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Polycystic ovary syndrome and insulin physiology: An observational quantitative serum proteomics study in adolescent, normal-weight females

Polycystic ovary syndrome and insulin physiology: An observational quantitative serum proteomics study in adolescent, normal-weight females
Polycystic ovary syndrome and insulin physiology: An observational quantitative serum proteomics study in adolescent, normal-weight females

Background: Polycystic ovary syndrome (PCOS) is a common endocrine disorder associated with insulin resistance, even in the absence of overweight/obesity. The aim of the present study is to examine the global serum proteomic profile of adolescent, normal-weight females with PCOS in order to gain novel insight in the association of this endocrine disorder with insulin physiology and to identify novel circulating markers that can guide intervention protocols. Methods: Non-depleted serum from normal-weight (BMI: 18–23 kg m −2 ), adolescent females (13–21 years old) with PCOS (n = 20) is compared to BMI- and age-matched healthy controls (n = 20) using our 3D quantitative proteomics methodology. Serum samples from study participants are randomly pooled to form four biological replicates of females with PCOS and four of healthy controls (n = 5 per sample pool). Results: One-hundred and twenty-six proteins are differentially expressed in females with PCOS compared to controls. Gene ontology analysis shows significant enrichment for terms related to inflammatory immune response, metabolism and insulin-like growth factor receptor signaling pathway. Circulating levels of IGF-1 and -2 and IGFBP-2, -3, and -4 are found to be lower in females with PCOS compared to healthy controls. Conclusions: The present serum proteomics study provides insight into the pro-inflammatory status and insulin dysregulation in young females with PCOS and identifies potential serological markers that can guide early intervention protocols.

inflammation, insulin resistance, iTRAQ, non-depleted serum, PCOS, proteomics
1862-8346
Manousopoulou, Antigoni
9a5e4e75-cea9-4d0b-91c8-0fa2af02632f
Al-Daghri, Nasser M.
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Sabico, Shaun
dd062ab8-a57c-4b27-a35c-b2d0d6b6b0f8
Garay-Baquero, Diana J.
da9136fe-3d47-4d04-8ab3-96bfe17a773c
Teng, Jie
aa0dac71-baf9-499f-9bd4-593e917c9c8c
Alenad, Amal
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Alokail, Majed S.
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Athanasopoulos, Nikos
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Deligeoroglou, Efthymios
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Chrousos, George P.
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Bacopoulou, Flora
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Garbis, Spiros D.
7067fd19-50c9-4d42-9611-f370289470bd
Manousopoulou, Antigoni
9a5e4e75-cea9-4d0b-91c8-0fa2af02632f
Al-Daghri, Nasser M.
0bf1023c-a104-4f74-8b06-87780dfbd8b4
Sabico, Shaun
dd062ab8-a57c-4b27-a35c-b2d0d6b6b0f8
Garay-Baquero, Diana J.
da9136fe-3d47-4d04-8ab3-96bfe17a773c
Teng, Jie
aa0dac71-baf9-499f-9bd4-593e917c9c8c
Alenad, Amal
c29c3870-e904-44c5-bdae-c7ed2bb45b53
Alokail, Majed S.
9adf2d61-d9f7-4095-bc36-701f0680646a
Athanasopoulos, Nikos
b9e70bf4-4bf9-4894-820f-e1f12f0aa8ae
Deligeoroglou, Efthymios
50db9f1c-7e14-43be-8f04-10ddc9852069
Chrousos, George P.
28beffd9-c486-4cf4-a012-06745681d811
Bacopoulou, Flora
838dcaf3-bc25-4d77-8ec9-94887a87324a
Garbis, Spiros D.
7067fd19-50c9-4d42-9611-f370289470bd

Manousopoulou, Antigoni, Al-Daghri, Nasser M., Sabico, Shaun, Garay-Baquero, Diana J., Teng, Jie, Alenad, Amal, Alokail, Majed S., Athanasopoulos, Nikos, Deligeoroglou, Efthymios, Chrousos, George P., Bacopoulou, Flora and Garbis, Spiros D. (2019) Polycystic ovary syndrome and insulin physiology: An observational quantitative serum proteomics study in adolescent, normal-weight females. Proteomics - Clinical Applications, [1800184]. (doi:10.1002/prca.201800184).

Record type: Article

Abstract

Background: Polycystic ovary syndrome (PCOS) is a common endocrine disorder associated with insulin resistance, even in the absence of overweight/obesity. The aim of the present study is to examine the global serum proteomic profile of adolescent, normal-weight females with PCOS in order to gain novel insight in the association of this endocrine disorder with insulin physiology and to identify novel circulating markers that can guide intervention protocols. Methods: Non-depleted serum from normal-weight (BMI: 18–23 kg m −2 ), adolescent females (13–21 years old) with PCOS (n = 20) is compared to BMI- and age-matched healthy controls (n = 20) using our 3D quantitative proteomics methodology. Serum samples from study participants are randomly pooled to form four biological replicates of females with PCOS and four of healthy controls (n = 5 per sample pool). Results: One-hundred and twenty-six proteins are differentially expressed in females with PCOS compared to controls. Gene ontology analysis shows significant enrichment for terms related to inflammatory immune response, metabolism and insulin-like growth factor receptor signaling pathway. Circulating levels of IGF-1 and -2 and IGFBP-2, -3, and -4 are found to be lower in females with PCOS compared to healthy controls. Conclusions: The present serum proteomics study provides insight into the pro-inflammatory status and insulin dysregulation in young females with PCOS and identifies potential serological markers that can guide early intervention protocols.

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Manousopoulou_et_al-2019-PROTEOMICS_-_Clinical_Applications-1 - Version of Record
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Accepted/In Press date: 1 January 2019
e-pub ahead of print date: 9 April 2019
Keywords: inflammation, insulin resistance, iTRAQ, non-depleted serum, PCOS, proteomics

Identifiers

Local EPrints ID: 430795
URI: http://eprints.soton.ac.uk/id/eprint/430795
ISSN: 1862-8346
PURE UUID: 5c47733c-89d0-4fc8-9b89-45d8ea1831b9
ORCID for Diana J. Garay-Baquero: ORCID iD orcid.org/0000-0002-9450-8504
ORCID for Spiros D. Garbis: ORCID iD orcid.org/0000-0002-1050-0805

Catalogue record

Date deposited: 13 May 2019 16:30
Last modified: 26 Nov 2021 03:18

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Contributors

Author: Antigoni Manousopoulou
Author: Nasser M. Al-Daghri
Author: Shaun Sabico
Author: Diana J. Garay-Baquero ORCID iD
Author: Jie Teng
Author: Amal Alenad
Author: Majed S. Alokail
Author: Nikos Athanasopoulos
Author: Efthymios Deligeoroglou
Author: George P. Chrousos
Author: Flora Bacopoulou
Author: Spiros D. Garbis ORCID iD

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