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Modular pressure and flow rate-balanced microfluidic serial dilution networks for miniaturised point-of-care diagnostic platforms

Modular pressure and flow rate-balanced microfluidic serial dilution networks for miniaturised point-of-care diagnostic platforms
Modular pressure and flow rate-balanced microfluidic serial dilution networks for miniaturised point-of-care diagnostic platforms

Fast, efficient and more importantly accurate serial dilution is a necessary requirement for most biochemical microfluidic-based quantitative diagnostic applications. Over the last two decades, a multitude of microfluidic devices has been proposed, each one demonstrating either a different type of dilution technique or complex system architecture based on various flow source and valving combinations. In this work, a novel serial dilution network architecture is demonstrated, implemented on two entirely different substrates for validation and performance characterisation. The single layer, stepwise serial diluter comprises an optimised microfluidic network, where identical dilution ratios per stage are ensured, either by applying equal pressure or equal flow rates at both inlets. The advantages of this serial diluter are twofold: Firstly, it is structured as a modular unit cell, simplifying the required fluid driving mechanism to a single source for both sample and buffer solution. Thus, this unit cell can be used as a fundamental microfluidic building block, forming multistage serial dilution cascades, once combined appropriately with itself or other similar unit cells. Secondly, the serial diluter can tolerate the inevitable flow source fluctuations, ensuring constant dilution ratios without the need to employ damping mechanisms, making it ideal for Point of Care (PoC) platforms. Proof-of-concept experiments with glucose have demonstrated good agreement between simulations and measurements, highlighting the validity of our serial diluter.

flow rate balance, Lab-on-PCB, Microfluidics, modular serial diluter, PCB manufactured microfluidics, PMMA microfluidics, Point-of-Care diagnostics, pressure balance, serial diluter, step wise diluter
1424-8220
Vasilakis, Nikolaos
7b5d4280-8c8d-4e55-8d58-6a38ab5c0bb2
Papadimitriou, Konstantinos I.
c0535540-f862-41b1-9cf3-92b1f46a4145
Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174
Prodromakis, Themistoklis
d58c9c10-9d25-4d22-b155-06c8437acfbf
Vasilakis, Nikolaos
7b5d4280-8c8d-4e55-8d58-6a38ab5c0bb2
Papadimitriou, Konstantinos I.
c0535540-f862-41b1-9cf3-92b1f46a4145
Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174
Prodromakis, Themistoklis
d58c9c10-9d25-4d22-b155-06c8437acfbf

Vasilakis, Nikolaos, Papadimitriou, Konstantinos I., Morgan, Hywel and Prodromakis, Themistoklis (2019) Modular pressure and flow rate-balanced microfluidic serial dilution networks for miniaturised point-of-care diagnostic platforms. Sensors, 19 (4). (doi:10.3390/s19040911).

Record type: Article

Abstract

Fast, efficient and more importantly accurate serial dilution is a necessary requirement for most biochemical microfluidic-based quantitative diagnostic applications. Over the last two decades, a multitude of microfluidic devices has been proposed, each one demonstrating either a different type of dilution technique or complex system architecture based on various flow source and valving combinations. In this work, a novel serial dilution network architecture is demonstrated, implemented on two entirely different substrates for validation and performance characterisation. The single layer, stepwise serial diluter comprises an optimised microfluidic network, where identical dilution ratios per stage are ensured, either by applying equal pressure or equal flow rates at both inlets. The advantages of this serial diluter are twofold: Firstly, it is structured as a modular unit cell, simplifying the required fluid driving mechanism to a single source for both sample and buffer solution. Thus, this unit cell can be used as a fundamental microfluidic building block, forming multistage serial dilution cascades, once combined appropriately with itself or other similar unit cells. Secondly, the serial diluter can tolerate the inevitable flow source fluctuations, ensuring constant dilution ratios without the need to employ damping mechanisms, making it ideal for Point of Care (PoC) platforms. Proof-of-concept experiments with glucose have demonstrated good agreement between simulations and measurements, highlighting the validity of our serial diluter.

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More information

Accepted/In Press date: 12 February 2019
Published date: 21 February 2019
Keywords: flow rate balance, Lab-on-PCB, Microfluidics, modular serial diluter, PCB manufactured microfluidics, PMMA microfluidics, Point-of-Care diagnostics, pressure balance, serial diluter, step wise diluter

Identifiers

Local EPrints ID: 428703
URI: https://eprints.soton.ac.uk/id/eprint/428703
ISSN: 1424-8220
PURE UUID: 2c0eece6-c644-4466-b41a-8b15748fd6a1
ORCID for Hywel Morgan: ORCID iD orcid.org/0000-0003-4850-5676
ORCID for Themistoklis Prodromakis: ORCID iD orcid.org/0000-0002-6267-6909

Catalogue record

Date deposited: 07 Mar 2019 17:30
Last modified: 20 Jul 2019 00:59

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Contributors

Author: Nikolaos Vasilakis
Author: Konstantinos I. Papadimitriou
Author: Hywel Morgan ORCID iD

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