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Lower limb ischemic preconditioning combined with dietary nitrate supplementation does not influence time-trial performance in well-trained cyclists

Lower limb ischemic preconditioning combined with dietary nitrate supplementation does not influence time-trial performance in well-trained cyclists
Lower limb ischemic preconditioning combined with dietary nitrate supplementation does not influence time-trial performance in well-trained cyclists

Objectives: Dietary nitrate (NO3 ) supplementation and ischaemic preconditioning (IPC) can independently improve exercise performance. The purpose of this study was to explore whether NO3 supplementation, ingested prior to an IPC protocol, could synergistically enhance parameters of exercise. Design: Double-blind randomized crossover trial. Methods: Ten competitive male cyclists (age 34 ± 6 years, body mass 78.9 ± 4.9 kg, V⋅O2peak 55 ± 4 mL kg min−1) completed an incremental exercise test followed by three cycling trials comprising a square-wave submaximal component and a 16.1 km time-trial. Oxygen uptake (V⋅O2) and muscle oxygenation kinetics were measured throughout. The baseline (BASE) trial was conducted without any dietary intervention or IPC. In the remaining two trials, participants received 3 × 5 min bouts of lower limb bilateral IPC prior to exercise. Participants ingested NO3 -rich gel (NIT + IPC) 90 min prior to testing in one trial and a low NO3 placebo in the other (PLA + IPC). Plasma NO3 and nitrite (NO2 ) were measured immediately before and after application of IPC. Results: Plasma [NO3 ] and [NO2 ] were higher before and after IPC in NIT + IPC compared to BASE (P < 0.001) but did not differ between BASE and PLA + IPC. There were no differences in V⋅O2 kinetics or muscle oxygenation parameters between trials (all P > 0.4). Performance in the time-trial was similar between trials (BASE 1343 ± 72 s, PLA + IPC 1350 ± 75 s, NIT + IPC 1346 ± 83 s, P = 0.98). Conclusions: Pre-exercise IPC did not improve sub-maximal exercise or performance measures, either alone or in combination with dietary NO3 supplementation.

Blood flow, Exercise, Hyperaemia, Nitric oxide, Nitrite
1440-2440
McIlvenna, Luke C.
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Muggeridge, David J.
336fd016-225f-485b-9122-dea4455ab7f3
Forrest (Nee Whyte), Laura J.
71ae5796-de4c-4a7d-951f-5e6534adda16
Monaghan, Chris
58f45991-cd8e-49a6-af16-158cc0e27a8d
Liddle, Luke
fc412297-ec88-4d99-a3c2-9570b5e682e9
Burleigh, Mia C.
edd37f86-6afd-4e16-86b2-720e062ff2ed
Sculthorpe, Nicholas
72e3518f-f09c-4457-a881-85715959b965
Fernandez, Bernadette O.
9890aabc-1fe6-4530-a51e-31182e537131
Feelisch, Martin
8c1b9965-8614-4e85-b2c6-458a2e17eafd
Easton, Chris
76f6cbdd-284c-477f-8d6a-5d11aaced94c
McIlvenna, Luke C.
14e111c7-3882-41e1-b104-7c2ebb6bbe85
Muggeridge, David J.
336fd016-225f-485b-9122-dea4455ab7f3
Forrest (Nee Whyte), Laura J.
71ae5796-de4c-4a7d-951f-5e6534adda16
Monaghan, Chris
58f45991-cd8e-49a6-af16-158cc0e27a8d
Liddle, Luke
fc412297-ec88-4d99-a3c2-9570b5e682e9
Burleigh, Mia C.
edd37f86-6afd-4e16-86b2-720e062ff2ed
Sculthorpe, Nicholas
72e3518f-f09c-4457-a881-85715959b965
Fernandez, Bernadette O.
9890aabc-1fe6-4530-a51e-31182e537131
Feelisch, Martin
8c1b9965-8614-4e85-b2c6-458a2e17eafd
Easton, Chris
76f6cbdd-284c-477f-8d6a-5d11aaced94c

McIlvenna, Luke C., Muggeridge, David J., Forrest (Nee Whyte), Laura J., Monaghan, Chris, Liddle, Luke, Burleigh, Mia C., Sculthorpe, Nicholas, Fernandez, Bernadette O., Feelisch, Martin and Easton, Chris (2019) Lower limb ischemic preconditioning combined with dietary nitrate supplementation does not influence time-trial performance in well-trained cyclists. Journal of Science and Medicine in Sport. (doi:10.1016/j.jsams.2019.01.011).

Record type: Article

Abstract

Objectives: Dietary nitrate (NO3 ) supplementation and ischaemic preconditioning (IPC) can independently improve exercise performance. The purpose of this study was to explore whether NO3 supplementation, ingested prior to an IPC protocol, could synergistically enhance parameters of exercise. Design: Double-blind randomized crossover trial. Methods: Ten competitive male cyclists (age 34 ± 6 years, body mass 78.9 ± 4.9 kg, V⋅O2peak 55 ± 4 mL kg min−1) completed an incremental exercise test followed by three cycling trials comprising a square-wave submaximal component and a 16.1 km time-trial. Oxygen uptake (V⋅O2) and muscle oxygenation kinetics were measured throughout. The baseline (BASE) trial was conducted without any dietary intervention or IPC. In the remaining two trials, participants received 3 × 5 min bouts of lower limb bilateral IPC prior to exercise. Participants ingested NO3 -rich gel (NIT + IPC) 90 min prior to testing in one trial and a low NO3 placebo in the other (PLA + IPC). Plasma NO3 and nitrite (NO2 ) were measured immediately before and after application of IPC. Results: Plasma [NO3 ] and [NO2 ] were higher before and after IPC in NIT + IPC compared to BASE (P < 0.001) but did not differ between BASE and PLA + IPC. There were no differences in V⋅O2 kinetics or muscle oxygenation parameters between trials (all P > 0.4). Performance in the time-trial was similar between trials (BASE 1343 ± 72 s, PLA + IPC 1350 ± 75 s, NIT + IPC 1346 ± 83 s, P = 0.98). Conclusions: Pre-exercise IPC did not improve sub-maximal exercise or performance measures, either alone or in combination with dietary NO3 supplementation.

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Accepted/In Press date: 18 January 2019
e-pub ahead of print date: 26 January 2019
Keywords: Blood flow, Exercise, Hyperaemia, Nitric oxide, Nitrite

Identifiers

Local EPrints ID: 428451
URI: http://eprints.soton.ac.uk/id/eprint/428451
ISSN: 1440-2440
PURE UUID: 5edb98c4-3790-46d7-835f-81cc6e71622f
ORCID for Bernadette O. Fernandez: ORCID iD orcid.org/0000-0001-6337-0381
ORCID for Martin Feelisch: ORCID iD orcid.org/0000-0003-2320-1158

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Date deposited: 27 Feb 2019 17:30
Last modified: 16 Mar 2024 07:36

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Contributors

Author: Luke C. McIlvenna
Author: David J. Muggeridge
Author: Laura J. Forrest (Nee Whyte)
Author: Chris Monaghan
Author: Luke Liddle
Author: Mia C. Burleigh
Author: Nicholas Sculthorpe
Author: Bernadette O. Fernandez ORCID iD
Author: Martin Feelisch ORCID iD
Author: Chris Easton

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