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Plasmid stability and antibiotic resistance of Neisseria gonorrhoea during glucose-limited continuous culture

Plasmid stability and antibiotic resistance of Neisseria gonorrhoea during glucose-limited continuous culture
Plasmid stability and antibiotic resistance of Neisseria gonorrhoea during glucose-limited continuous culture

Clinical isolates of Neisseria gonorrhoeae harbouring resistance (R) plasmids of mol. wts 4.4 x 106 (Asian) or 3.2 x 106 (African) were grown in prolonged glucose-limited continuous culture to determine the segregation efficiency of each type of plasmid and their expression of penicillinase activity in the absence of antibiotic selective pressure. One strain contained the African plasmid and cryptic and conjugative plasmids, which were all retained after 96 generations in the chemostat. By contrast, the other strain lost all plasmids after 100 generations. Both strains showed increased sensitivity to a range of antibiotics, particularly to the penicillins. Loss of penicillinase activity as minimal inhibitory concentration decreased was confirmed for both strains by assaying the enzyme spectrophotometrically. Activity decreased with the number of generations and none was detectable at the time of complete plasmid loss. This decrease was apparently due to individual bacteria ceasing to produce enzyme rather than a gradual decline in production by the whole population. The sensitivities to a broad range of antibiotics also generally increased during glucose-limited growth, but one strain became more resistant to clindamycin and the other to tetracycline.

0022-2615
351-357
Keevil, C. W.
cb7de0a7-ce33-4cfa-af52-07f99e5650eb
Spillane, B. J.
705474fa-14b5-49af-b658-33235143b7d3
Major, N. C.
eb96c137-9268-41ab-a1e9-1b2698f7dfea
Keevil, C. W.
cb7de0a7-ce33-4cfa-af52-07f99e5650eb
Spillane, B. J.
705474fa-14b5-49af-b658-33235143b7d3
Major, N. C.
eb96c137-9268-41ab-a1e9-1b2698f7dfea

Keevil, C. W., Spillane, B. J. and Major, N. C. (1987) Plasmid stability and antibiotic resistance of Neisseria gonorrhoea during glucose-limited continuous culture. Journal of Medical Microbiology, 24 (4), 351-357. (doi:10.1099/00222615-24-4-351).

Record type: Article

Abstract

Clinical isolates of Neisseria gonorrhoeae harbouring resistance (R) plasmids of mol. wts 4.4 x 106 (Asian) or 3.2 x 106 (African) were grown in prolonged glucose-limited continuous culture to determine the segregation efficiency of each type of plasmid and their expression of penicillinase activity in the absence of antibiotic selective pressure. One strain contained the African plasmid and cryptic and conjugative plasmids, which were all retained after 96 generations in the chemostat. By contrast, the other strain lost all plasmids after 100 generations. Both strains showed increased sensitivity to a range of antibiotics, particularly to the penicillins. Loss of penicillinase activity as minimal inhibitory concentration decreased was confirmed for both strains by assaying the enzyme spectrophotometrically. Activity decreased with the number of generations and none was detectable at the time of complete plasmid loss. This decrease was apparently due to individual bacteria ceasing to produce enzyme rather than a gradual decline in production by the whole population. The sensitivities to a broad range of antibiotics also generally increased during glucose-limited growth, but one strain became more resistant to clindamycin and the other to tetracycline.

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Published date: 1 December 1987

Identifiers

Local EPrints ID: 431324
URI: http://eprints.soton.ac.uk/id/eprint/431324
ISSN: 0022-2615
PURE UUID: 5867ed0a-981c-4d3f-b223-64d18b8470b2
ORCID for C. W. Keevil: ORCID iD orcid.org/0000-0003-1917-7706

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Date deposited: 29 May 2019 16:30
Last modified: 30 May 2019 00:36

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