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New Adventures in NTHi: Pneumococcal vaccine impacts on the population genomics of non-typeable Haemophilus influenzae

New Adventures in NTHi: Pneumococcal vaccine impacts on the population genomics of non-typeable Haemophilus influenzae
New Adventures in NTHi: Pneumococcal vaccine impacts on the population genomics of non-typeable Haemophilus influenzae
Background: Non-typeable Haemophilus influenzae (NTHi) are recognised to cause significant human disease. There is evidence that the epidemiology can be altered as a consequence of the introduction of pneumococcal conjugate vaccines (PCVs). Here, we examined the impact of PCV13 introduction in a UK paediatric population.
Methods: Nasopharyngeal swabs were collected from children <5 years of age attending outpatient clinics at University Hospital Southampton Foundation NHS Trust during five consecutive winters, October - March, 2008/9 to 2012/13. The phylogeny of 275 NTHi isolates was examined using hierarchical Bayesian Analysis of Population Structure (hierBAPS). Lineage diversity, stability during PCV13 introduction and levels of recombination were also examined.
Results: NTHi were associated with carriage of vaccine serotype Streptococcus pneumoniae in the pre-PCV13 era (p < 0.05, OR 2.36, 95% CI 1.17-4.75). Following PCV13 introduction, significantly increased carriage of NTHi was observed in two of the three years examined (p < 0.05). Genomic analysis revealed a highly recombinogenic, diverse population (Simpsons MLST diversity, 1-D: 0.97 - 0.99) that could be characterised into eleven temporally stable lineages. Increased carriage was not linked to the expansion of a particular lineage. However a significant association of lineage 6 with S. pneumoniae in both pre- and post-PCV13 eras, OR of 14.75 (95% CI: 3.14-69.38) and 16.95 (95% CI: 0.93-309.96), was observed. 
Conclusion: We have shown that the introduction of PCV13 increased NTHi carriage prevalence in a paediatric population, that the eleven lineages displayed remarkable temporal stability during this period, and there exists lineage specific S. pneumoniae associations.
Cleary, David
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Devine, Vanessa T.
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Morris, Denise
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Osman, Karen
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Bentley, Stephen D.
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Gladstone, Rebecca
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Faust, Saul
f97df780-9f9b-418e-b349-7adf63e150c1
Clarke, Stuart
f7d7f7a2-4b1f-4b36-883a-0f967e73fb17
Cleary, David
f4079c6d-d54b-4108-b346-b0069035bec0
Devine, Vanessa T.
ce4845c0-7013-4e4f-9f57-e1616cc96e98
Morris, Denise
5de4af5f-4112-4bf5-a8b5-6636661244e9
Osman, Karen
10ca3ab1-b308-44f3-9dcd-75a522220bf0
Bentley, Stephen D.
633dc14f-9cef-4fba-b267-83d87a4f45a6
Gladstone, Rebecca
6a2011bf-2561-4956-9928-46e6b927ba6d
Faust, Saul
f97df780-9f9b-418e-b349-7adf63e150c1
Clarke, Stuart
f7d7f7a2-4b1f-4b36-883a-0f967e73fb17

Cleary, David, Devine, Vanessa T., Morris, Denise, Osman, Karen, Bentley, Stephen D., Gladstone, Rebecca, Faust, Saul and Clarke, Stuart (2018) New Adventures in NTHi: Pneumococcal vaccine impacts on the population genomics of non-typeable Haemophilus influenzae. Microbiology Society Annual Conference 2018: Genetics and Genomics Forum, Birmingham, United Kingdom. 09 - 13 Apr 2018.

Record type: Conference or Workshop Item (Poster)

Abstract

Background: Non-typeable Haemophilus influenzae (NTHi) are recognised to cause significant human disease. There is evidence that the epidemiology can be altered as a consequence of the introduction of pneumococcal conjugate vaccines (PCVs). Here, we examined the impact of PCV13 introduction in a UK paediatric population.
Methods: Nasopharyngeal swabs were collected from children <5 years of age attending outpatient clinics at University Hospital Southampton Foundation NHS Trust during five consecutive winters, October - March, 2008/9 to 2012/13. The phylogeny of 275 NTHi isolates was examined using hierarchical Bayesian Analysis of Population Structure (hierBAPS). Lineage diversity, stability during PCV13 introduction and levels of recombination were also examined.
Results: NTHi were associated with carriage of vaccine serotype Streptococcus pneumoniae in the pre-PCV13 era (p < 0.05, OR 2.36, 95% CI 1.17-4.75). Following PCV13 introduction, significantly increased carriage of NTHi was observed in two of the three years examined (p < 0.05). Genomic analysis revealed a highly recombinogenic, diverse population (Simpsons MLST diversity, 1-D: 0.97 - 0.99) that could be characterised into eleven temporally stable lineages. Increased carriage was not linked to the expansion of a particular lineage. However a significant association of lineage 6 with S. pneumoniae in both pre- and post-PCV13 eras, OR of 14.75 (95% CI: 3.14-69.38) and 16.95 (95% CI: 0.93-309.96), was observed. 
Conclusion: We have shown that the introduction of PCV13 increased NTHi carriage prevalence in a paediatric population, that the eleven lineages displayed remarkable temporal stability during this period, and there exists lineage specific S. pneumoniae associations.

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Published date: April 2018
Venue - Dates: Microbiology Society Annual Conference 2018: Genetics and Genomics Forum, Birmingham, United Kingdom, 2018-04-09 - 2018-04-13

Identifiers

Local EPrints ID: 430311
URI: https://eprints.soton.ac.uk/id/eprint/430311
PURE UUID: 3b8d990e-0472-45a7-a809-11854bdb825e
ORCID for David Cleary: ORCID iD orcid.org/0000-0003-4533-0700
ORCID for Saul Faust: ORCID iD orcid.org/0000-0003-3410-7642
ORCID for Stuart Clarke: ORCID iD orcid.org/0000-0002-7009-1548

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Date deposited: 25 Apr 2019 16:30
Last modified: 26 Apr 2019 00:34

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