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Sonothrombolysis with magnetically targeted microbubbles

Sonothrombolysis with magnetically targeted microbubbles
Sonothrombolysis with magnetically targeted microbubbles
Microbubble-enhanced sonothrombolysis is a promising approach to increase the safety and efficacy of current pharmacological treatments for ischemic stroke. Maintaining therapeutic concentrations of microbubbles and drugs at the clot site however poses a challenge. The objective of this study was to investigate the efficacy of magnetic microbubble targeting upon clot lysis rates in vitro. Retracted whole porcine blood clots were placed in a flow phantom of a partially occluded middle cerebral artery. The clots were treated with a combination of tissue plasminogen activator (0.75µg/mL), magnetic microbubbles (~10^7 microbubbles/mL), and ultrasound (0.5MHz, 630kPa peak rarefactional pressure, 0.2Hz pulse repetition frequency, 2% duty cycle). Magnetic targeting was achieved using a single permanent magnet element (0.08-0.38T and 12-140T/m in the region of the clot). The change in clot diameter was measured optically over the course of the experiment. Magnetic targeting produced a three-fold average increase in lysis rates and linear correlation was observed between lysis rate and total energy of acoustic emissions.
0301-5629
1151-1163
de Saint Victor, Marie
b558a637-b7b6-4b5f-ad84-1a93f74a425e
Barnsley, Lester C.
da7b9324-6305-4b35-8e4e-0f4a6410aa25
Carugo, Dario
0a4be6cd-e309-4ed8-a620-20256ce01179
Owen, Joshua
4e7fc6bc-f2c5-4622-894f-9e811eca84cd
Coussios, Constantin
55b78f4c-f241-4676-87d5-9ea5fb295f4f
Stride, Eleanor
c0143e95-81fa-47c8-b9bc-5b4fc319bba6
de Saint Victor, Marie
b558a637-b7b6-4b5f-ad84-1a93f74a425e
Barnsley, Lester C.
da7b9324-6305-4b35-8e4e-0f4a6410aa25
Carugo, Dario
0a4be6cd-e309-4ed8-a620-20256ce01179
Owen, Joshua
4e7fc6bc-f2c5-4622-894f-9e811eca84cd
Coussios, Constantin
55b78f4c-f241-4676-87d5-9ea5fb295f4f
Stride, Eleanor
c0143e95-81fa-47c8-b9bc-5b4fc319bba6

de Saint Victor, Marie, Barnsley, Lester C., Carugo, Dario, Owen, Joshua, Coussios, Constantin and Stride, Eleanor (2019) Sonothrombolysis with magnetically targeted microbubbles. Ultrasound in Medicine & Biology, 45 (5), 1151-1163. (doi:10.1016/j.ultrasmedbio.2018.12.014).

Record type: Article

Abstract

Microbubble-enhanced sonothrombolysis is a promising approach to increase the safety and efficacy of current pharmacological treatments for ischemic stroke. Maintaining therapeutic concentrations of microbubbles and drugs at the clot site however poses a challenge. The objective of this study was to investigate the efficacy of magnetic microbubble targeting upon clot lysis rates in vitro. Retracted whole porcine blood clots were placed in a flow phantom of a partially occluded middle cerebral artery. The clots were treated with a combination of tissue plasminogen activator (0.75µg/mL), magnetic microbubbles (~10^7 microbubbles/mL), and ultrasound (0.5MHz, 630kPa peak rarefactional pressure, 0.2Hz pulse repetition frequency, 2% duty cycle). Magnetic targeting was achieved using a single permanent magnet element (0.08-0.38T and 12-140T/m in the region of the clot). The change in clot diameter was measured optically over the course of the experiment. Magnetic targeting produced a three-fold average increase in lysis rates and linear correlation was observed between lysis rate and total energy of acoustic emissions.

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UMB-D-18-00275R2-approved - Accepted Manuscript
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UMB-sonothombolysis-2019 - Version of Record
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More information

Accepted/In Press date: 19 December 2018
e-pub ahead of print date: 14 February 2019
Published date: May 2019

Identifiers

Local EPrints ID: 427413
URI: http://eprints.soton.ac.uk/id/eprint/427413
ISSN: 0301-5629
PURE UUID: 615be090-d3a6-48d4-816a-b5b94a0b42ce

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Date deposited: 15 Jan 2019 17:30
Last modified: 26 Nov 2021 07:10

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Contributors

Author: Marie de Saint Victor
Author: Lester C. Barnsley
Author: Dario Carugo
Author: Joshua Owen
Author: Constantin Coussios
Author: Eleanor Stride

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