increased levels of bone volume (BV) and BV/tissue volume ratio in all cell-pellet groups compared with the sham defect group. The highest increase was seen in BV in femurs containing the HUVEC and HBMSC monocell constructs. Type II collagen expression was particularly pronounced within the cell spheres containing HBMSCs and HUVECs, and CD31-positive cell clusters were prominent within HUVEC-implanted defects. These studies demonstrate the importance of the 3D osteogenic-endothelial niche interaction in bone regeneration. Elucidating the component cell interactions in the osteogenic-vascular niche and the role of exogenous factors in driving these osteogenic processes will aid the development of better bone reparative strategies.—Inglis, S., Kanczler, J. M., Oreffo, R. O. C. 3D human bone marrow stromal and endothelial cell spheres promote bone healing in an osteogenic niche.

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More information

Accepted/In Press date: 9 October 2018
e-pub ahead of print date: 7 November 2018
Published date: March 2019

Identifiers

Local EPrints ID: 427064
URI: https://eprints.soton.ac.uk/id/eprint/427064
ISSN: 0892-6638
PURE UUID: 0349ef04-ebff-430a-b064-cf8cf03a0905
ORCID for Janos Kanczler: ORCID iD orcid.org/0000-0001-7249-0414
ORCID for Richard Oreffo: ORCID iD orcid.org/0000-0001-5995-6726

Catalogue record

Date deposited: 21 Dec 2018 16:30
Last modified: 14 Mar 2019 01:48

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