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Skin microdialysis: methods, applications and future opportunities - An EAACI position paper

Skin microdialysis: methods, applications and future opportunities - An EAACI position paper
Skin microdialysis: methods, applications and future opportunities - An EAACI position paper

Skin microdialysis (SMD) is a versatile sampling technique that can be used to recover soluble endogenous and exogenous molecules from the extracellular compartment of human skin. Due to its minimally invasive character, SMD can be applied in both clinical and preclinical settings. Despite being available since the 1990s, the technique has still not reached its full potential use as a tool to explore pathophysiological mechanisms of allergic and inflammatory reactions in the skin. Therefore, an EAACI Task Force on SMD was formed to disseminate knowledge about the technique and its many applications. This position paper from the task force provides an overview of the current use of SMD in the investigation of the pathogenesis of chronic inflammatory skin diseases, such as atopic dermatitis, chronic urticaria, psoriasis, and in studies of cutaneous events during type 1 hypersensitivity reactions. Furthermore, this paper covers drug hypersensitivity, UVB-induced- and neurogenic inflammation, and drug penetration investigated by SMD. The aim of this paper is to encourage the use of SMD and to make the technique easily accessible by providing an overview of methodology and applications, supported by standardized operating procedures for SMD in vivo and ex vivo.

Cutaneous, Dermal, Inflammation, Interstitial, Microdialysis
1-13
Baumann, Katrine Y.
bd5c54ee-db57-4be6-b41e-7b620ebf1451
Church, Martin K.
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Clough, Geraldine F.
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Quist, Sven Roy
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Schmelz, Martin
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Skov, Per Stahl
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Anderson, Chris D.
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Tannert, Line Kring
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Giménez-Arnau, Ana Maria
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Frischbutter, Stefan
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Scheffel, Jörg
557a8830-af6a-4fcf-a7ba-f262f91e2d46
Maurer, Marcus
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Baumann, Katrine Y.
bd5c54ee-db57-4be6-b41e-7b620ebf1451
Church, Martin K.
dad189d5-866e-4ae1-b005-0d87f74282b8
Clough, Geraldine F.
9f19639e-a929-4976-ac35-259f9011c494
Quist, Sven Roy
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Schmelz, Martin
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Skov, Per Stahl
1f567cd8-32aa-4757-af93-08d14a878030
Anderson, Chris D.
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Tannert, Line Kring
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Giménez-Arnau, Ana Maria
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Frischbutter, Stefan
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Scheffel, Jörg
557a8830-af6a-4fcf-a7ba-f262f91e2d46
Maurer, Marcus
1a907b66-8e9a-43df-a315-888d407aa4f9

Baumann, Katrine Y., Church, Martin K., Clough, Geraldine F., Quist, Sven Roy, Schmelz, Martin, Skov, Per Stahl, Anderson, Chris D., Tannert, Line Kring, Giménez-Arnau, Ana Maria, Frischbutter, Stefan, Scheffel, Jörg and Maurer, Marcus (2019) Skin microdialysis: methods, applications and future opportunities - An EAACI position paper. Clinical and Translational Allergy, 9 (1), 1-13, [24]. (doi:10.1186/s13601-019-0262-y).

Record type: Review

Abstract

Skin microdialysis (SMD) is a versatile sampling technique that can be used to recover soluble endogenous and exogenous molecules from the extracellular compartment of human skin. Due to its minimally invasive character, SMD can be applied in both clinical and preclinical settings. Despite being available since the 1990s, the technique has still not reached its full potential use as a tool to explore pathophysiological mechanisms of allergic and inflammatory reactions in the skin. Therefore, an EAACI Task Force on SMD was formed to disseminate knowledge about the technique and its many applications. This position paper from the task force provides an overview of the current use of SMD in the investigation of the pathogenesis of chronic inflammatory skin diseases, such as atopic dermatitis, chronic urticaria, psoriasis, and in studies of cutaneous events during type 1 hypersensitivity reactions. Furthermore, this paper covers drug hypersensitivity, UVB-induced- and neurogenic inflammation, and drug penetration investigated by SMD. The aim of this paper is to encourage the use of SMD and to make the technique easily accessible by providing an overview of methodology and applications, supported by standardized operating procedures for SMD in vivo and ex vivo.

Text
s13601-019-0262-y - Version of Record
Available under License Creative Commons Attribution.
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More information

Accepted/In Press date: 25 March 2019
e-pub ahead of print date: 10 April 2019
Keywords: Cutaneous, Dermal, Inflammation, Interstitial, Microdialysis

Identifiers

Local EPrints ID: 430671
URI: http://eprints.soton.ac.uk/id/eprint/430671
PURE UUID: 5d66175b-6c0c-4247-8a35-b9b52c5c6c19
ORCID for Geraldine F. Clough: ORCID iD orcid.org/0000-0002-6226-8964

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Date deposited: 08 May 2019 16:30
Last modified: 07 Oct 2020 01:39

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Contributors

Author: Katrine Y. Baumann
Author: Martin K. Church
Author: Sven Roy Quist
Author: Martin Schmelz
Author: Per Stahl Skov
Author: Chris D. Anderson
Author: Line Kring Tannert
Author: Ana Maria Giménez-Arnau
Author: Stefan Frischbutter
Author: Jörg Scheffel
Author: Marcus Maurer

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