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Isolation and culture of adult ciliary epithelial cells, previously identified as retinal stem cells, and retinal progenitor cells

Isolation and culture of adult ciliary epithelial cells, previously identified as retinal stem cells, and retinal progenitor cells
Isolation and culture of adult ciliary epithelial cells, previously identified as retinal stem cells, and retinal progenitor cells

The protocols described in this unit provide detailed information on how to isolate and expand, in culture, ciliary epithelial cells (CECs), previously identified as retinal stem cells, from the adult mouse eye, and embryonic retinal progenitor cells (RPCs) from the developing retina. CECs are initially cultured in floating conditions as neurospheres and then expanded in monolayer cultures. RPCs are cultured in floating conditions. Detailed protocols for retinal differentiation, as well as exogenous gene expression using lentivirus are also described.

Aging/physiology, Animals, Cell Culture Techniques/methods, Cell Differentiation, Cell Proliferation, Cell Separation/methods, Cells, Cultured, Ciliary Body/cytology, Embryo, Mammalian/cytology, Epithelial Cells/cytology, Lentivirus/genetics, Mice, Retina/cytology, Spheroids, Cellular, Stem Cells/cytology, Transgenes/genetics
1938-8969
1H.4.1-1H.4.15
Gualdoni, Sara
d683d21a-fbc2-4fc3-a292-dc709a96e28a
Baron, Michael
37b594aa-3b71-4ff7-9edf-06e8276ce60c
Lakowski, Jörn
1856e739-982a-412a-87c7-abf1610f5384
Decembrini, Sarah
92766b9c-f219-496d-8159-b21c7552d146
Pearson, Rachel A.
07fad90a-40e9-4a94-80c1-3afa3f18f72e
Ali, Robin R
bddfdcbe-75ce-4264-80cc-c9ef1d6c9d7d
Sowden, Jane C.
e042184a-dbe3-4a58-9f91-c31a5463b893
Gualdoni, Sara
d683d21a-fbc2-4fc3-a292-dc709a96e28a
Baron, Michael
37b594aa-3b71-4ff7-9edf-06e8276ce60c
Lakowski, Jörn
1856e739-982a-412a-87c7-abf1610f5384
Decembrini, Sarah
92766b9c-f219-496d-8159-b21c7552d146
Pearson, Rachel A.
07fad90a-40e9-4a94-80c1-3afa3f18f72e
Ali, Robin R
bddfdcbe-75ce-4264-80cc-c9ef1d6c9d7d
Sowden, Jane C.
e042184a-dbe3-4a58-9f91-c31a5463b893

Gualdoni, Sara, Baron, Michael, Lakowski, Jörn, Decembrini, Sarah, Pearson, Rachel A., Ali, Robin R and Sowden, Jane C. (2011) Isolation and culture of adult ciliary epithelial cells, previously identified as retinal stem cells, and retinal progenitor cells. Current Protocols in Stem Cell Biology, 19 (1), 1H.4.1-1H.4.15. (doi:10.1002/9780470151808.sc01h04s19).

Record type: Article

Abstract

The protocols described in this unit provide detailed information on how to isolate and expand, in culture, ciliary epithelial cells (CECs), previously identified as retinal stem cells, from the adult mouse eye, and embryonic retinal progenitor cells (RPCs) from the developing retina. CECs are initially cultured in floating conditions as neurospheres and then expanded in monolayer cultures. RPCs are cultured in floating conditions. Detailed protocols for retinal differentiation, as well as exogenous gene expression using lentivirus are also described.

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More information

Published date: December 2011
Keywords: Aging/physiology, Animals, Cell Culture Techniques/methods, Cell Differentiation, Cell Proliferation, Cell Separation/methods, Cells, Cultured, Ciliary Body/cytology, Embryo, Mammalian/cytology, Epithelial Cells/cytology, Lentivirus/genetics, Mice, Retina/cytology, Spheroids, Cellular, Stem Cells/cytology, Transgenes/genetics

Identifiers

Local EPrints ID: 431030
URI: http://eprints.soton.ac.uk/id/eprint/431030
ISSN: 1938-8969
PURE UUID: 51c3fe16-b96e-43b5-8f7c-cde9ea901374
ORCID for Jörn Lakowski: ORCID iD orcid.org/0000-0003-4214-7580

Catalogue record

Date deposited: 22 May 2019 16:30
Last modified: 10 Nov 2021 03:50

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Contributors

Author: Sara Gualdoni
Author: Michael Baron
Author: Jörn Lakowski ORCID iD
Author: Sarah Decembrini
Author: Rachel A. Pearson
Author: Robin R Ali
Author: Jane C. Sowden

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