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Biomimetic oyster shell–replicated topography alters the behaviour of human skeletal stem cells

Biomimetic oyster shell–replicated topography alters the behaviour of human skeletal stem cells
Biomimetic oyster shell–replicated topography alters the behaviour of human skeletal stem cells

The regenerative potential of skeletal stem cells provides an attractive prospect to generate bone tissue needed for musculoskeletal reparation. A central issue remains efficacious, controlled cell differentiation strategies to aid progression of cell therapies to the clinic. The nacre surface from Pinctada maxima shells is known to enhance bone formation. However, to date, there is a paucity of information on the role of the topography of P. maxima surfaces, nacre and prism. To investigate this, nacre and prism topographical features were replicated onto polycaprolactone and skeletal stem cell behaviour on the surfaces studied. Skeletal stem cells on nacre surfaces exhibited an increase in cell area, increase in expression of osteogenic markers ALP (p < 0.05) and OCN (p < 0.01) and increased metabolite intensity (p < 0.05), indicating a role of nacre surface to induce osteogenic differentiation, while on prism surfaces, skeletal stem cells did not show alterations in cell area or osteogenic marker expression and a decrease in metabolite intensity (p < 0.05), demonstrating a distinct role for the prism surface, with the potential to maintain the skeletal stem cell phenotype.

bone regeneration, Nacre, osteogenic differentiation, skeletal stem cell, topography
2041-7314
1-13
Waddell, Shona J.
1b23df8b-b94a-44f7-8c2b-60d7e4c489aa
de Andrés, María C.
54e87e8a-1aa2-4907-a8a0-25d0c15e5e40
Tsimbouri, Penelope M.
7c504037-b1f7-4a9f-827d-fc55da742b64
Alakpa, Enateri V.
f749aaf8-6ac9-4215-84d5-34429bb220ac
Cusack, Maggie
365789bf-5513-40ed-952c-6c20fcff0fd6
Dalby, Matthew J.
25dcae6a-8289-4169-abb7-c45fff0bafdc
Oreffo, Richard O.C.
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
Waddell, Shona J.
1b23df8b-b94a-44f7-8c2b-60d7e4c489aa
de Andrés, María C.
54e87e8a-1aa2-4907-a8a0-25d0c15e5e40
Tsimbouri, Penelope M.
7c504037-b1f7-4a9f-827d-fc55da742b64
Alakpa, Enateri V.
f749aaf8-6ac9-4215-84d5-34429bb220ac
Cusack, Maggie
365789bf-5513-40ed-952c-6c20fcff0fd6
Dalby, Matthew J.
25dcae6a-8289-4169-abb7-c45fff0bafdc
Oreffo, Richard O.C.
ff9fff72-6855-4d0f-bfb2-311d0e8f3778

Waddell, Shona J., de Andrés, María C., Tsimbouri, Penelope M., Alakpa, Enateri V., Cusack, Maggie, Dalby, Matthew J. and Oreffo, Richard O.C. (2018) Biomimetic oyster shell–replicated topography alters the behaviour of human skeletal stem cells. Journal of Tissue Engineering, 9, 1-13. (doi:10.1177/2041731418794007).

Record type: Article

Abstract

The regenerative potential of skeletal stem cells provides an attractive prospect to generate bone tissue needed for musculoskeletal reparation. A central issue remains efficacious, controlled cell differentiation strategies to aid progression of cell therapies to the clinic. The nacre surface from Pinctada maxima shells is known to enhance bone formation. However, to date, there is a paucity of information on the role of the topography of P. maxima surfaces, nacre and prism. To investigate this, nacre and prism topographical features were replicated onto polycaprolactone and skeletal stem cell behaviour on the surfaces studied. Skeletal stem cells on nacre surfaces exhibited an increase in cell area, increase in expression of osteogenic markers ALP (p < 0.05) and OCN (p < 0.01) and increased metabolite intensity (p < 0.05), indicating a role of nacre surface to induce osteogenic differentiation, while on prism surfaces, skeletal stem cells did not show alterations in cell area or osteogenic marker expression and a decrease in metabolite intensity (p < 0.05), demonstrating a distinct role for the prism surface, with the potential to maintain the skeletal stem cell phenotype.

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More information

Accepted/In Press date: 19 July 2018
e-pub ahead of print date: 4 September 2018
Keywords: bone regeneration, Nacre, osteogenic differentiation, skeletal stem cell, topography

Identifiers

Local EPrints ID: 428024
URI: http://eprints.soton.ac.uk/id/eprint/428024
ISSN: 2041-7314
PURE UUID: ec67ce76-b431-442e-8539-973e7219b34a
ORCID for Richard O.C. Oreffo: ORCID iD orcid.org/0000-0001-5995-6726

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Date deposited: 07 Feb 2019 17:30
Last modified: 07 Oct 2020 01:44

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