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Evaluation of microscopic techniques to observe iron precipitation in a natural microbial biofilm

Evaluation of microscopic techniques to observe iron precipitation in a natural microbial biofilm
Evaluation of microscopic techniques to observe iron precipitation in a natural microbial biofilm
Iron biomineralization in a microbial biofilm consortium from Canadian Shield groundwaters has been investigated with different microscopic techniques. The advantages and disadvantages of the different methods of observing a biofilm growing on an opaque mineral surface are discussed. Scanning electron microscopy was able to show the initial attachment and dispersion of bacteria on the mineral surfaces, whereas transmission electron microscopy gave greater detail and revealed the precise location of the iron precipitation on cell surfaces, including S-layers, and also throughout the extrapolymeric slime of the biofilm. Episcopic Nomarski differential interference contrast microscopy allowed direct observation of biofilm dynamics and confirmed the precipitation of iron directly onto certain bacteria, which were then specifically ingested by protozoa. This novel ingestion of iron-coated bacteria by protozoans essentially eliminated iron from solution and trapped it within the biofilm. Over time in the natural environment, this iron, enmeshed within a biofilm, may become incorporated into iron-rich sediments.
Biofilm, Differential interference contrast microscopy, Iron biomineralization, Microscopic technique, Protozoa
0168-6496
297-310
Brown, D. Ann
59f03551-61b6-4bc2-b417-e96a6c4fba63
Beveridge, Terry J.
fc13b6f7-48c9-45a9-aca1-1f6cff400845
Keevil, C. William
cb7de0a7-ce33-4cfa-af52-07f99e5650eb
Sherriff, Barbara L.
ae9940b4-0f80-407e-93bd-628a58d9847f
Brown, D. Ann
59f03551-61b6-4bc2-b417-e96a6c4fba63
Beveridge, Terry J.
fc13b6f7-48c9-45a9-aca1-1f6cff400845
Keevil, C. William
cb7de0a7-ce33-4cfa-af52-07f99e5650eb
Sherriff, Barbara L.
ae9940b4-0f80-407e-93bd-628a58d9847f

Brown, D. Ann, Beveridge, Terry J., Keevil, C. William and Sherriff, Barbara L. (1998) Evaluation of microscopic techniques to observe iron precipitation in a natural microbial biofilm. FEMS Microbiology Ecology, 26 (4), 297-310. (doi:10.1016/S0168-6496(98)00044-0).

Record type: Article

Abstract

Iron biomineralization in a microbial biofilm consortium from Canadian Shield groundwaters has been investigated with different microscopic techniques. The advantages and disadvantages of the different methods of observing a biofilm growing on an opaque mineral surface are discussed. Scanning electron microscopy was able to show the initial attachment and dispersion of bacteria on the mineral surfaces, whereas transmission electron microscopy gave greater detail and revealed the precise location of the iron precipitation on cell surfaces, including S-layers, and also throughout the extrapolymeric slime of the biofilm. Episcopic Nomarski differential interference contrast microscopy allowed direct observation of biofilm dynamics and confirmed the precipitation of iron directly onto certain bacteria, which were then specifically ingested by protozoa. This novel ingestion of iron-coated bacteria by protozoans essentially eliminated iron from solution and trapped it within the biofilm. Over time in the natural environment, this iron, enmeshed within a biofilm, may become incorporated into iron-rich sediments.

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More information

Accepted/In Press date: 9 May 1998
Published date: 1 August 1998
Keywords: Biofilm, Differential interference contrast microscopy, Iron biomineralization, Microscopic technique, Protozoa

Identifiers

Local EPrints ID: 429840
URI: http://eprints.soton.ac.uk/id/eprint/429840
ISSN: 0168-6496
PURE UUID: 17eaa08f-5aba-4dd3-9068-4b0794e7cfd2
ORCID for C. William Keevil: ORCID iD orcid.org/0000-0003-1917-7706

Catalogue record

Date deposited: 08 Apr 2019 16:30
Last modified: 18 Feb 2021 16:58

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