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The cell in the ink: improving biofabrication by printing stem cells for skeletal regenerative medicine

The cell in the ink: improving biofabrication by printing stem cells for skeletal regenerative medicine
The cell in the ink: improving biofabrication by printing stem cells for skeletal regenerative medicine
Recent advances in regenerative medicine have confirmed the potential to manufacture viable and effective tissue engineering 3D constructs comprising living cells for tissue repair and augmentation. Cell printing has shown promising potential in cell patterning in a number of studies enabling stem cells to be precisely deposited as a blueprint for tissue regeneration guidance. Such manufacturing techniques, however, face a number of challenges including; (i) post-printing cell damage, (ii) proliferation impairment and, (iii) poor or excessive final cell density deposition. The use of hydrogels offers one approach to address these issues given the ability to tune these biomaterials and subsequent application as vectors capable of delivering cell populations and as extrusion pastes. While stem cell-laden hydrogel 3D constructs have been widely established in vitro, clinical relevance, evidenced by in vivo long-term efficacy and clinical application, remains to be demonstrated. This review explores the central features of cell printing, cell-hydrogel properties and cell-biomaterial interactions together with the current advances and challenges in stem cell printing. A key focus is the translational hurdles to clinical application and how in vivo research can reshape and inform cell printing applications for an ageing population.
0142-9612
10-24
Cidonio, Gianluca
558ad583-899a-4d8c-b42b-bc1c354c8757
Glinka, Michael
7630ab6c-91c5-4840-9c25-12cb61fcb91e
Dawson, Jonathan
b220fe76-498d-47be-9995-92da6c289cf3
Oreffo, Richard
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
Cidonio, Gianluca
558ad583-899a-4d8c-b42b-bc1c354c8757
Glinka, Michael
7630ab6c-91c5-4840-9c25-12cb61fcb91e
Dawson, Jonathan
b220fe76-498d-47be-9995-92da6c289cf3
Oreffo, Richard
ff9fff72-6855-4d0f-bfb2-311d0e8f3778

Cidonio, Gianluca, Glinka, Michael, Dawson, Jonathan and Oreffo, Richard (2019) The cell in the ink: improving biofabrication by printing stem cells for skeletal regenerative medicine. Biomaterials, 209, 10-24. (doi:10.1016/j.biomaterials.2019.04.009).

Record type: Article

Abstract

Recent advances in regenerative medicine have confirmed the potential to manufacture viable and effective tissue engineering 3D constructs comprising living cells for tissue repair and augmentation. Cell printing has shown promising potential in cell patterning in a number of studies enabling stem cells to be precisely deposited as a blueprint for tissue regeneration guidance. Such manufacturing techniques, however, face a number of challenges including; (i) post-printing cell damage, (ii) proliferation impairment and, (iii) poor or excessive final cell density deposition. The use of hydrogels offers one approach to address these issues given the ability to tune these biomaterials and subsequent application as vectors capable of delivering cell populations and as extrusion pastes. While stem cell-laden hydrogel 3D constructs have been widely established in vitro, clinical relevance, evidenced by in vivo long-term efficacy and clinical application, remains to be demonstrated. This review explores the central features of cell printing, cell-hydrogel properties and cell-biomaterial interactions together with the current advances and challenges in stem cell printing. A key focus is the translational hurdles to clinical application and how in vivo research can reshape and inform cell printing applications for an ageing population.

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Biomaterials - Cell in the ink - accepted PDF 2019 - Accepted Manuscript
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More information

Accepted/In Press date: 6 April 2019
e-pub ahead of print date: 14 April 2019
Published date: July 2019

Identifiers

Local EPrints ID: 430496
URI: http://eprints.soton.ac.uk/id/eprint/430496
ISSN: 0142-9612
PURE UUID: 28da302e-4acf-42b2-890b-0ad006b39860
ORCID for Jonathan Dawson: ORCID iD orcid.org/0000-0002-6712-0598
ORCID for Richard Oreffo: ORCID iD orcid.org/0000-0001-5995-6726

Catalogue record

Date deposited: 02 May 2019 16:30
Last modified: 07 Oct 2020 04:33

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