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Isolation, differentiation, and characterization of human bone marrow stem cells In vitro and in vivo

Isolation, differentiation, and characterization of human bone marrow stem cells In vitro and in vivo
Isolation, differentiation, and characterization of human bone marrow stem cells In vitro and in vivo

In this chapter, we describe techniques for the isolation and characterisation of skeletal stem cells from human bone marrow. The methods for enrichment of STRO-1+ and STRO-4+ cells using magnetic activated cell sorting are described and we also detail techniques for establishing and characterizing osteogenic, adipogenic, and chondrogenic cultures from these cells. Finally, we present methods for studying the ability of these cells to produce bone in vivo using diffusion chambers which have been implanted subcutaneously into mice.

Adipocyte, Bone marrow, Chondrocyte, Diffusion chamber, Osteoblast, Stem cells
53-70
Humana
Kanczler, Janos
eb8db9ff-a038-475f-9030-48eef2b0559c
Tare, Rahul S.
587c9db4-e409-4e7c-a02a-677547ab724a
Stumpf, Patrick
dfdb286c-b321-46d3-8ba2-85b3b4a7f092
Noble, Timothy J.
a40ab178-af61-421e-adef-b4270c4a2b85
Black, Cameron
80d8f092-7395-4e6f-967b-c828502904e3
Oreffo, Richard O.C.
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
Idris, I.
Kanczler, Janos
eb8db9ff-a038-475f-9030-48eef2b0559c
Tare, Rahul S.
587c9db4-e409-4e7c-a02a-677547ab724a
Stumpf, Patrick
dfdb286c-b321-46d3-8ba2-85b3b4a7f092
Noble, Timothy J.
a40ab178-af61-421e-adef-b4270c4a2b85
Black, Cameron
80d8f092-7395-4e6f-967b-c828502904e3
Oreffo, Richard O.C.
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
Idris, I.

Kanczler, Janos, Tare, Rahul S., Stumpf, Patrick, Noble, Timothy J., Black, Cameron and Oreffo, Richard O.C. (2019) Isolation, differentiation, and characterization of human bone marrow stem cells In vitro and in vivo. In, Idris, I. (ed.) Bone Research Protocols. (Methods in Molecular Biology, , (doi:10.1007/978-1-4939-8997-3_4), 1914) New York. Humana, pp. 53-70. (doi:10.1007/978-1-4939-8997-3_4).

Record type: Book Section

Abstract

In this chapter, we describe techniques for the isolation and characterisation of skeletal stem cells from human bone marrow. The methods for enrichment of STRO-1+ and STRO-4+ cells using magnetic activated cell sorting are described and we also detail techniques for establishing and characterizing osteogenic, adipogenic, and chondrogenic cultures from these cells. Finally, we present methods for studying the ability of these cells to produce bone in vivo using diffusion chambers which have been implanted subcutaneously into mice.

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e-pub ahead of print date: 8 February 2019
Keywords: Adipocyte, Bone marrow, Chondrocyte, Diffusion chamber, Osteoblast, Stem cells

Identifiers

Local EPrints ID: 428370
URI: https://eprints.soton.ac.uk/id/eprint/428370
PURE UUID: 1c99d693-a4c3-414f-91f6-ad8d760513db
ORCID for Janos Kanczler: ORCID iD orcid.org/0000-0001-7249-0414
ORCID for Rahul S. Tare: ORCID iD orcid.org/0000-0001-8274-8837
ORCID for Patrick Stumpf: ORCID iD orcid.org/0000-0003-0862-0290
ORCID for Richard O.C. Oreffo: ORCID iD orcid.org/0000-0001-5995-6726

Catalogue record

Date deposited: 22 Feb 2019 17:30
Last modified: 20 Jul 2019 01:09

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Contributors

Author: Janos Kanczler ORCID iD
Author: Rahul S. Tare ORCID iD
Author: Patrick Stumpf ORCID iD
Author: Timothy J. Noble
Author: Cameron Black
Editor: I. Idris

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