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Lipid driven nanodomains in giant lipid vesicles are fluid and disordered

Lipid driven nanodomains in giant lipid vesicles are fluid and disordered
Lipid driven nanodomains in giant lipid vesicles are fluid and disordered
It is a fundamental question in cell biology and biophysics whether sphingomyelin (SM)- and cholesterol (Chol)- driven nanodomains exist in living cells and in model membranes. Biophysical studies on model membranes revealed SM and Chol driven micrometer-sized liquid-ordered domains. Although the existence of such microdomains has not been proven for the plasma membrane, such lipid mixtures have been often used as a model system for ‘rafts’. On the other hand, recent super resolution and single molecule results indicate that the plasma membrane might organize into nanocompartments.
However, due to the limited resolution of those techniques their unambiguous characterization is still missing. In this work, a novel combination of Förster resonance energy transfer and Monte Carlo simulations (MC-FRET) identifies directly 10nm large nanodomains in liquid-disordered model membranes composed of lipid mixtures containing SM and Chol. Combining MC-FRET with solid-state wide-line and high resolution magic angle spinning NMR as well as with fluorescence correlation spectroscopy we demonstrate that these nanodomains containing hundreds of lipid molecules are fluid and disordered. In terms of their size, fluidity, order and lifetime these nanodomains may represent a relevant model system for cellular membranes and are closely related to nanocompartments suggested to exist in cellular membranes.
membrane biophysics
2045-2322
Alena, Koukalova
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Amaro, Mariana
81ceaf12-ad44-4864-83c0-dec7d009896a
Aydogan, Gokcan
828e7eb3-72af-471d-afc8-4c180e13acbf
Grobner, Gerhard
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Williamson, Philip T.F.
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Mikhalyov, Ilya
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Hof, Martin
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Sachl, Radek
abbf7e08-729a-47ce-aa98-ace8c23c61b7
Alena, Koukalova
7ed1f144-5ae6-4a78-8d97-77527781314b
Amaro, Mariana
81ceaf12-ad44-4864-83c0-dec7d009896a
Aydogan, Gokcan
828e7eb3-72af-471d-afc8-4c180e13acbf
Grobner, Gerhard
e6a72ea6-814e-4c48-b6b9-b20fe2763c7c
Williamson, Philip T.F.
0b7715c6-b60e-4e95-a1b1-6afc8b9f372a
Mikhalyov, Ilya
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Hof, Martin
fc58804b-9250-461f-9b73-fe4a1cbb410d
Sachl, Radek
abbf7e08-729a-47ce-aa98-ace8c23c61b7

Alena, Koukalova, Amaro, Mariana, Aydogan, Gokcan, Grobner, Gerhard, Williamson, Philip T.F., Mikhalyov, Ilya, Hof, Martin and Sachl, Radek (2017) Lipid driven nanodomains in giant lipid vesicles are fluid and disordered. Scientific Reports, 7. (doi:10.1038/s41598-017-05539-y).

Record type: Article

Abstract

It is a fundamental question in cell biology and biophysics whether sphingomyelin (SM)- and cholesterol (Chol)- driven nanodomains exist in living cells and in model membranes. Biophysical studies on model membranes revealed SM and Chol driven micrometer-sized liquid-ordered domains. Although the existence of such microdomains has not been proven for the plasma membrane, such lipid mixtures have been often used as a model system for ‘rafts’. On the other hand, recent super resolution and single molecule results indicate that the plasma membrane might organize into nanocompartments.
However, due to the limited resolution of those techniques their unambiguous characterization is still missing. In this work, a novel combination of Förster resonance energy transfer and Monte Carlo simulations (MC-FRET) identifies directly 10nm large nanodomains in liquid-disordered model membranes composed of lipid mixtures containing SM and Chol. Combining MC-FRET with solid-state wide-line and high resolution magic angle spinning NMR as well as with fluorescence correlation spectroscopy we demonstrate that these nanodomains containing hundreds of lipid molecules are fluid and disordered. In terms of their size, fluidity, order and lifetime these nanodomains may represent a relevant model system for cellular membranes and are closely related to nanocompartments suggested to exist in cellular membranes.

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s41598-017-05539-y - Version of Record
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Submitted date: 31 January 2016
Accepted/In Press date: 30 May 2017
e-pub ahead of print date: 14 July 2017
Keywords: membrane biophysics

Identifiers

Local EPrints ID: 428909
URI: https://eprints.soton.ac.uk/id/eprint/428909
ISSN: 2045-2322
PURE UUID: 43d167ec-0786-4d01-8494-7688af124117
ORCID for Philip T.F. Williamson: ORCID iD orcid.org/0000-0002-0231-8640

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Date deposited: 13 Mar 2019 19:34
Last modified: 15 Aug 2019 00:42

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Contributors

Author: Koukalova Alena
Author: Mariana Amaro
Author: Gokcan Aydogan
Author: Gerhard Grobner
Author: Ilya Mikhalyov
Author: Martin Hof
Author: Radek Sachl

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