READ ME File For 'Thesis data - The ecology, impact and management feasibility of the invasive kelp Undaria pinnatifida in the UK' Dataset DOI: 10.5258/SOTON/D0840 ReadMe Author: Graham Epstein, University of Southampton This dataset supports the publication: Epstein, G The ecology, impact and management feasibility of the invasive kelp Undaria pinnatifida in the UK PhD Thesis Contents +++++++++ This dataset contains data which are used for generating all main figures and tables within the thesis. The applicable data sheet for each figure and table are shown below. Code for the construction of each table and figure are not supplied as they require multiple code files, data manipulations, packages and software types (including some of which are not code driven). List of Figures 2.1 Southwest UK survey locations . . . . . . . . . . . . . . . . . . . . . . . . 37 Chpt 2 - Snorkel Transects 2.2 Example coastal survey site . . . . . . . . . . . . . . . . . . . . . . . . . . 40 Chpt 2 - Snorkel Transects 2.3 Undaria SACFOR at marina and coastal survey sites . . . . . . . . . . . . 44 Chpt 2 - Snorkel Transects Chpt 2- Marinas 2.4 Attributes of marinas within survey locations . . . . . . . . . . . . . . . . 45 Chpt 2 - Site Level Factors 2.5 Exposure and canopy community at coastal survey sites . . . . . . . . . . 47 Chpt 2 - Site Level Factors Chpt 2 - Snorkel Transects 2.6 Relationship between Undaria abundance and SACFOR . . . . . . . . . . 48 Chpt 2 - Snorkel Transects 2.7 Binomial models for Undaria presence-absence . . . . . . . . . . . . . . . 52 Chpt 2 - Snorkel Transects 2.8 Neagtive binomial models for Undaria abundance . . . . . . . . . . . . . . 53 Chpt 2 - Snorkel Transects 2.9 Neagtive binomial models for Undaria SACFOR . . . . . . . . . . . . . . 54 Chpt 2 - Snorkel Transects 3.1 Study sites for population dynamics study . . . . . . . . . . . . . . . . . . 67 NA 3.2 Developmental stages of Undaria sporophytes . . . . . . . . . . . . . . . . 69 NA 3.3 Morphological attributes measured for Undaria sporophytes . . . . . . . . 70 Chpt 3 - Sporophyte Data 3.4 An example of flow cytometry density plots . . . . . . . . . . . . . . . . . 72 NA 3.5 Mean abundance and biomass of Undaria in each habitat . . . . . . . . . 74 Chpts 3 & 5 - Quadrat Data 3.6 Mean proportion of recruits, mature and senescing sporophytes of Undaria in each habitat . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 76 Chpts 3 & 5 - Quadrat Data 3.7 Proportion of each developmental stage of Undaria in each habitat . . . . 77 Chpts 3 & 5 - Quadrat Data 3.8 Mean reproductive activity of Undaria in each habitat . . . . . . . . . . . 78 Chpt 3 - Sporophyte Data 3.9 tmMDS plots of bootstrapped average morphological data within each habitat . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80 Chpt 3 - Sporophyte Data 4.1 Study sites for marina removal study . . . . . . . . . . . . . . . . . . . . . 91 NA 4.2 Recruits of the four kelp species that can be found within marinas in Plymouth . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 93 NA 4.3 Mean number of Undaria recruits found in four removal treatments . . . . 96 Chpt 4 - Recruitment Data 4.4 Regression splines indicating the e ect of duration and month on removal treatments . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 98 Chpt 4 - Recruitment Data 4.5 Comparison of Undaria recruitment between unmanipulated areas and removal treatments . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 99 Chpt 4 - Recruitment Data Chpt 4 - Background Recruitment 5.1 Study sites for coexistence study . . . . . . . . . . . . . . . . . . . . . . . 111 NA 5.2 Mean density and biomass of canopy forming macroalgae in each habitat, averaging across three years . . . . . . . . . . . . . . . . . . . . . . . . . . 116 Chpts 3 & 5 - Quadrat Data 5.3 tmMDS plots of bootstrapped average monitoring data within each year of study . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 116 Chpts 3 & 5 - Quadrat Data 5.4 Spatial relationship between Undaria and co-occurring canopy formers . . 118 Chpts 3 & 5 - Quadrat Data 5.5 Distribution of canopy-forming macroalgae across depth . . . . . . . . . . 119 Chpt 5 - Depth Surveys 5.6 Change in density and percent cover of canopy-forming macroalgae following introduction of new substrate into a marina habitat . . . . . . . . 120 Chpt 5 - Marina Sucession Abund Chpt 5 Marina Sucession Perc 5.7 Change in density and percent cover of canopy-forming macroalgae following introduction of cleared substrate into intertidal reef habitat . . . . 121 Chpt 5 - Reef Sucession Abund Chpt 5 Reff Sucession Perc 6.1 Study site for press-removal study and examples of treatment plots . . . . 132 NA 6.2 Undaria removed and remaining during press-removal, and abundance and cover from monitoring . . . . . . . . . . . . . . . . . . . . . . . . . . . 138 NA 6.3 Temporal monitoring of macroalgae in press-removal treatments . . . . . 139 Chpt 6 - Temporal Abundance Chpt 6 - Temporal Percent Chpt 6 - Temporal Understory 6.4 End point sampling of native canopy-forming macroalgae in press-removal treatments . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 140 Chpt 6 - Final Abundance Chpt 6 - Final Biomass 6.5 End point sampling of native understory macroalgae within press-removal treatments . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 141 Chpt 6 - Final Understory 6.6 Principal component analysis of multivariate biochemical response metrics 143 Chpt 6 - FTIR Chpt 6 - CHN 6.7 Boxplots indicating the relative values of biochemical response metrics . . 144 Chpt 6 - FTIR Chpt 6 - CHN List of Tables 2.1 Range of site characteristics across each survey location . . . . . . . . . . 46 NA 2.2 ZINB model backwards selection . . . . . . . . . . . . . . . . . . . . . . . 49 Chpt 2 - Snorkel Transects 2.3 Optimal ZINB models . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50 Chpt 2 - Snorkel Transects 3.1 Description of Undaria developmental stages . . . . . . . . . . . . . . . . 68 NA 3.2 Results from the optimal ANOVA models, testing for difference in the abundance and biomass of Undaria . . . . . . . . . . . . . . . . . . . . . . 75 Chpts 3 & 5 - Quadrat Data 3.3 Results from the optimal PERMANOVA model, testing for difference in population structure of Undaria . . . . . . . . . . . . . . . . . . . . . . . 76 Chpts 3 & 5 - Quadrat Data 3.4 Results from the optimal ANOVA models, testing for difference in two reproductive activity metrics of Undaria . . . . . . . . . . . . . . . . . . . 78 Chpt 3 - Sporophyte Data 4.1 Summary of GAMMs predicting recruits within each removal treatment . 97 Chpt 4 - Recruitment Data 4.2 Post-hoc comparison of pairwise intercept diferences between removal factors from GAMMss . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 98 Chpt 4 - Recruitment Data 4.3 LR tests for comparing each removal treatment to unmanipulated recruitment data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 100 Chpt 4 - Recruitment Data Chpt 4 - Background Recruitment 5.1 GLMMS and LMMs identifying the in uence of co-occurring canopyforming macroalgae on the density and biomass of Undaria within each habitat. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 117 Chpts 3 & 5 - Quadrat Data 6.1 Multivariate GLMs testing for the effect of treatment and sampling month on native canopy-forming and understory macroalgae . . . . . . . . . . . . 138 Chpt 6 - Temporal Abundance Chpt 6 - Temporal Percent Chpt 6 - Temporal Understory Chpt 6 - Final Abundance Chpt 6 - Final Biomass Chpt 6 - Final Understory 6.2 Univariate post-hoc test statistics from multivariate GLMs testing for the effect of treatment on native canopy-forming macroalgae . . . . . . . . . . 141 Chpt 6 - Temporal Abundance Chpt 6 - Temporal Percent Chpt 6 - Final Abundance Chpt 6 - Final Biomass 6.3 Pairwise tests between treatment levels for canopy-forming macroalgae species with signiffcant univariate treatment effects . . . . . . . . . . . . . 142 Chpt 6 - Temporal Abundance Chpt 6 - Temporal Percent Chpt 6 - Final Abundance Chpt 6 - Final Biomass 6.4 Univariate post-hoc test statistics from multivariate LMs testing for the effect of treatment on biochemistry of native kelps . . . . . . . . . . . . . 143 Chpt 6 - FTIR Chpt 6 - CHN 6.5 Pairwise tests between treatment levels for kelps with signi cant univariate biochemical treatment e ects . . . . . . . . . . . . . . . . . . . . . . . 144 Chpt 6 - FTIR Chpt 6 - CHN Date of data collection: March 2016 - September 2018 Dataset available under a CC BY 4.0 licence Publisher: University of Southampton, U.K. Date: March, 2019