READ ME File For Supplementary videos for "Collective cell behaviour in long-range mechanosensing of extracellular matrix dimensions"

Dataset DOI: 10.5258/SOTON/D0772 

ReadMe Author: Camelia Gratiela Muresan (Tusan), University of Southampton

This dataset supports the publication:
AUTHORS Camelia G. Tusan, Yu-Hin Man, Hoda Zarkoob, David A. Johnston, Orestis G. Andriotis, Philipp J. Thurner, Shoufeng Yang, Edward A. Sander, Eileen Gentleman, Bram G. Sengers, Nicholas D. Evans
TITLE   Collective cell behavior in mechanosensing of substrate thickness
JOURNAL Biophysical Journal
PAPER DOI IF KNOWN https://doi.org/10.1016/j.bpj.2018.03.037


This dataset contains: 12 videos 

Data supporting Camelia G. Muresan Tusan's PhD thesis: Collective cell behaviour in long-range mechanosensing of extracellular matrix dimensions

The videos are as follows:

Supplementary video S1. MG63 cell detachment. MG63 cells were cultured on 2 kPa Col I coated PA hydrogels. The cells and the microbeads were imaged before and after adding triton (0.1% in PBS). 
Supplementary video S2. MDCK cell migration. MDCK cells were cultured on 2 kPa Col I coated PA hydrogels. The cells and the microbeads were imaged for 2 h. 
Supplementary video S3. MG63 ingle cell-induced surface displacements in the hydrogels were independent of thickness. MG63 cells were cultured on 1 kPa, Fn coated PA hydrogel. The cells were imaged for 24 h.
Supplementary video S4. Colonies on thin hydrogels spread to a greater degree than those on thick materials. MG63 colonies were cultured on soft (1 kPa) Fn coated PA gels. At 2 days post-plating (0 h), the evolution of colonies was recorded for 4 days in a time lapse experiment. On both thin (20 µm) and thick (200 µm) hydrogel, the morphology of colonies was similar at 2 days (0 h), but at later time points, colonies on thin materials spread more than colonies on thick materials. Adapted from Tusan et al. (Tusan et al., 2018).
Supplementary video S5. Cells cultured on thin and soft substrates occasionally remained less associated with the colony, but do not migrate away from the colony and in the end regained contact. MG63 cells were cultured on thin (20 µm) and soft (1 kPa) Fn coated PA hydrogel. At 2 days post-plating (0 h), the evolution of the colony was recorded for 60 h in a time lapse experiment. Adapted from Tusan et al. (Tusan et al., 2018).
Supplementary video S6. Colony induced displacements in the hydrogels. On the top panel is presented the evolution of the MG63 colonies seeded on soft (1 kPa) thin or thick Fn coated PA gels starting with day 2 (0 h). On the bottom panel, it can be seen the displacement induced by the colonies as indicated by the fiducial fluorescent marker beads incorporated in hydrogels. Adapted from Tusan et al. (Tusan et al., 2018).
Supplementary video S7. Colony induced greater displacements in the thick hydrogels. Displacements on thin hydrogels were localised primarily to the regions occupied by cells, whereas on thick hydrogels, displacements extended well beyond the colony periphery. The magnitude of the displacements (see the colour bar) was significantly lower on thin hydrogels compared to thick. Adapted from Tusan et al. (Tusan et al., 2018).
Supplementary video S8. Tracking of gel displacement. Displacements were in general directed inward, radially towards the colony centre, while on thin gels displacements were less directional, with both inward and outward displacements. Adapted from Tusan et al. (Tusan et al., 2018).
Supplementary video S9. The hydrogel deformations induced by the colony consistent with a ‘pinching’ of the material. The ‘pinching’ (indicated by the red arrows) was evident visually on fiduciary bead images as an alignment of marker beads in a direction radial to the colony centre. Adapted from Tusan et al. (Tusan et al., 2018).
Supplementary video S10. HKCES colonies divide only on thin hydrogels. HKCES colonies were cultured on soft (1 kPa), thin (20 µm) and thick (200 µm) pepsin-digested coated PA gels. At 4 days post-plating (0 h), the evolution of colonies was recorded for 2 days in a time lapse experiment. 
Supplementary video S11. HKCES colony induced greater displacements in the thick hydrogels. HKCES induced surface displacement on thin hydrogels were localised primarily to the regions occupied by cells, whereas on thick hydrogels, displacements extended beyond the colony periphery. The magnitude of the displacements (see the colour bar) was significantly lower on thin hydrogels compared to thick.
Supplementary video S12. Tracking of gel HKCES induced surface displacement. Displacements were in general directed inward, radially towards the colony centre, while on thin gels displacements were less directional, with both inward and outward displacements.



Date of data collection: 06/10/2014-30/09/2018

Information about geographic location of data collection: University of Southampton, U.K.

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