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Environmental control of protein export of Bacillus licheniformis NCIB 6346 reveals two types of extracellular proteins

Environmental control of protein export of Bacillus licheniformis NCIB 6346 reveals two types of extracellular proteins
Environmental control of protein export of Bacillus licheniformis NCIB 6346 reveals two types of extracellular proteins

Teichuronic acid was the major anionic polymer of Bacillus licheniformis NCIB 6346 durign phosphate-limited (P-limited) growth in the chemostat. This polymer was also present in significant quantities when B. licheniformis was grown under carbon-limited (C-limited) or magnesium-limited (Mg-limited) conditions where teichoic acid predominated in the cell wall. However, the cell wall composition was not of significance in protein export and the parameters for the excretion process were found to be environmental. In particular, two types of extracellular proteins were identified: the first type of enzyme, penicillinase, was only weakly catabolite repressed; was maximally synthesized and secreted during P-limited growth; was unaffected by growth in high Na+ media but its production was inhibited by gramicidin. The second type of enzyme, α-amylase, was strongly catabolite repressed and its export was markedly inhibited during P-limited growth or in the presence of Na+ or gramicidin. It is noteworthy that the penicillinase carries a glyceride-cysteine modification at its N-terminus whilst the α-amylase does not.

Bacillus licheniformis, Na and gramidicin inhibition, Protein export, Teichoic and teichuronic acids
0302-8933
158-162
Lancaster, M. J.
25b74f91-bbc5-49d3-b18c-42dd7aacfc1c
Keevil, C. W.
cb7de0a7-ce33-4cfa-af52-07f99e5650eb
Ellwood, D. C.
a7dc8f2a-f392-42b4-a022-fa5a58cf6be6
Berkeley, R. C.W.
3e10fba1-a7ba-476d-bc5e-f72d90bc2072
Lancaster, M. J.
25b74f91-bbc5-49d3-b18c-42dd7aacfc1c
Keevil, C. W.
cb7de0a7-ce33-4cfa-af52-07f99e5650eb
Ellwood, D. C.
a7dc8f2a-f392-42b4-a022-fa5a58cf6be6
Berkeley, R. C.W.
3e10fba1-a7ba-476d-bc5e-f72d90bc2072

Lancaster, M. J., Keevil, C. W., Ellwood, D. C. and Berkeley, R. C.W. (1987) Environmental control of protein export of Bacillus licheniformis NCIB 6346 reveals two types of extracellular proteins. Archives of Microbiology, 147 (2), 158-162. (doi:10.1007/BF00415277).

Record type: Article

Abstract

Teichuronic acid was the major anionic polymer of Bacillus licheniformis NCIB 6346 durign phosphate-limited (P-limited) growth in the chemostat. This polymer was also present in significant quantities when B. licheniformis was grown under carbon-limited (C-limited) or magnesium-limited (Mg-limited) conditions where teichoic acid predominated in the cell wall. However, the cell wall composition was not of significance in protein export and the parameters for the excretion process were found to be environmental. In particular, two types of extracellular proteins were identified: the first type of enzyme, penicillinase, was only weakly catabolite repressed; was maximally synthesized and secreted during P-limited growth; was unaffected by growth in high Na+ media but its production was inhibited by gramicidin. The second type of enzyme, α-amylase, was strongly catabolite repressed and its export was markedly inhibited during P-limited growth or in the presence of Na+ or gramicidin. It is noteworthy that the penicillinase carries a glyceride-cysteine modification at its N-terminus whilst the α-amylase does not.

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More information

Published date: 1 March 1987
Keywords: Bacillus licheniformis, Na and gramidicin inhibition, Protein export, Teichoic and teichuronic acids

Identifiers

Local EPrints ID: 431321
URI: http://eprints.soton.ac.uk/id/eprint/431321
ISSN: 0302-8933
PURE UUID: 21e2c90f-e334-4ea9-ad83-d479ae76e172
ORCID for C. W. Keevil: ORCID iD orcid.org/0000-0003-1917-7706

Catalogue record

Date deposited: 29 May 2019 16:30
Last modified: 30 May 2019 00:36

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