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Reactive species interactome alterations in oocyte donation pregnancies in the absence and presence of pre-eclampsia

Reactive species interactome alterations in oocyte donation pregnancies in the absence and presence of pre-eclampsia
Reactive species interactome alterations in oocyte donation pregnancies in the absence and presence of pre-eclampsia

In pregnancy, maternal physiology is subject to considerable adaptations, including alterations in cardiovascular and metabolic function as well as development of immunological tolerance towards the fetus. In an oocyte donation pregnancy, the fetus is fully allogeneic towards the mother, since it carries both oocyte donor antigens and paternal antigens. Therefore, oocyte donation pregnancies result in an immunologically challenging pregnancy, which is reflected by a higher-than-normal risk to develop pre-eclampsia. Based on the allogeneic conditions in oocyte donation pregnancies, we hypothesized that this situation may translate into alterations in concentration of stable readouts of constituents of the reactive species interactome (RSI) compared to normal pregnancies, especially serum free thiols, nitric oxide (NO) and hydrogen sulfide (H₂S) related metabolites. Indeed, total free thiol levels and nitrite (NO₂-) concentrations were significantly lower whereas protein-bound NO and sulfate (SO₄2-) concentrations were significantly higher in both oocyte donation and naturally conceived pregnancies complicated by pre-eclampsia. The increased concentrations of nitrite observed in uncomplicated oocyte donation pregnancies suggest that endothelial NO production is compensatorily enhanced to lower vascular tone. More research is warranted on the role of the RSI and bioenergetic status in uncomplicated oocyte donation pregnancies and oocyte donation pregnancies complicated by pre-eclampsia.

hydrogen sulfide, nitric oxide, oocyte donation, pre-eclampsia, reactive species interactome, thiols
1422-0067
1150
Bos, Manon
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Schoots, Mirthe H.
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Fernandez, Bernadette O.
9890aabc-1fe6-4530-a51e-31182e537131
Mikus-Lelinska, Monika
6ca48669-ff8f-4ae8-9d10-98c74327b4ec
Lau, Laurie C.
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Eikmans, Michael
0692b2da-639b-41ff-8750-37ae0ee36311
Goor, Harry van
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Gordijn, Sanne J.
af4d6a05-b182-4e9e-8743-ff695805cef2
Pasch, Andreas
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Feelisch, Martin
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Hoorn, Marie Louise P.van der
6abf2259-4ab0-42f6-ac97-c3f856e21e47
Bos, Manon
4b593bfb-93c2-4faa-a889-ef84cb4fdd72
Schoots, Mirthe H.
14e73e35-0e48-4da3-aeea-525577da1a3e
Fernandez, Bernadette O.
9890aabc-1fe6-4530-a51e-31182e537131
Mikus-Lelinska, Monika
6ca48669-ff8f-4ae8-9d10-98c74327b4ec
Lau, Laurie C.
2af8045d-6162-4939-aba7-28dd2f60f6a8
Eikmans, Michael
0692b2da-639b-41ff-8750-37ae0ee36311
Goor, Harry van
6e4f96a5-c749-43b6-a488-6af71f932dc3
Gordijn, Sanne J.
af4d6a05-b182-4e9e-8743-ff695805cef2
Pasch, Andreas
5f5d9b34-4d9e-40a4-9326-fa1cd352717d
Feelisch, Martin
8c1b9965-8614-4e85-b2c6-458a2e17eafd
Hoorn, Marie Louise P.van der
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Bos, Manon, Schoots, Mirthe H., Fernandez, Bernadette O., Mikus-Lelinska, Monika, Lau, Laurie C., Eikmans, Michael, Goor, Harry van, Gordijn, Sanne J., Pasch, Andreas, Feelisch, Martin and Hoorn, Marie Louise P.van der (2019) Reactive species interactome alterations in oocyte donation pregnancies in the absence and presence of pre-eclampsia. International Journal of Molecular Sciences, 20 (5), 1150. (doi:10.3390/ijms20051150).

Record type: Article

Abstract

In pregnancy, maternal physiology is subject to considerable adaptations, including alterations in cardiovascular and metabolic function as well as development of immunological tolerance towards the fetus. In an oocyte donation pregnancy, the fetus is fully allogeneic towards the mother, since it carries both oocyte donor antigens and paternal antigens. Therefore, oocyte donation pregnancies result in an immunologically challenging pregnancy, which is reflected by a higher-than-normal risk to develop pre-eclampsia. Based on the allogeneic conditions in oocyte donation pregnancies, we hypothesized that this situation may translate into alterations in concentration of stable readouts of constituents of the reactive species interactome (RSI) compared to normal pregnancies, especially serum free thiols, nitric oxide (NO) and hydrogen sulfide (H₂S) related metabolites. Indeed, total free thiol levels and nitrite (NO₂-) concentrations were significantly lower whereas protein-bound NO and sulfate (SO₄2-) concentrations were significantly higher in both oocyte donation and naturally conceived pregnancies complicated by pre-eclampsia. The increased concentrations of nitrite observed in uncomplicated oocyte donation pregnancies suggest that endothelial NO production is compensatorily enhanced to lower vascular tone. More research is warranted on the role of the RSI and bioenergetic status in uncomplicated oocyte donation pregnancies and oocyte donation pregnancies complicated by pre-eclampsia.

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Accepted/In Press date: 26 February 2019
Published date: 6 March 2019
Keywords: hydrogen sulfide, nitric oxide, oocyte donation, pre-eclampsia, reactive species interactome, thiols

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Local EPrints ID: 429602
URI: https://eprints.soton.ac.uk/id/eprint/429602
ISSN: 1422-0067
PURE UUID: c188167d-bca2-4d27-9972-496a9ae04c6d
ORCID for Bernadette O. Fernandez: ORCID iD orcid.org/0000-0001-6337-0381
ORCID for Martin Feelisch: ORCID iD orcid.org/0000-0003-2320-1158

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Date deposited: 01 Apr 2019 16:30
Last modified: 18 Jul 2019 10:17

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Contributors

Author: Manon Bos
Author: Mirthe H. Schoots
Author: Bernadette O. Fernandez ORCID iD
Author: Monika Mikus-Lelinska
Author: Laurie C. Lau
Author: Michael Eikmans
Author: Harry van Goor
Author: Sanne J. Gordijn
Author: Andreas Pasch
Author: Martin Feelisch ORCID iD
Author: Marie Louise P.van der Hoorn

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