The University of Southampton
University of Southampton Institutional Repository

Dynamic 1H imaging of hyperpolarized [1-13C]lactate in vivo using a reverse INEPT experiment

Dynamic 1H imaging of hyperpolarized [1-13C]lactate in vivo using a reverse INEPT experiment
Dynamic 1H imaging of hyperpolarized [1-13C]lactate in vivo using a reverse INEPT experiment
Purpose:
Dynamic magnetic resonance spectroscopic imaging of hyperpolarized 13C-labeled cell substrates has enabled the investigation of tissue metabolism in vivo. Currently observation of these hyperpolarized substrates is limited mainly to 13C detection. We describe here an imaging pulse sequence that enables proton observation by using polarization transfer from the hyperpolarized 13C nucleus to spin-coupled protons.

Methods:
The pulse sequence transfers 13C hyperpolarization to 1H using a modified reverse insensitive nuclei enhanced by polarization transfer (INEPT) sequence that acquires a fully refocused echo. The resulting hyperpolarized 1H signal is acquired using a 2D echo-planar trajectory. The efficiency of polarization transfer was investigated using simulations with and without T1 and T2 relaxation of both the 1H and 13C nuclei.

Results:
Simulations showed that 1H detection of the hyperpolarized 13C nucleus in lactate should increase significantly the signal-to-noise ratio when compared with direct 13C detection at 3T. However the advantage of 1H detection is expected to disappear at higher fields. Dynamic 1H images of hyperpolarized [1-13C]lactate, with a spatial resolution of 1.25 × 1.25 mm2, were acquired from a phantom injected with hyperpolarized [1-13C]lactate and from tumors in vivo following injection of hyperpolarized [1-13C]pyruvate.

Conclusions:
The sequence allows 1H imaging of hyperpolarized 13C-labeled substrates in vivo. Magn Reson Med, 2017. © 2017 The Authors Magnetic Resonance in Medicine published by Wiley Periodicals, Inc. on behalf of International Society for Magnetic Resonance in Medicine. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
hyperpolization, Imaging, pyruvate, lactate
0740-3194
Wang, Jiazheng
7539a9d6-310e-4607-b0f4-ede31293553b
Kreis, Felix
e9687c5c-8a6d-4ea4-b407-f8c0ba284d8a
Wright, Alan J.
fa8b30ea-13ba-4c19-b5bc-f92d330fd2c5
Hesketh, Richard L.
a7163e72-5691-4e60-b0cc-35fc49c0915b
Levitt, Malcolm H.
bcc5a80a-e5c5-4e0e-9a9a-249d036747c3
Brindle, Kevin M.
316bceba-bd08-4979-89b3-28d35adf882a
Wang, Jiazheng
7539a9d6-310e-4607-b0f4-ede31293553b
Kreis, Felix
e9687c5c-8a6d-4ea4-b407-f8c0ba284d8a
Wright, Alan J.
fa8b30ea-13ba-4c19-b5bc-f92d330fd2c5
Hesketh, Richard L.
a7163e72-5691-4e60-b0cc-35fc49c0915b
Levitt, Malcolm H.
bcc5a80a-e5c5-4e0e-9a9a-249d036747c3
Brindle, Kevin M.
316bceba-bd08-4979-89b3-28d35adf882a

Wang, Jiazheng, Kreis, Felix, Wright, Alan J., Hesketh, Richard L., Levitt, Malcolm H. and Brindle, Kevin M. (2017) Dynamic 1H imaging of hyperpolarized [1-13C]lactate in vivo using a reverse INEPT experiment. Magnetic Resonance in Medicine. (doi:10.1002/mrm.26725).

Record type: Article

Abstract

Purpose:
Dynamic magnetic resonance spectroscopic imaging of hyperpolarized 13C-labeled cell substrates has enabled the investigation of tissue metabolism in vivo. Currently observation of these hyperpolarized substrates is limited mainly to 13C detection. We describe here an imaging pulse sequence that enables proton observation by using polarization transfer from the hyperpolarized 13C nucleus to spin-coupled protons.

Methods:
The pulse sequence transfers 13C hyperpolarization to 1H using a modified reverse insensitive nuclei enhanced by polarization transfer (INEPT) sequence that acquires a fully refocused echo. The resulting hyperpolarized 1H signal is acquired using a 2D echo-planar trajectory. The efficiency of polarization transfer was investigated using simulations with and without T1 and T2 relaxation of both the 1H and 13C nuclei.

Results:
Simulations showed that 1H detection of the hyperpolarized 13C nucleus in lactate should increase significantly the signal-to-noise ratio when compared with direct 13C detection at 3T. However the advantage of 1H detection is expected to disappear at higher fields. Dynamic 1H images of hyperpolarized [1-13C]lactate, with a spatial resolution of 1.25 × 1.25 mm2, were acquired from a phantom injected with hyperpolarized [1-13C]lactate and from tumors in vivo following injection of hyperpolarized [1-13C]pyruvate.

Conclusions:
The sequence allows 1H imaging of hyperpolarized 13C-labeled substrates in vivo. Magn Reson Med, 2017. © 2017 The Authors Magnetic Resonance in Medicine published by Wiley Periodicals, Inc. on behalf of International Society for Magnetic Resonance in Medicine. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

Text
Wang_et_al-2017-Magnetic_Resonance_in_Medicine - Version of Record
Available under License Creative Commons Attribution.
Download (312kB)

More information

Accepted/In Press date: 29 March 2017
e-pub ahead of print date: 5 May 2017
Keywords: hyperpolization, Imaging, pyruvate, lactate

Identifiers

Local EPrints ID: 428907
URI: http://eprints.soton.ac.uk/id/eprint/428907
ISSN: 0740-3194
PURE UUID: a0889039-bb43-43fe-9894-85781c9e9b2f
ORCID for Malcolm H. Levitt: ORCID iD orcid.org/0000-0001-9878-1180

Catalogue record

Date deposited: 13 Mar 2019 19:34
Last modified: 16 Mar 2024 03:19

Export record

Altmetrics

Contributors

Author: Jiazheng Wang
Author: Felix Kreis
Author: Alan J. Wright
Author: Richard L. Hesketh
Author: Kevin M. Brindle

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×