Mid-infrared absorption spectroscopy of protein aggregates using germanium on silicon waveguides
Mid-infrared absorption spectroscopy of protein aggregates using germanium on silicon waveguides
Proteins in human samples can be used to detect the onset of a group of neurodegenerative diseases such as Alzheimer’s and Parkinson’s by studying their conformational (shape and structure) changes that can cause cognitive impairment. Proteins form aggregates from normal state (monomers) to disease state (amyloid deposition and fibril formation in central nervous system) that is associated with disease progression. These changes can be diagnosed and monitored using mid-infrared (MIR) absorption spectroscopy by studying line shapes and relative absorbance of amide bands. We have demonstrated MIR spectroscopy of proteins in three stages of aggregation: monomers, oligomers and fibrils of Bovine Serum Albumin (BSA) protein on a germanium on silicon (GOS) waveguide in the MIR wavelength region of 5.2 – 10 μm (1900 – 1000 cm-1). The protein samples were also characterised by atomic force microscopy to confirm their structure.
European Conference on Integrated Optics
Mittal, Vinita
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Devitt, George
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Bartlett, Philip N.
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Chong, Harold
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Mashanovich, Goran
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Mahajan, Sumeet
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Wilkinson, James S
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24 April 2019
Mittal, Vinita
fd5ee9dd-7770-416f-8f47-50ca158b39b0
Devitt, George
088c46c0-9dcf-4c83-acfd-16c6c9d0ca88
Bartlett, Philip N.
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Chong, Harold
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Mashanovich, Goran
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Mahajan, Sumeet
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Wilkinson, James S
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Mittal, Vinita, Devitt, George, Bartlett, Philip N., Chong, Harold, Mashanovich, Goran, Mahajan, Sumeet and Wilkinson, James S
(2019)
Mid-infrared absorption spectroscopy of protein aggregates using germanium on silicon waveguides.
In Proceedings of the European Conference on Integrated Optics 2019, Gent University, Ghent, Belgium.
European Conference on Integrated Optics..
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Conference or Workshop Item
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Abstract
Proteins in human samples can be used to detect the onset of a group of neurodegenerative diseases such as Alzheimer’s and Parkinson’s by studying their conformational (shape and structure) changes that can cause cognitive impairment. Proteins form aggregates from normal state (monomers) to disease state (amyloid deposition and fibril formation in central nervous system) that is associated with disease progression. These changes can be diagnosed and monitored using mid-infrared (MIR) absorption spectroscopy by studying line shapes and relative absorbance of amide bands. We have demonstrated MIR spectroscopy of proteins in three stages of aggregation: monomers, oligomers and fibrils of Bovine Serum Albumin (BSA) protein on a germanium on silicon (GOS) waveguide in the MIR wavelength region of 5.2 – 10 μm (1900 – 1000 cm-1). The protein samples were also characterised by atomic force microscopy to confirm their structure.
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Mid-infrared absorption spectroscopy of protein aggregates using germanium on silicon waveguides
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Published date: 24 April 2019
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Local EPrints ID: 430845
URI: http://eprints.soton.ac.uk/id/eprint/430845
PURE UUID: 10cd5886-98ac-42c7-ae20-da293aa2a5dd
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Date deposited: 16 May 2019 16:30
Last modified: 23 Feb 2023 03:20
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Author:
Vinita Mittal
Author:
Harold Chong
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