Lipid driven nanodomains in giant lipid vesicles are fluid and disordered
Lipid driven nanodomains in giant lipid vesicles are fluid and disordered
It is a fundamental question in cell biology and biophysics whether sphingomyelin (SM)- and cholesterol (Chol)- driven nanodomains exist in living cells and in model membranes. Biophysical studies on model membranes revealed SM and Chol driven micrometer-sized liquid-ordered domains. Although the existence of such microdomains has not been proven for the plasma membrane, such lipid mixtures have been often used as a model system for ‘rafts’. On the other hand, recent super resolution and single molecule results indicate that the plasma membrane might organize into nanocompartments.
However, due to the limited resolution of those techniques their unambiguous characterization is still missing. In this work, a novel combination of Förster resonance energy transfer and Monte Carlo simulations (MC-FRET) identifies directly 10nm large nanodomains in liquid-disordered model membranes composed of lipid mixtures containing SM and Chol. Combining MC-FRET with solid-state wide-line and high resolution magic angle spinning NMR as well as with fluorescence correlation spectroscopy we demonstrate that these nanodomains containing hundreds of lipid molecules are fluid and disordered. In terms of their size, fluidity, order and lifetime these nanodomains may represent a relevant model system for cellular membranes and are closely related to nanocompartments suggested to exist in cellular membranes.
membrane biophysics
Alena, Koukalova
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Amaro, Mariana
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Aydogan, Gokcan
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Grobner, Gerhard
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Williamson, Philip T.F.
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Mikhalyov, Ilya
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Hof, Martin
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Sachl, Radek
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Alena, Koukalova
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Amaro, Mariana
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Aydogan, Gokcan
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Grobner, Gerhard
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Williamson, Philip T.F.
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Mikhalyov, Ilya
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Hof, Martin
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Sachl, Radek
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Alena, Koukalova, Amaro, Mariana, Aydogan, Gokcan, Grobner, Gerhard, Williamson, Philip T.F., Mikhalyov, Ilya, Hof, Martin and Sachl, Radek
(2017)
Lipid driven nanodomains in giant lipid vesicles are fluid and disordered.
Scientific Reports, 7, [5460].
(doi:10.1038/s41598-017-05539-y).
Abstract
It is a fundamental question in cell biology and biophysics whether sphingomyelin (SM)- and cholesterol (Chol)- driven nanodomains exist in living cells and in model membranes. Biophysical studies on model membranes revealed SM and Chol driven micrometer-sized liquid-ordered domains. Although the existence of such microdomains has not been proven for the plasma membrane, such lipid mixtures have been often used as a model system for ‘rafts’. On the other hand, recent super resolution and single molecule results indicate that the plasma membrane might organize into nanocompartments.
However, due to the limited resolution of those techniques their unambiguous characterization is still missing. In this work, a novel combination of Förster resonance energy transfer and Monte Carlo simulations (MC-FRET) identifies directly 10nm large nanodomains in liquid-disordered model membranes composed of lipid mixtures containing SM and Chol. Combining MC-FRET with solid-state wide-line and high resolution magic angle spinning NMR as well as with fluorescence correlation spectroscopy we demonstrate that these nanodomains containing hundreds of lipid molecules are fluid and disordered. In terms of their size, fluidity, order and lifetime these nanodomains may represent a relevant model system for cellular membranes and are closely related to nanocompartments suggested to exist in cellular membranes.
Text
s41598-017-05539-y
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Submitted date: 31 January 2016
Accepted/In Press date: 30 May 2017
e-pub ahead of print date: 14 July 2017
Keywords:
membrane biophysics
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Local EPrints ID: 428909
URI: http://eprints.soton.ac.uk/id/eprint/428909
ISSN: 2045-2322
PURE UUID: 43d167ec-0786-4d01-8494-7688af124117
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Date deposited: 13 Mar 2019 19:34
Last modified: 16 Mar 2024 03:53
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Contributors
Author:
Koukalova Alena
Author:
Mariana Amaro
Author:
Gokcan Aydogan
Author:
Gerhard Grobner
Author:
Ilya Mikhalyov
Author:
Martin Hof
Author:
Radek Sachl
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