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Late Breaking Abstract - Comparison of the blood transcriptomic profiles of adults and children from the U-BIOPRED asthma study

Late Breaking Abstract - Comparison of the blood transcriptomic profiles of adults and children from the U-BIOPRED asthma study
Late Breaking Abstract - Comparison of the blood transcriptomic profiles of adults and children from the U-BIOPRED asthma study
Background: We have previously reported altered gene expression in adults with asthma compared to healthy controls from the U-BIOPRED study (Bigler, 2016). Altered transcripts may define dysregulated biological pathways and identify novel therapeutic targets. We hypothesised that similar dysregulation would be seen in children with asthma. Aim: To compare blood transcriptomic profiles of children and adults with asthma. Methods: Affymetrix blood transcriptomic profiles of severe asthmatic adult non-smokers, n=152, were compared to mild moderate asthmatics, n=50 (Shaw, 2015; Fleming, 2015). Profiles of school-aged children with severe asthma, n=75, were compared to mild moderate asthmatics, n=37, and in the preschool age group severe wheeze, n=62, was compared to mild moderate wheeze, n=42. Differentially expressed genes (DEG) were identified as probe sets with maximum median group intensity >log2 5, with a significant (raw P≤0.01) change ≥ 20%. Overlapping genes were determined and pathway analysis performed. Results: We found 1887 DEG comparing severe and mild moderate asthmatic adults. Only 28 DEG were found between the severe wheeze and mild pre-school age children, with a larger signature (569 DEG) in the school aged children. 480 genes were specific to school-aged children and 1801 specific to adults, with 89 DEG in common between the adults and school-aged children. Conclusions: Preschool age children were poorly defined in terms of blood transcriptomics by the clinical definitions used. While the school-aged children showed some DEG overlap with the adults, they were distinct in many DEG and pathways indicating that childhood and adult asthma may be mechanistically different.
0903-1936
Affleck, Karen
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Taylor, Adam
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Riley, John H.
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Pavlidis, Stelios
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Fleming, Louise
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Adcock, Ian
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Auffray, Charles
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Bigler, Jeanette
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Bisgaard, Hans
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Boedigheimer, Michael
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Bonnelykke, Klaus
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Bush, Andrew
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Chung, K. Fan
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Djukanovic, Ratko
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Frey, Urs
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Fowler, Stephen
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Hashimoto, Simone
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Bansal, Aruna T.
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Hedlin, Gunila
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Hu, Xuguang
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Murray, Clare
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Nordlund, Bjorn
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Shaw, Dominick
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Singer, Florian
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Sterk, Peter
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Sousa, Ana R.
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Van Aalderen, Wim
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Wagers, Scott
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Yu, Wen
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Roberts, Graham
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Bates, Stewart
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Affleck, Karen
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Taylor, Adam
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Riley, John H.
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Pavlidis, Stelios
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Fleming, Louise
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Adcock, Ian
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Auffray, Charles
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Bigler, Jeanette
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Bisgaard, Hans
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Boedigheimer, Michael
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Bonnelykke, Klaus
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Bush, Andrew
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Chung, K. Fan
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Djukanovic, Ratko
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Frey, Urs
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Fowler, Stephen
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Hashimoto, Simone
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Bansal, Aruna T.
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Hedlin, Gunila
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Hu, Xuguang
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Murray, Clare
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Nordlund, Bjorn
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Shaw, Dominick
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Singer, Florian
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Sterk, Peter
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Sousa, Ana R.
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Van Aalderen, Wim
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Wagers, Scott
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Yu, Wen
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Roberts, Graham
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Bates, Stewart
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Affleck, Karen, Taylor, Adam, Riley, John H., Pavlidis, Stelios, Fleming, Louise, Adcock, Ian, Auffray, Charles, Bigler, Jeanette, Bisgaard, Hans, Boedigheimer, Michael, Bonnelykke, Klaus, Bush, Andrew, Chung, K. Fan, Djukanovic, Ratko, Frey, Urs, Fowler, Stephen, Hashimoto, Simone, Bansal, Aruna T., Hedlin, Gunila, Hu, Xuguang, Murray, Clare, Nordlund, Bjorn, Shaw, Dominick, Singer, Florian, Sterk, Peter, Sousa, Ana R., Van Aalderen, Wim, Wagers, Scott, Yu, Wen, Roberts, Graham and Bates, Stewart (2017) Late Breaking Abstract - Comparison of the blood transcriptomic profiles of adults and children from the U-BIOPRED asthma study. European Respiratory Journal, 50 (Suppl 61), [OA3442]. (doi:10.1183/1393003.congress-2017.OA3442).

Record type: Meeting abstract

Abstract

Background: We have previously reported altered gene expression in adults with asthma compared to healthy controls from the U-BIOPRED study (Bigler, 2016). Altered transcripts may define dysregulated biological pathways and identify novel therapeutic targets. We hypothesised that similar dysregulation would be seen in children with asthma. Aim: To compare blood transcriptomic profiles of children and adults with asthma. Methods: Affymetrix blood transcriptomic profiles of severe asthmatic adult non-smokers, n=152, were compared to mild moderate asthmatics, n=50 (Shaw, 2015; Fleming, 2015). Profiles of school-aged children with severe asthma, n=75, were compared to mild moderate asthmatics, n=37, and in the preschool age group severe wheeze, n=62, was compared to mild moderate wheeze, n=42. Differentially expressed genes (DEG) were identified as probe sets with maximum median group intensity >log2 5, with a significant (raw P≤0.01) change ≥ 20%. Overlapping genes were determined and pathway analysis performed. Results: We found 1887 DEG comparing severe and mild moderate asthmatic adults. Only 28 DEG were found between the severe wheeze and mild pre-school age children, with a larger signature (569 DEG) in the school aged children. 480 genes were specific to school-aged children and 1801 specific to adults, with 89 DEG in common between the adults and school-aged children. Conclusions: Preschool age children were poorly defined in terms of blood transcriptomics by the clinical definitions used. While the school-aged children showed some DEG overlap with the adults, they were distinct in many DEG and pathways indicating that childhood and adult asthma may be mechanistically different.

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e-pub ahead of print date: 6 December 2017
Published date: 2017

Identifiers

Local EPrints ID: 431301
URI: http://eprints.soton.ac.uk/id/eprint/431301
ISSN: 0903-1936
PURE UUID: c937c8c1-6a15-4718-90d9-67680f82f08b
ORCID for Ratko Djukanovic: ORCID iD orcid.org/0000-0001-6039-5612
ORCID for Graham Roberts: ORCID iD orcid.org/0000-0003-2252-1248

Catalogue record

Date deposited: 29 May 2019 16:30
Last modified: 10 Nov 2021 03:13

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Contributors

Author: Karen Affleck
Author: Adam Taylor
Author: John H. Riley
Author: Stelios Pavlidis
Author: Louise Fleming
Author: Ian Adcock
Author: Charles Auffray
Author: Jeanette Bigler
Author: Hans Bisgaard
Author: Michael Boedigheimer
Author: Klaus Bonnelykke
Author: Andrew Bush
Author: K. Fan Chung
Author: Urs Frey
Author: Stephen Fowler
Author: Simone Hashimoto
Author: Aruna T. Bansal
Author: Gunila Hedlin
Author: Xuguang Hu
Author: Clare Murray
Author: Bjorn Nordlund
Author: Dominick Shaw
Author: Florian Singer
Author: Peter Sterk
Author: Ana R. Sousa
Author: Wim Van Aalderen
Author: Scott Wagers
Author: Wen Yu
Author: Graham Roberts ORCID iD
Author: Stewart Bates

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