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Ultrafast laser-scanning optical resolution photoacoustic microscopy at up to 2 million A-lines per second

Ultrafast laser-scanning optical resolution photoacoustic microscopy at up to 2 million A-lines per second
Ultrafast laser-scanning optical resolution photoacoustic microscopy at up to 2 million A-lines per second

The imaging speed of optical resolution photoacoustic microscopy (OR-PAM) using pulsed excitation is fundamentally limited by the range ambiguity condition, which defines the maximum laser pulse repetition frequency (PRF). To operate at this theoretical upper limit and maximize acquisition speed, a custom-built fiber laser capable of operating at a PRF of up to 2 MHz was combined with a fast laser scanning optical OR-PAM system based on a stationary fiber-optic ultrasound sensor. A large area (10 mm × 10 mm) of the mouse ear was imaged within 8 s, when acquiring 16 million A-lines and operating the laser at a PRF of 2 MHz. This corresponds to a factor of four improvement in imaging speed compared to the fastest OR-PAM system previously reported. The ability to operate at high-imaging frame rates also allows the capture of hemodynamic events such as blood flow. It is considered that this system offers opportunities for high throughput imaging and visualizing dynamic physiological events using OR-PAM.

fiber laser, microscopy, optical resolution photoacoustic microscopy
1083-3668
1-9
Allen, Thomas John
b133860c-b02d-457b-bf57-831dd8584136
Spurrell, Josh
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Berendt, Martin O.
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Ogunlade, Olumide
4dcdbcc6-1589-4a72-b4e1-b2fe2363dad8
Alam, Shaif U.
2b6bdbe5-ddcc-4a88-9057-299360b93435
Zhang, Edward Z.
d98bcd67-bc62-4315-8df3-de57a6fa975c
Richardson, David J.
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Beard, Paul C.
cd0d1cb3-0cbf-4669-b744-d0750a6d7ef2
Allen, Thomas John
b133860c-b02d-457b-bf57-831dd8584136
Spurrell, Josh
adfee145-3770-41b4-a434-a956d775dae0
Berendt, Martin O.
8bbeb612-7bcf-4d49-8448-b3c0ef3cc362
Ogunlade, Olumide
4dcdbcc6-1589-4a72-b4e1-b2fe2363dad8
Alam, Shaif U.
2b6bdbe5-ddcc-4a88-9057-299360b93435
Zhang, Edward Z.
d98bcd67-bc62-4315-8df3-de57a6fa975c
Richardson, David J.
ebfe1ff9-d0c2-4e52-b7ae-c1b13bccdef3
Beard, Paul C.
cd0d1cb3-0cbf-4669-b744-d0750a6d7ef2

Allen, Thomas John, Spurrell, Josh, Berendt, Martin O., Ogunlade, Olumide, Alam, Shaif U., Zhang, Edward Z., Richardson, David J. and Beard, Paul C. (2018) Ultrafast laser-scanning optical resolution photoacoustic microscopy at up to 2 million A-lines per second. Journal of Biomedical Optics, 23 (12), 1-9. (doi:10.1117/1.JBO.23.12.126502).

Record type: Article

Abstract

The imaging speed of optical resolution photoacoustic microscopy (OR-PAM) using pulsed excitation is fundamentally limited by the range ambiguity condition, which defines the maximum laser pulse repetition frequency (PRF). To operate at this theoretical upper limit and maximize acquisition speed, a custom-built fiber laser capable of operating at a PRF of up to 2 MHz was combined with a fast laser scanning optical OR-PAM system based on a stationary fiber-optic ultrasound sensor. A large area (10 mm × 10 mm) of the mouse ear was imaged within 8 s, when acquiring 16 million A-lines and operating the laser at a PRF of 2 MHz. This corresponds to a factor of four improvement in imaging speed compared to the fastest OR-PAM system previously reported. The ability to operate at high-imaging frame rates also allows the capture of hemodynamic events such as blood flow. It is considered that this system offers opportunities for high throughput imaging and visualizing dynamic physiological events using OR-PAM.

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More information

Accepted/In Press date: 26 October 2018
Published date: 6 December 2018
Keywords: fiber laser, microscopy, optical resolution photoacoustic microscopy

Identifiers

Local EPrints ID: 427226
URI: https://eprints.soton.ac.uk/id/eprint/427226
ISSN: 1083-3668
PURE UUID: 4b5d34fe-a2f3-4192-92fd-180f8396db86
ORCID for David J. Richardson: ORCID iD orcid.org/0000-0002-7751-1058

Catalogue record

Date deposited: 09 Jan 2019 17:30
Last modified: 14 Mar 2019 01:54

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