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Generation of functional hepatocyte 3D discoids in an acoustofluidic bioreactor

Generation of functional hepatocyte 3D discoids in an acoustofluidic bioreactor
Generation of functional hepatocyte 3D discoids in an acoustofluidic bioreactor
Ultrasonic standing wave systems have previously been used for the generation of 3D constructs for a range of cell types. In the present study, we cultured cells from the human hepatoma Huh7 cell line in a Bulk Acoustic Wave (BAW) field and studied their viability, their functions and their response to the anti-cancer drug, 5 Fluorouracil (5FU). We found that cells grown in the acoustofluidic bioreactor (AFB) expressed no reduction in viability up to 6 h of exposure compared to those cultured in a conventional 2D system. In addition, constructs created in the AFB and subsequently cultured outside of it had improved functionality including higher albumin and urea production than 2D or pellet cultures. The viability of Huh7 cells grown in the ultrasound field to 5FU anti-cancer drug was comparable to that of cells cultured in the 2D system, showing rapid diffusion into the aggregate core. We have shown that AFB formed 3D cell constructs have improved functionality over conventional 2D monolayer and could be a promising model for anti-cancer drug testing.
1932-1058
1-12
Khedr, Mogib
37c876ff-7226-4d51-bd20-dfe0bbca3d73
Messaoudi, Walid, Mohamed
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Jonnalagadda, Umesh
8481bb68-d50f-4274-8e0f-4c1dc02bb3d7
Abdelmotelb, Ahmed
cebf8ef6-9dbe-433d-b8ac-f9f8c0ee3f94
Glynne-Jones, Peter
6ca3fcbc-14db-4af9-83e2-cf7c8b91ef0d
Hill, Martyn
0cda65c8-a70f-476f-b126-d2c4460a253e
Khakoo, Salim I
6c16d2f5-ae80-4d9b-9100-6bfb34ad0273
Abu Hilal, Mohammad
384e1c60-8519-4eed-8e92-91775aad4c47
Khedr, Mogib
37c876ff-7226-4d51-bd20-dfe0bbca3d73
Messaoudi, Walid, Mohamed
d66ce719-d255-4197-b11c-39886853669a
Jonnalagadda, Umesh
8481bb68-d50f-4274-8e0f-4c1dc02bb3d7
Abdelmotelb, Ahmed
cebf8ef6-9dbe-433d-b8ac-f9f8c0ee3f94
Glynne-Jones, Peter
6ca3fcbc-14db-4af9-83e2-cf7c8b91ef0d
Hill, Martyn
0cda65c8-a70f-476f-b126-d2c4460a253e
Khakoo, Salim I
6c16d2f5-ae80-4d9b-9100-6bfb34ad0273
Abu Hilal, Mohammad
384e1c60-8519-4eed-8e92-91775aad4c47

Khedr, Mogib, Messaoudi, Walid, Mohamed, Jonnalagadda, Umesh, Abdelmotelb, Ahmed, Glynne-Jones, Peter, Hill, Martyn, Khakoo, Salim I and Abu Hilal, Mohammad (2019) Generation of functional hepatocyte 3D discoids in an acoustofluidic bioreactor. Biomicrofluidics, 13 (1), 1-12, [014112]. (doi:10.1063/1.5082603).

Record type: Article

Abstract

Ultrasonic standing wave systems have previously been used for the generation of 3D constructs for a range of cell types. In the present study, we cultured cells from the human hepatoma Huh7 cell line in a Bulk Acoustic Wave (BAW) field and studied their viability, their functions and their response to the anti-cancer drug, 5 Fluorouracil (5FU). We found that cells grown in the acoustofluidic bioreactor (AFB) expressed no reduction in viability up to 6 h of exposure compared to those cultured in a conventional 2D system. In addition, constructs created in the AFB and subsequently cultured outside of it had improved functionality including higher albumin and urea production than 2D or pellet cultures. The viability of Huh7 cells grown in the ultrasound field to 5FU anti-cancer drug was comparable to that of cells cultured in the 2D system, showing rapid diffusion into the aggregate core. We have shown that AFB formed 3D cell constructs have improved functionality over conventional 2D monolayer and could be a promising model for anti-cancer drug testing.

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BIOMGB vol 13 iss 1014112 1 am - Accepted Manuscript
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Accepted/In Press date: 31 January 2019
e-pub ahead of print date: 12 February 2019

Identifiers

Local EPrints ID: 427914
URI: http://eprints.soton.ac.uk/id/eprint/427914
ISSN: 1932-1058
PURE UUID: e3f52615-2bad-4352-8f33-0aa3e1464c34
ORCID for Mogib Khedr: ORCID iD orcid.org/0000-0001-9942-4409
ORCID for Peter Glynne-Jones: ORCID iD orcid.org/0000-0001-5684-3953
ORCID for Martyn Hill: ORCID iD orcid.org/0000-0001-6448-9448

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Date deposited: 04 Feb 2019 17:30
Last modified: 26 Nov 2021 02:59

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Contributors

Author: Mogib Khedr ORCID iD
Author: Walid, Mohamed Messaoudi
Author: Ahmed Abdelmotelb
Author: Martyn Hill ORCID iD
Author: Salim I Khakoo
Author: Mohammad Abu Hilal

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