Combined ex vivo 9.4T MRI and quantitative histopathological study in normal and pathological neocortical resections in focal epilepsy
Combined ex vivo 9.4T MRI and quantitative histopathological study in normal and pathological neocortical resections in focal epilepsy
High-resolution magnetic resonance imaging (MRI) may improve the preoperative diagnosis of focal cortical dysplasia (FCD) in epilepsy. Quantitative 9.4T MRI was carried out (T1, T2, T2* and magnetization transfer ratio) on 13 cortical resections, representing pathologically confirmed FCD (five cases) and normal cortex. Quantitative immunohistochemistry for myelination (myelin basic protein/SMI94), neuronal populations [microtubule-associated protein 2 (MAP2), neurofilament (SMI31, SMI32), synaptophysin, NeuN, calbindin], reactive glia (GFAP), microglia (CD68) and blood-brain barrier permeability (albumin) was carried out in 43 regions of interest (ROI) from normal and abnormal white matter and cortex. MRI was spatially aligned and quantitative analysis carried out on corresponding ROI. Line profile analysis (LPA) of intensity gradients through the cortex was carried out on MRI and immunostained sections. An inverse correlation was noted between myelin/SMI94 and T1, T2 (P < 0.005) and T2* (P < 0.05; Spearman's correlation) and a positive correlation between neuronal MAP2 and T1 (P < 0.005) and T2* (P < 0.05) over all ROI. Similar pathology-MRI correlations were observed for histologically unremarkable white matter ROI only. LPA showed altered gradient contours in regions of FCD, reflecting abnormal cortical lamination and myelo-architecture, including a preoperatively undetected FCD case. This study demonstrates the ability of quantitative 9.4T MRI to detect subtle differences in neuronal numbers and myelination in histologically normal appearing white matter and LPA in the evaluation of cortical dyslamination. These methods may be translatable to the in vivo detection of mild cortical malformations.
Adult, Epilepsies, Partial, Female, Humans, Image Processing, Computer-Assisted, Magnetic Resonance Imaging, Male, Middle Aged, Neocortex, White Matter, Young Adult, Journal Article, Research Support, Non-U.S. Gov't
319-333
Reeves, Cheryl
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Tachrount, Mohamed
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Thomas, David
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Michalak, Zuzanna
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Liu, Joan
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Ellis, Matthew
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Diehl, Beate
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Miserocchi, Anna
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McEvoy, Andrew W
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Eriksson, Sofia
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Yousry, Tarek
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Thom, Maria
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13 August 2016
Reeves, Cheryl
6912b99a-1a85-4ad3-860f-de808d19aca9
Tachrount, Mohamed
911a1fcf-15c1-4f5b-8c8b-b4a26e528d3a
Thomas, David
997c78e0-3849-4ce8-b1bc-86ebbdee3953
Michalak, Zuzanna
6396a6ff-f48c-4fef-a528-41420a0c0df5
Liu, Joan
0e68a532-ba7f-451a-944c-461e1585c47e
Ellis, Matthew
afbca752-ced4-40dd-b0af-d9ecffbd5b63
Diehl, Beate
f96b1f25-0ddc-430c-a707-644daba0822f
Miserocchi, Anna
6dc25cf8-a6ee-4460-b963-9ef4e469e0aa
McEvoy, Andrew W
5bdd5276-6522-4f4f-a39d-9983ca891c7c
Eriksson, Sofia
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Yousry, Tarek
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Thom, Maria
a9f63dd8-ba68-4ea1-a945-e4bbd18525d2
Reeves, Cheryl, Tachrount, Mohamed, Thomas, David, Michalak, Zuzanna, Liu, Joan, Ellis, Matthew, Diehl, Beate, Miserocchi, Anna, McEvoy, Andrew W, Eriksson, Sofia, Yousry, Tarek and Thom, Maria
(2016)
Combined ex vivo 9.4T MRI and quantitative histopathological study in normal and pathological neocortical resections in focal epilepsy.
Brain Pathology, 26 (3), .
(doi:10.1111/bpa.12298).
Abstract
High-resolution magnetic resonance imaging (MRI) may improve the preoperative diagnosis of focal cortical dysplasia (FCD) in epilepsy. Quantitative 9.4T MRI was carried out (T1, T2, T2* and magnetization transfer ratio) on 13 cortical resections, representing pathologically confirmed FCD (five cases) and normal cortex. Quantitative immunohistochemistry for myelination (myelin basic protein/SMI94), neuronal populations [microtubule-associated protein 2 (MAP2), neurofilament (SMI31, SMI32), synaptophysin, NeuN, calbindin], reactive glia (GFAP), microglia (CD68) and blood-brain barrier permeability (albumin) was carried out in 43 regions of interest (ROI) from normal and abnormal white matter and cortex. MRI was spatially aligned and quantitative analysis carried out on corresponding ROI. Line profile analysis (LPA) of intensity gradients through the cortex was carried out on MRI and immunostained sections. An inverse correlation was noted between myelin/SMI94 and T1, T2 (P < 0.005) and T2* (P < 0.05; Spearman's correlation) and a positive correlation between neuronal MAP2 and T1 (P < 0.005) and T2* (P < 0.05) over all ROI. Similar pathology-MRI correlations were observed for histologically unremarkable white matter ROI only. LPA showed altered gradient contours in regions of FCD, reflecting abnormal cortical lamination and myelo-architecture, including a preoperatively undetected FCD case. This study demonstrates the ability of quantitative 9.4T MRI to detect subtle differences in neuronal numbers and myelination in histologically normal appearing white matter and LPA in the evaluation of cortical dyslamination. These methods may be translatable to the in vivo detection of mild cortical malformations.
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Reeves_et_al-2016-Brain_Pathology
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Published date: 13 August 2016
Keywords:
Adult, Epilepsies, Partial, Female, Humans, Image Processing, Computer-Assisted, Magnetic Resonance Imaging, Male, Middle Aged, Neocortex, White Matter, Young Adult, Journal Article, Research Support, Non-U.S. Gov't
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Local EPrints ID: 428107
URI: http://eprints.soton.ac.uk/id/eprint/428107
ISSN: 1015-6305
PURE UUID: ca9de4c7-f2e1-487b-b5ca-c9f85ea34f04
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Date deposited: 11 Feb 2019 17:30
Last modified: 16 Mar 2024 00:09
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Author:
Cheryl Reeves
Author:
Mohamed Tachrount
Author:
Zuzanna Michalak
Author:
Joan Liu
Author:
Matthew Ellis
Author:
Beate Diehl
Author:
Anna Miserocchi
Author:
Andrew W McEvoy
Author:
Sofia Eriksson
Author:
Tarek Yousry
Author:
Maria Thom
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