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Hydration and nanomechanical changes in collagen fibrils bearing advanced glycation end-products

Hydration and nanomechanical changes in collagen fibrils bearing advanced glycation end-products
Hydration and nanomechanical changes in collagen fibrils bearing advanced glycation end-products

Accumulation of advanced glycation end-products (AGEs) in biological tissues occurs as a consequence of normal ageing and pathology. Most biological tissues are composed of considerable amounts of collagen, with collagen fibrils being the most abundant form. Collagen fibrils are the smallest discernible structural elements of load-bearing tissues and as such, they are of high biomechanical importance. The low turnover of collagen cause AGEs to accumulate within the collagen fibrils with normal ageing as well as in pathologies. We hypothesized that collagen fibrils bearing AGEs have altered hydration and mechanical properties. To this end, we employed atomic force and Brillouin light scattering microscopy to measure the extent of hydration as well as the transverse elastic properties of collagen fibrils treated with ribose. We find that hydration is different in collagen fibrils bearing AGEs and this is directly related to their mechanical properties. Collagen fibrils treated with ribose showed increased hydration levels and decreased transverse stiffness compared to controlled samples. Our results show that BLS and AFM yield complementary evidence on the effect of hydration on the nanomechanical properties of collagen fibrils.

2156-7085
1841-1855
Andriotis, Orestis G.
d88047ee-bc23-4b1b-9d98-184685fefb65
Elsayad, Kareem
a672c322-73f6-4ed8-942d-89e36f923720
Smart, David E.
3468eafa-5280-40a1-9fda-1bdc07dd914f
Nalbach, Mathis
78a7e3ef-2f34-4c5e-b472-9bce5532881a
Davies, Donna E.
7de8fdc7-3640-4e3a-aa91-d0e03f990c38
Thurner, Philipp J.
ab711ddd-784e-48de-aaad-f56aec40f84f
Andriotis, Orestis G.
d88047ee-bc23-4b1b-9d98-184685fefb65
Elsayad, Kareem
a672c322-73f6-4ed8-942d-89e36f923720
Smart, David E.
3468eafa-5280-40a1-9fda-1bdc07dd914f
Nalbach, Mathis
78a7e3ef-2f34-4c5e-b472-9bce5532881a
Davies, Donna E.
7de8fdc7-3640-4e3a-aa91-d0e03f990c38
Thurner, Philipp J.
ab711ddd-784e-48de-aaad-f56aec40f84f

Andriotis, Orestis G., Elsayad, Kareem, Smart, David E., Nalbach, Mathis, Davies, Donna E. and Thurner, Philipp J. (2019) Hydration and nanomechanical changes in collagen fibrils bearing advanced glycation end-products. Biomedical Optics Express, 10 (4), 1841-1855. (doi:10.1364/BOE.10.001841).

Record type: Article

Abstract

Accumulation of advanced glycation end-products (AGEs) in biological tissues occurs as a consequence of normal ageing and pathology. Most biological tissues are composed of considerable amounts of collagen, with collagen fibrils being the most abundant form. Collagen fibrils are the smallest discernible structural elements of load-bearing tissues and as such, they are of high biomechanical importance. The low turnover of collagen cause AGEs to accumulate within the collagen fibrils with normal ageing as well as in pathologies. We hypothesized that collagen fibrils bearing AGEs have altered hydration and mechanical properties. To this end, we employed atomic force and Brillouin light scattering microscopy to measure the extent of hydration as well as the transverse elastic properties of collagen fibrils treated with ribose. We find that hydration is different in collagen fibrils bearing AGEs and this is directly related to their mechanical properties. Collagen fibrils treated with ribose showed increased hydration levels and decreased transverse stiffness compared to controlled samples. Our results show that BLS and AFM yield complementary evidence on the effect of hydration on the nanomechanical properties of collagen fibrils.

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Accepted/In Press date: 20 February 2019
e-pub ahead of print date: 14 March 2019
Published date: 1 April 2019

Identifiers

Local EPrints ID: 430713
URI: http://eprints.soton.ac.uk/id/eprint/430713
ISSN: 2156-7085
PURE UUID: 967c863d-2eae-4259-858d-bb1279fcf0c1
ORCID for Donna E. Davies: ORCID iD orcid.org/0000-0002-5117-2991
ORCID for Philipp J. Thurner: ORCID iD orcid.org/0000-0001-7588-9041

Catalogue record

Date deposited: 09 May 2019 16:30
Last modified: 07 Oct 2020 01:32

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