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Mid-infrared absorption spectroscopy of protein aggregates using germanium on silicon waveguides

Mid-infrared absorption spectroscopy of protein aggregates using germanium on silicon waveguides
Mid-infrared absorption spectroscopy of protein aggregates using germanium on silicon waveguides
Proteins in human samples can be used to detect the onset of a group of neurodegenerative diseases such as Alzheimer’s and Parkinson’s by studying their conformational (shape and structure) changes that can cause cognitive impairment. Proteins form aggregates from normal state (monomers) to disease state (amyloid deposition and fibril formation in central nervous system) that is associated with disease progression. These changes can be diagnosed and monitored using mid-infrared (MIR) absorption spectroscopy by studying line shapes and relative absorbance of amide bands. We have demonstrated MIR spectroscopy of proteins in three stages of aggregation: monomers, oligomers and fibrils of Bovine Serum Albumin (BSA) protein on a germanium on silicon (GOS) waveguide in the MIR wavelength region of 5.2 – 10 μm (1900 – 1000 cm-1). The protein samples were also characterised by atomic force microscopy to confirm their structure.
European Conference on Integrated Optics
Mittal, Vinita
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Devitt, George
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Bartlett, Philip N.
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Chong, Harold
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Mashanovich, Goran
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Mahajan, Sumeet
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Wilkinson, James S
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Mittal, Vinita
fd5ee9dd-7770-416f-8f47-50ca158b39b0
Devitt, George
a27f92f7-fd02-4313-aa62-f274498a43b8
Bartlett, Philip N.
d99446db-a59d-4f89-96eb-f64b5d8bb075
Chong, Harold
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Mashanovich, Goran
c806e262-af80-4836-b96f-319425060051
Mahajan, Sumeet
b131f40a-479e-4432-b662-19d60d4069e9
Wilkinson, James S
73483cf3-d9f2-4688-9b09-1c84257884ca

Mittal, Vinita, Devitt, George, Bartlett, Philip N., Chong, Harold, Mashanovich, Goran, Mahajan, Sumeet and Wilkinson, James S (2019) Mid-infrared absorption spectroscopy of protein aggregates using germanium on silicon waveguides. In Proceedings of the European Conference on Integrated Optics 2019, Gent University, Ghent, Belgium. European Conference on Integrated Optics..

Record type: Conference or Workshop Item (Paper)

Abstract

Proteins in human samples can be used to detect the onset of a group of neurodegenerative diseases such as Alzheimer’s and Parkinson’s by studying their conformational (shape and structure) changes that can cause cognitive impairment. Proteins form aggregates from normal state (monomers) to disease state (amyloid deposition and fibril formation in central nervous system) that is associated with disease progression. These changes can be diagnosed and monitored using mid-infrared (MIR) absorption spectroscopy by studying line shapes and relative absorbance of amide bands. We have demonstrated MIR spectroscopy of proteins in three stages of aggregation: monomers, oligomers and fibrils of Bovine Serum Albumin (BSA) protein on a germanium on silicon (GOS) waveguide in the MIR wavelength region of 5.2 – 10 μm (1900 – 1000 cm-1). The protein samples were also characterised by atomic force microscopy to confirm their structure.

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Mid-infrared absorption spectroscopy of protein aggregates using germanium on silicon waveguides - Accepted Manuscript
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Published date: 24 April 2019

Identifiers

Local EPrints ID: 430845
URI: https://eprints.soton.ac.uk/id/eprint/430845
PURE UUID: 10cd5886-98ac-42c7-ae20-da293aa2a5dd
ORCID for Vinita Mittal: ORCID iD orcid.org/0000-0003-4836-5327
ORCID for Philip N. Bartlett: ORCID iD orcid.org/0000-0002-7300-6900
ORCID for Harold Chong: ORCID iD orcid.org/0000-0002-7110-5761
ORCID for Sumeet Mahajan: ORCID iD orcid.org/0000-0001-8923-6666
ORCID for James S Wilkinson: ORCID iD orcid.org/0000-0003-4712-1697

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Date deposited: 16 May 2019 16:30
Last modified: 17 May 2019 00:39

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