Mid-infrared absorption spectroscopy of protein aggregates using germanium on silicon waveguides
Mid-infrared absorption spectroscopy of protein aggregates using germanium on silicon waveguides
Proteins in human samples can be used to detect the onset of a group of neurodegenerative diseases such as Alzheimer’s and Parkinson’s by studying their conformational (shape and structure) changes that can cause cognitive impairment. Proteins form aggregates from normal state (monomers) to disease state (amyloid deposition and fibril formation in central nervous system) that is associated with disease progression. These changes can be diagnosed and monitored using mid-infrared (MIR) absorption spectroscopy by studying line shapes and relative absorbance of amide bands. We have demonstrated MIR spectroscopy of proteins in three stages of aggregation: monomers, oligomers and fibrils of Bovine Serum Albumin (BSA) protein on a germanium on silicon (GOS) waveguide in the MIR wavelength region of 5.2 – 10 μm (1900 – 1000 cm-1). The protein samples were also characterised by atomic force microscopy to confirm their structure.
European Conference on Integrated Optics
Mittal, Vinita
fd5ee9dd-7770-416f-8f47-50ca158b39b0
Devitt, George
088c46c0-9dcf-4c83-acfd-16c6c9d0ca88
Bartlett, Philip N.
d99446db-a59d-4f89-96eb-f64b5d8bb075
Chong, Harold
795aa67f-29e5-480f-b1bc-9bd5c0d558e1
Mashanovich, Goran
c806e262-af80-4836-b96f-319425060051
Mahajan, Sumeet
b131f40a-479e-4432-b662-19d60d4069e9
Wilkinson, James S
73483cf3-d9f2-4688-9b09-1c84257884ca
24 April 2019
Mittal, Vinita
fd5ee9dd-7770-416f-8f47-50ca158b39b0
Devitt, George
088c46c0-9dcf-4c83-acfd-16c6c9d0ca88
Bartlett, Philip N.
d99446db-a59d-4f89-96eb-f64b5d8bb075
Chong, Harold
795aa67f-29e5-480f-b1bc-9bd5c0d558e1
Mashanovich, Goran
c806e262-af80-4836-b96f-319425060051
Mahajan, Sumeet
b131f40a-479e-4432-b662-19d60d4069e9
Wilkinson, James S
73483cf3-d9f2-4688-9b09-1c84257884ca
Mittal, Vinita, Devitt, George, Bartlett, Philip N., Chong, Harold, Mashanovich, Goran, Mahajan, Sumeet and Wilkinson, James S
(2019)
Mid-infrared absorption spectroscopy of protein aggregates using germanium on silicon waveguides.
In Proceedings of the European Conference on Integrated Optics 2019, Gent University, Ghent, Belgium.
European Conference on Integrated Optics..
Record type:
Conference or Workshop Item
(Paper)
Abstract
Proteins in human samples can be used to detect the onset of a group of neurodegenerative diseases such as Alzheimer’s and Parkinson’s by studying their conformational (shape and structure) changes that can cause cognitive impairment. Proteins form aggregates from normal state (monomers) to disease state (amyloid deposition and fibril formation in central nervous system) that is associated with disease progression. These changes can be diagnosed and monitored using mid-infrared (MIR) absorption spectroscopy by studying line shapes and relative absorbance of amide bands. We have demonstrated MIR spectroscopy of proteins in three stages of aggregation: monomers, oligomers and fibrils of Bovine Serum Albumin (BSA) protein on a germanium on silicon (GOS) waveguide in the MIR wavelength region of 5.2 – 10 μm (1900 – 1000 cm-1). The protein samples were also characterised by atomic force microscopy to confirm their structure.
Text
Mid-infrared absorption spectroscopy of protein aggregates using germanium on silicon waveguides
- Accepted Manuscript
More information
Published date: 24 April 2019
Identifiers
Local EPrints ID: 430845
URI: http://eprints.soton.ac.uk/id/eprint/430845
PURE UUID: 10cd5886-98ac-42c7-ae20-da293aa2a5dd
Catalogue record
Date deposited: 16 May 2019 16:30
Last modified: 16 Mar 2024 04:42
Export record
Contributors
Author:
Vinita Mittal
Author:
Harold Chong
Download statistics
Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.
View more statistics