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Development and application of a novel peptide nucleic acid probe for the specific detection of Helicobacter pylori in gastric biopsy specimens

Development and application of a novel peptide nucleic acid probe for the specific detection of Helicobacter pylori in gastric biopsy specimens
Development and application of a novel peptide nucleic acid probe for the specific detection of Helicobacter pylori in gastric biopsy specimens
In this work, a fluorescence in situ hybridization (FISH) method for the rapid detection of Helicobacter pylori using a novel peptide nucleic acid (PNA) probe is reported. Laboratory testing with several different bacterial species, including other Helicobacter spp., has shown that this probe is highly specific for H. pylori strains. In addition, the PNA FISH method has been successfully adapted for detection of the pathogen in paraffin-embedded gastric biopsy specimens. Copyright © 2007, American Society for Microbiology. All Rights Reserved.
0095-1137
3089-3094
Guimarães, N.
19b71f30-1a78-4a07-9aad-5e2abc1115bb
Azevedo, N. F.
79da2c36-313d-41a4-84a1-caaa3f6b0264
Figueiredo, C.
ef79ccf3-34dd-4695-b087-7ece834695b2
Keevil, C. W.
cb7de0a7-ce33-4cfa-af52-07f99e5650eb
Vieira, M. J.
45deadf9-bf20-4e60-95e2-506226f0fed2
Guimarães, N.
19b71f30-1a78-4a07-9aad-5e2abc1115bb
Azevedo, N. F.
79da2c36-313d-41a4-84a1-caaa3f6b0264
Figueiredo, C.
ef79ccf3-34dd-4695-b087-7ece834695b2
Keevil, C. W.
cb7de0a7-ce33-4cfa-af52-07f99e5650eb
Vieira, M. J.
45deadf9-bf20-4e60-95e2-506226f0fed2

Guimarães, N., Azevedo, N. F., Figueiredo, C., Keevil, C. W. and Vieira, M. J. (2007) Development and application of a novel peptide nucleic acid probe for the specific detection of Helicobacter pylori in gastric biopsy specimens. Journal of Clinical Microbiology, 45 (9), 3089-3094. (doi:10.1128/JCM.00858-07).

Record type: Article

Abstract

In this work, a fluorescence in situ hybridization (FISH) method for the rapid detection of Helicobacter pylori using a novel peptide nucleic acid (PNA) probe is reported. Laboratory testing with several different bacterial species, including other Helicobacter spp., has shown that this probe is highly specific for H. pylori strains. In addition, the PNA FISH method has been successfully adapted for detection of the pathogen in paraffin-embedded gastric biopsy specimens. Copyright © 2007, American Society for Microbiology. All Rights Reserved.

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Accepted/In Press date: 31 May 2007
Published date: September 2007
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Identifiers

Local EPrints ID: 429848
URI: https://eprints.soton.ac.uk/id/eprint/429848
DOI: doi:10.1128/JCM.00858-07
ISSN: 0095-1137
PURE UUID: 85bb3d18-c8a6-4fcd-b24e-da80b55c92a8
ORCID for C. W. Keevil: ORCID iD orcid.org/0000-0003-1917-7706

Catalogue record

Date deposited: 08 Apr 2019 16:30
Last modified: 26 Nov 2019 01:51

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Contributors

Author: N. Guimarães
Author: N. F. Azevedo
Author: C. Figueiredo
Author: C. W. Keevil ORCID iD
Author: M. J. Vieira

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