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Preparation and characterisation of fluorescent mesoporous surfaces: towards the study of extracellular fluxes

Preparation and characterisation of fluorescent mesoporous surfaces: towards the study of extracellular fluxes
Preparation and characterisation of fluorescent mesoporous surfaces: towards the study of extracellular fluxes
This thesis describes the efforts made toward developing new structured surfaces. These new structured and modified surfaces presented are of great potential interest in the detection of cellular flux. They also provide an interesting synthetic challenge due to the requirement for the synthesis of complex multi-functional molecules and their immobilisation on a surface. The project also presents a challenge in terms of multidisciplinarity, as many different techniques from different areas of science need to be understood and applied.

The structured surfaces were deposited using a method developed by Alain Walcarius: Electrochemically assisted self-assembly (EASA). Fluorescent dyes, chosen to respond to a typical cellular flux, were synthesised and covalently attached onto the deposited surface. This report presents the synthesis of nine dyes, which can respond to pH, K+ or Zn2+.

The nine dyes were attached on the structured surfaces, the surfaces were characterised (cyclic voltammetry, Raman spectroscopy, microscopy) and its properties determined (pKa, quantum yield, selectivity). Ultimately, fluorescent microscopy will give numerous different possibilities for biological analysis. The future work will be to focus on growing cells on the surfaces and validate our methodology by studying the fluorescent response of the surface towards extracellular fluxes.
University of Southampton
Lethuillier, Victor
add5360e-082a-4f0a-a30a-6e4a1c70b467
Lethuillier, Victor
add5360e-082a-4f0a-a30a-6e4a1c70b467
Brown, Richard
21ce697a-7c3a-480e-919f-429a3d8550f5

Lethuillier, Victor (2018) Preparation and characterisation of fluorescent mesoporous surfaces: towards the study of extracellular fluxes. University of Southampton, Doctoral Thesis, 183pp.

Record type: Thesis (Doctoral)

Abstract

This thesis describes the efforts made toward developing new structured surfaces. These new structured and modified surfaces presented are of great potential interest in the detection of cellular flux. They also provide an interesting synthetic challenge due to the requirement for the synthesis of complex multi-functional molecules and their immobilisation on a surface. The project also presents a challenge in terms of multidisciplinarity, as many different techniques from different areas of science need to be understood and applied.

The structured surfaces were deposited using a method developed by Alain Walcarius: Electrochemically assisted self-assembly (EASA). Fluorescent dyes, chosen to respond to a typical cellular flux, were synthesised and covalently attached onto the deposited surface. This report presents the synthesis of nine dyes, which can respond to pH, K+ or Zn2+.

The nine dyes were attached on the structured surfaces, the surfaces were characterised (cyclic voltammetry, Raman spectroscopy, microscopy) and its properties determined (pKa, quantum yield, selectivity). Ultimately, fluorescent microscopy will give numerous different possibilities for biological analysis. The future work will be to focus on growing cells on the surfaces and validate our methodology by studying the fluorescent response of the surface towards extracellular fluxes.

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Published date: September 2018

Identifiers

Local EPrints ID: 428651
URI: http://eprints.soton.ac.uk/id/eprint/428651
PURE UUID: 57b6b2ee-5e45-46af-b0af-5becc7b45218
ORCID for Richard Brown: ORCID iD orcid.org/0000-0003-0156-7087

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Date deposited: 05 Mar 2019 17:30
Last modified: 01 Mar 2020 05:01

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