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Influence of oxygen availability on physiology, verocytotoxin expression and adherence of Escherichia coli 0157

Influence of oxygen availability on physiology, verocytotoxin expression and adherence of Escherichia coli 0157
Influence of oxygen availability on physiology, verocytotoxin expression and adherence of Escherichia coli 0157
A strain of Escherichia coli serotype 0157 was grown in steady state chemostat culture under aerobic, oxygen-limited and anaerobic conditions. The growth and metabolic efficiency of oxygen-limited and anaerobic cultures was impaired, with biomass yield and the molar growth yield for glucose, Y(glucose), reduced markedly in comparison with aerobic cultures. Steady state cells were typically short rods 2-3 μm long, and were encapsulated by a layer of extracellular material. The majority of cells were non-flagellated and fimbriae were not observed. Chemostat-grown cells were significantly more adhesive for HEp-2 monolayers than cells grown in aerobic batch culture. Furthermore, oxygen-limited and anaerobic cultures were significantly more adhesive for Hep-2 cells when compared with cells grown in aerobic chemostat culture, possibly reflecting increased pathogenicity associated with the induction of novel adhesins. Type 1 pill were not responsible for increased adherence. Verocytotoxins, VT1 and VT2, were expressed constitutively and were not influenced by oxygen availability. This study demonstrates that E. coli 0157 is a versatile micro-organism, which responds to environmental conditions likely to be encountered during infection by inducing a phenotype which is more adhesive for human epithelial cells.
1364-5072
117-124
James, Brian W.
2982ef34-33f6-4dd1-bc54-03b0333f3339
Keevil, C. W.
cb7de0a7-ce33-4cfa-af52-07f99e5650eb
James, Brian W.
2982ef34-33f6-4dd1-bc54-03b0333f3339
Keevil, C. W.
cb7de0a7-ce33-4cfa-af52-07f99e5650eb

James, Brian W. and Keevil, C. W. (1999) Influence of oxygen availability on physiology, verocytotoxin expression and adherence of Escherichia coli 0157. Journal of Applied Microbiology, 86 (1), 117-124. (doi:10.1046/j.1365-2672.1999.00639.x).

Record type: Article

Abstract

A strain of Escherichia coli serotype 0157 was grown in steady state chemostat culture under aerobic, oxygen-limited and anaerobic conditions. The growth and metabolic efficiency of oxygen-limited and anaerobic cultures was impaired, with biomass yield and the molar growth yield for glucose, Y(glucose), reduced markedly in comparison with aerobic cultures. Steady state cells were typically short rods 2-3 μm long, and were encapsulated by a layer of extracellular material. The majority of cells were non-flagellated and fimbriae were not observed. Chemostat-grown cells were significantly more adhesive for HEp-2 monolayers than cells grown in aerobic batch culture. Furthermore, oxygen-limited and anaerobic cultures were significantly more adhesive for Hep-2 cells when compared with cells grown in aerobic chemostat culture, possibly reflecting increased pathogenicity associated with the induction of novel adhesins. Type 1 pill were not responsible for increased adherence. Verocytotoxins, VT1 and VT2, were expressed constitutively and were not influenced by oxygen availability. This study demonstrates that E. coli 0157 is a versatile micro-organism, which responds to environmental conditions likely to be encountered during infection by inducing a phenotype which is more adhesive for human epithelial cells.

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More information

Accepted/In Press date: 10 August 1998
Published date: January 1999

Identifiers

Local EPrints ID: 429841
URI: http://eprints.soton.ac.uk/id/eprint/429841
ISSN: 1364-5072
PURE UUID: 443eab33-31bd-4382-9521-eee4d1306c45
ORCID for C. W. Keevil: ORCID iD orcid.org/0000-0003-1917-7706

Catalogue record

Date deposited: 08 Apr 2019 16:30
Last modified: 07 Oct 2020 08:32

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