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A marine biofilm flow cell for in situ determination of drag and biofilm structure

A marine biofilm flow cell for in situ determination of drag and biofilm structure
A marine biofilm flow cell for in situ determination of drag and biofilm structure
It is not straightforward to link biofilm parameters to frictional drag, because of the heterogeneous distribution and viscoelasticity of the produced matrix. Here we present the design and calibration of a flow cell in which marine biofilms can be cultured under flow and then assessed for drag, structural and mechanical properties. The flow cell test section comprised a rectangular channel constructed by sandwiching together rigid PVC panels and side panels of clear acrylic, which allow natural light to enter. The Fanning friction factor (Cf) of the flow cell was found by measuring the pressure drop (ΔP) at various flow velocities (u). Flow cell calibration was carried out using a clean inert marine coating, and various roughness grades of sandpaper sheets to find Cf for each rigid roughness. ΔP was proportional to u2, indicating flow was turbulent (R2 = 0.99). The top panel of the flow cell can be substituted with a clear acrylic lid to allow simultaneous measurement of Cf and biofilm physico-mechanical properties by optical coherence tomography (OCT). Here, we demonstrated that the flow cell can be used to image microbial fouling using OCT. Future experiments will assess physico-mechanical and drag properties of marine fouling biofilms under flow.
biofilms, marine biofilms, flow cell, fouling, drag, OCT
0029-8018
59-65
Fabbri, Stefania
c93b6166-2117-48a9-9a88-b23a62c7b5da
Dennington, Simon
6a329a55-8c10-4515-8920-d8f40f302221
Price, Clayton
400e5283-6c21-4335-9cfd-8a008f5adfef
Longyear, Jennifer
fbc8ed1c-9fe4-45c3-bda4-7ca64551e5ce
Stoodley, Paul
08614665-92a9-4466-806e-20c6daeb483f
Fabbri, Stefania
c93b6166-2117-48a9-9a88-b23a62c7b5da
Dennington, Simon
6a329a55-8c10-4515-8920-d8f40f302221
Price, Clayton
400e5283-6c21-4335-9cfd-8a008f5adfef
Longyear, Jennifer
fbc8ed1c-9fe4-45c3-bda4-7ca64551e5ce
Stoodley, Paul
08614665-92a9-4466-806e-20c6daeb483f

Fabbri, Stefania, Dennington, Simon, Price, Clayton, Longyear, Jennifer and Stoodley, Paul (2019) A marine biofilm flow cell for in situ determination of drag and biofilm structure. Ocean Engineering, 178, 59-65. (doi:10.1016/j.oceaneng.2019.02.066).

Record type: Article

Abstract

It is not straightforward to link biofilm parameters to frictional drag, because of the heterogeneous distribution and viscoelasticity of the produced matrix. Here we present the design and calibration of a flow cell in which marine biofilms can be cultured under flow and then assessed for drag, structural and mechanical properties. The flow cell test section comprised a rectangular channel constructed by sandwiching together rigid PVC panels and side panels of clear acrylic, which allow natural light to enter. The Fanning friction factor (Cf) of the flow cell was found by measuring the pressure drop (ΔP) at various flow velocities (u). Flow cell calibration was carried out using a clean inert marine coating, and various roughness grades of sandpaper sheets to find Cf for each rigid roughness. ΔP was proportional to u2, indicating flow was turbulent (R2 = 0.99). The top panel of the flow cell can be substituted with a clear acrylic lid to allow simultaneous measurement of Cf and biofilm physico-mechanical properties by optical coherence tomography (OCT). Here, we demonstrated that the flow cell can be used to image microbial fouling using OCT. Future experiments will assess physico-mechanical and drag properties of marine fouling biofilms under flow.

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FlowCellDesign_manuscript - Accepted Manuscript
Restricted to Repository staff only until 9 March 2020.
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More information

Accepted/In Press date: 24 February 2019
e-pub ahead of print date: 9 March 2019
Published date: 15 April 2019
Keywords: biofilms, marine biofilms, flow cell, fouling, drag, OCT

Identifiers

Local EPrints ID: 429332
URI: https://eprints.soton.ac.uk/id/eprint/429332
ISSN: 0029-8018
PURE UUID: 0444a60f-7576-4018-b94f-14b37ee04bfd
ORCID for Paul Stoodley: ORCID iD orcid.org/0000-0001-6069-273X

Catalogue record

Date deposited: 26 Mar 2019 17:30
Last modified: 20 Jul 2019 00:48

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Contributors

Author: Stefania Fabbri
Author: Clayton Price
Author: Jennifer Longyear
Author: Paul Stoodley ORCID iD

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