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Inhibition by the antimicrobial agent chlorhexidine of acid production and sugar transport in oral streptococcal bacteria

Inhibition by the antimicrobial agent chlorhexidine of acid production and sugar transport in oral streptococcal bacteria
Inhibition by the antimicrobial agent chlorhexidine of acid production and sugar transport in oral streptococcal bacteria

Oral streptococci transport sugars via the phosphoenolpyruvate-phosphotransfer-ase (PEP-PTS) system. In a specific assay of this system, low concentrations of chlorhexidine abolished the activity of the glucose and sucrose PTS in batch-grown cells of Streptococcus mutans Irgbritt and B13, Strep. sanguis NCTC 7865, Strep. mitis ATCC 903, Strep. milleri NCTC 10709 and Strep. salivarius NCTC 8606. Intact cells and cells made permeable to the assay reagents with toluene were used. Toluenized cells were more sensitive to chlorhexidine than intact cells (0.09 and 0.25 mM, respectively). This PTS-inhibitory concentration of chlorhexidine reduced acid production from glucose in pH fall experiments to values higher than are obtained solely from endogenous metabolism. The effect of chlorhexidine on rates of acid production was determined at pH 7.0 using cells washed with either 135 mM NaCl or 135 mM KCl. In general, faster rates of acid production from the metabolism of glucose and sucrose were obtained with potassium-treated cells. Addition of the PTS-inhibitory concentration of chlorhexidine markedly reduced or totally abolished acid production by NaCl-treated cells; a greater residual-activity was detected in the same cells washed with KCl (except with Strep. mutans B13 and Strep. mitis ATCC 903). The PTS-inhibitory concentration of chlorhexidine had little or no effect on the viability of cells. The results confirm the existence of sugar uptake systems in oral streptococci additional to the PTS and provide an explanation for the additive anti-caries effect of mouth-rinses containing both fluoride and chlorhexidine.

0003-9969
233-240
Marsh, P. D.
9d226405-bfd2-432b-ac22-ea619f706805
Keevil, C. W.
cb7de0a7-ce33-4cfa-af52-07f99e5650eb
McDermid, A. S.
34686047-11ba-41da-884d-1dc41fa7024b
Williamson, M. I.
73f00143-e678-4e05-8f99-1aa39f569aaf
Ellwood, D. C.
dc74cf9a-6895-42c9-bbd9-46a12236adb1
Marsh, P. D.
9d226405-bfd2-432b-ac22-ea619f706805
Keevil, C. W.
cb7de0a7-ce33-4cfa-af52-07f99e5650eb
McDermid, A. S.
34686047-11ba-41da-884d-1dc41fa7024b
Williamson, M. I.
73f00143-e678-4e05-8f99-1aa39f569aaf
Ellwood, D. C.
dc74cf9a-6895-42c9-bbd9-46a12236adb1

Marsh, P. D., Keevil, C. W., McDermid, A. S., Williamson, M. I. and Ellwood, D. C. (1983) Inhibition by the antimicrobial agent chlorhexidine of acid production and sugar transport in oral streptococcal bacteria. Archives of Oral Biology, 28 (3), 233-240. (doi:10.1016/0003-9969(83)90152-8).

Record type: Article

Abstract

Oral streptococci transport sugars via the phosphoenolpyruvate-phosphotransfer-ase (PEP-PTS) system. In a specific assay of this system, low concentrations of chlorhexidine abolished the activity of the glucose and sucrose PTS in batch-grown cells of Streptococcus mutans Irgbritt and B13, Strep. sanguis NCTC 7865, Strep. mitis ATCC 903, Strep. milleri NCTC 10709 and Strep. salivarius NCTC 8606. Intact cells and cells made permeable to the assay reagents with toluene were used. Toluenized cells were more sensitive to chlorhexidine than intact cells (0.09 and 0.25 mM, respectively). This PTS-inhibitory concentration of chlorhexidine reduced acid production from glucose in pH fall experiments to values higher than are obtained solely from endogenous metabolism. The effect of chlorhexidine on rates of acid production was determined at pH 7.0 using cells washed with either 135 mM NaCl or 135 mM KCl. In general, faster rates of acid production from the metabolism of glucose and sucrose were obtained with potassium-treated cells. Addition of the PTS-inhibitory concentration of chlorhexidine markedly reduced or totally abolished acid production by NaCl-treated cells; a greater residual-activity was detected in the same cells washed with KCl (except with Strep. mutans B13 and Strep. mitis ATCC 903). The PTS-inhibitory concentration of chlorhexidine had little or no effect on the viability of cells. The results confirm the existence of sugar uptake systems in oral streptococci additional to the PTS and provide an explanation for the additive anti-caries effect of mouth-rinses containing both fluoride and chlorhexidine.

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Published date: 1983

Identifiers

Local EPrints ID: 431307
URI: https://eprints.soton.ac.uk/id/eprint/431307
ISSN: 0003-9969
PURE UUID: 8b31debf-c300-4a30-aa38-925a6b910a60
ORCID for C. W. Keevil: ORCID iD orcid.org/0000-0003-1917-7706

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Date deposited: 29 May 2019 16:30
Last modified: 30 May 2019 00:36

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Contributors

Author: P. D. Marsh
Author: C. W. Keevil ORCID iD
Author: A. S. McDermid
Author: M. I. Williamson
Author: D. C. Ellwood

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