Isolation, differentiation, and characterization of human bone marrow stem cells In vitro and in vivo
Isolation, differentiation, and characterization of human bone marrow stem cells In vitro and in vivo
In this chapter, we describe techniques for the isolation and characterisation of skeletal stem cells from human bone marrow. The methods for enrichment of STRO-1+ and STRO-4+ cells using magnetic activated cell sorting are described and we also detail techniques for establishing and characterizing osteogenic, adipogenic, and chondrogenic cultures from these cells. Finally, we present methods for studying the ability of these cells to produce bone in vivo using diffusion chambers which have been implanted subcutaneously into mice.
Adipocyte, Bone marrow, Chondrocyte, Diffusion chamber, Osteoblast, Stem cells
53-70
Kanczler, Janos
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Tare, Rahul S.
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Stumpf, Patrick
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Noble, Timothy J.
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Black, Cameron
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Oreffo, Richard O.C.
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Kanczler, Janos
eb8db9ff-a038-475f-9030-48eef2b0559c
Tare, Rahul S.
587c9db4-e409-4e7c-a02a-677547ab724a
Stumpf, Patrick
dfdb286c-b321-46d3-8ba2-85b3b4a7f092
Noble, Timothy J.
a40ab178-af61-421e-adef-b4270c4a2b85
Black, Cameron
80d8f092-7395-4e6f-967b-c828502904e3
Oreffo, Richard O.C.
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
Kanczler, Janos, Tare, Rahul S., Stumpf, Patrick, Noble, Timothy J., Black, Cameron and Oreffo, Richard O.C.
(2019)
Isolation, differentiation, and characterization of human bone marrow stem cells In vitro and in vivo.
In,
Idris, I.
(ed.)
Bone Research Protocols.
(Methods in Molecular Biology, 1914)
New York.
Humana, .
(doi:10.1007/978-1-4939-8997-3_4).
Record type:
Book Section
Abstract
In this chapter, we describe techniques for the isolation and characterisation of skeletal stem cells from human bone marrow. The methods for enrichment of STRO-1+ and STRO-4+ cells using magnetic activated cell sorting are described and we also detail techniques for establishing and characterizing osteogenic, adipogenic, and chondrogenic cultures from these cells. Finally, we present methods for studying the ability of these cells to produce bone in vivo using diffusion chambers which have been implanted subcutaneously into mice.
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e-pub ahead of print date: 8 February 2019
Keywords:
Adipocyte, Bone marrow, Chondrocyte, Diffusion chamber, Osteoblast, Stem cells
Identifiers
Local EPrints ID: 428370
URI: http://eprints.soton.ac.uk/id/eprint/428370
PURE UUID: 1c99d693-a4c3-414f-91f6-ad8d760513db
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Date deposited: 22 Feb 2019 17:30
Last modified: 16 Mar 2024 03:45
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Contributors
Author:
Janos Kanczler
Author:
Patrick Stumpf
Author:
Timothy J. Noble
Author:
Cameron Black
Editor:
I. Idris
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