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Growth kinetics of Chlamydia trachomatis in primary human Sertoli cells

Growth kinetics of Chlamydia trachomatis in primary human Sertoli cells
Growth kinetics of Chlamydia trachomatis in primary human Sertoli cells

Chlamydia trachomatis (Ct) is the leading cause of bacterial sexually transmitted infections worldwide and has been associated with male infertility. Recently, it was hypothesized that Ct may infect the epithelium of the seminiferous tubule, formed by Sertoli cells, thus leading to impaired spermatogenesis. To date, there is a lack of data on Ct infection of the seminiferous epithelium; therefore, we aimed to characterize, for the first time, an in vitro infection model of primary human Sertoli cells. We compared Ct inclusion size, morphology and growth kinetics with those in McCoy cells and we studied F-actin fibres, Vimentin-based intermediate filaments and α-tubulin microtubules in Sertoli and McCoy cells. Our main finding highlighted the ability of Ct to infect Sertoli cells, although with a unique growth profile and the inability to exit host cells. Furthermore, we observed alterations in the cytoskeletal fibres of infected Sertoli cells. Our results suggest that Ct struggles to generate a productive infection in Sertoli cells, limiting its dissemination in the host. Nevertheless, the adverse effect on the cytoskeleton supports the notion that Ct may compromise the blood-testis barrier, impairing spermatogenesis.

2045-2322
1-8
Filardo, Simone
9215c7e9-889d-418f-b519-4d62e700d72f
Skilton, Rachel J.
b02d4f32-609c-4074-b616-ec819b018dbe
O’Neill, Colette E.
d8fc08ba-5ca2-45ab-97ba-bdceb92aa215
Di Pietro, Marisa
2fa23cd4-20d8-460d-88ff-5b34e9f0564f
Sessa, Rosa
d8496f77-38ec-4d9d-9a7a-4db8b740ffa8
Clarke, Ian N.
ff6c9324-3547-4039-bb2c-10c0b3327a8b
Filardo, Simone
9215c7e9-889d-418f-b519-4d62e700d72f
Skilton, Rachel J.
b02d4f32-609c-4074-b616-ec819b018dbe
O’Neill, Colette E.
d8fc08ba-5ca2-45ab-97ba-bdceb92aa215
Di Pietro, Marisa
2fa23cd4-20d8-460d-88ff-5b34e9f0564f
Sessa, Rosa
d8496f77-38ec-4d9d-9a7a-4db8b740ffa8
Clarke, Ian N.
ff6c9324-3547-4039-bb2c-10c0b3327a8b

Filardo, Simone, Skilton, Rachel J., O’Neill, Colette E., Di Pietro, Marisa, Sessa, Rosa and Clarke, Ian N. (2019) Growth kinetics of Chlamydia trachomatis in primary human Sertoli cells. Scientific Reports, 9 (1), 1-8, [5847]. (doi:10.1038/s41598-019-42396-3).

Record type: Article

Abstract

Chlamydia trachomatis (Ct) is the leading cause of bacterial sexually transmitted infections worldwide and has been associated with male infertility. Recently, it was hypothesized that Ct may infect the epithelium of the seminiferous tubule, formed by Sertoli cells, thus leading to impaired spermatogenesis. To date, there is a lack of data on Ct infection of the seminiferous epithelium; therefore, we aimed to characterize, for the first time, an in vitro infection model of primary human Sertoli cells. We compared Ct inclusion size, morphology and growth kinetics with those in McCoy cells and we studied F-actin fibres, Vimentin-based intermediate filaments and α-tubulin microtubules in Sertoli and McCoy cells. Our main finding highlighted the ability of Ct to infect Sertoli cells, although with a unique growth profile and the inability to exit host cells. Furthermore, we observed alterations in the cytoskeletal fibres of infected Sertoli cells. Our results suggest that Ct struggles to generate a productive infection in Sertoli cells, limiting its dissemination in the host. Nevertheless, the adverse effect on the cytoskeleton supports the notion that Ct may compromise the blood-testis barrier, impairing spermatogenesis.

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Accepted/In Press date: 26 March 2019
e-pub ahead of print date: 10 April 2019

Identifiers

Local EPrints ID: 430440
URI: http://eprints.soton.ac.uk/id/eprint/430440
ISSN: 2045-2322
PURE UUID: 15748e03-2bc6-47e4-9948-cc37cb0feaaa
ORCID for Ian N. Clarke: ORCID iD orcid.org/0000-0002-4938-1620

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Date deposited: 01 May 2019 16:30
Last modified: 26 Nov 2021 02:32

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Contributors

Author: Simone Filardo
Author: Rachel J. Skilton
Author: Colette E. O’Neill
Author: Marisa Di Pietro
Author: Rosa Sessa
Author: Ian N. Clarke ORCID iD

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