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Increased degradative enzyme production by dental plaque bacteria in mucin-limited continuous culture

Increased degradative enzyme production by dental plaque bacteria in mucin-limited continuous culture
Increased degradative enzyme production by dental plaque bacteria in mucin-limited continuous culture

Complex communities of dental plaque bacteria were grown under a variety of continuous culture conditions including one in which hog gastric mucin constituted the principal carbon and nitrogen sources. Washed cells and culture supernatants were assayed for fifteen exoglycosidase activities and for the ability to hydrolyse eleven synthetic peptidase substrates. Under all conditions, cultures elaborated a wide range of exoglycosidases and several peptidase activities that were principally cell-associated. Highest exoglycosidase activities were obtained with cultures growing under conditions in which hog gastric mucin constituted the principal carbon and nitrogen sources. Under these conditions, the principal species isolated were Bacteroides spp. and there was extensive utilisation of protein and carbohydrate moieties of the hog gastric mucin. These data support the hypothesis that supra-gingival plaque bacteria are able in vivo to obtain their nutritional requirements from salivary glycoproteins.

Chemostat, Dental plaque, Glycosidases, Mucin, Proteases
0891-060X
85-94
Beighton, D.
b7845cb5-abb4-421e-9695-78cc6bef1e60
Smith, K.
a2e75daf-e60c-4548-a4f4-0b2394ffc04a
Glenister, D. A.
6992ac98-e915-4ee0-8cd6-c977c9f00d69
Salamon, K.
80ea0367-9180-4e01-977c-c8a34b178a68
Keevil, C. W.
cb7de0a7-ce33-4cfa-af52-07f99e5650eb
Beighton, D.
b7845cb5-abb4-421e-9695-78cc6bef1e60
Smith, K.
a2e75daf-e60c-4548-a4f4-0b2394ffc04a
Glenister, D. A.
6992ac98-e915-4ee0-8cd6-c977c9f00d69
Salamon, K.
80ea0367-9180-4e01-977c-c8a34b178a68
Keevil, C. W.
cb7de0a7-ce33-4cfa-af52-07f99e5650eb

Beighton, D., Smith, K., Glenister, D. A., Salamon, K. and Keevil, C. W. (1988) Increased degradative enzyme production by dental plaque bacteria in mucin-limited continuous culture. Microbial Ecology in Health and Disease, 1 (2), 85-94. (doi:10.3109/08910608809140186).

Record type: Article

Abstract

Complex communities of dental plaque bacteria were grown under a variety of continuous culture conditions including one in which hog gastric mucin constituted the principal carbon and nitrogen sources. Washed cells and culture supernatants were assayed for fifteen exoglycosidase activities and for the ability to hydrolyse eleven synthetic peptidase substrates. Under all conditions, cultures elaborated a wide range of exoglycosidases and several peptidase activities that were principally cell-associated. Highest exoglycosidase activities were obtained with cultures growing under conditions in which hog gastric mucin constituted the principal carbon and nitrogen sources. Under these conditions, the principal species isolated were Bacteroides spp. and there was extensive utilisation of protein and carbohydrate moieties of the hog gastric mucin. These data support the hypothesis that supra-gingival plaque bacteria are able in vivo to obtain their nutritional requirements from salivary glycoproteins.

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More information

Published date: 1988
Keywords: Chemostat, Dental plaque, Glycosidases, Mucin, Proteases

Identifiers

Local EPrints ID: 431326
URI: https://eprints.soton.ac.uk/id/eprint/431326
ISSN: 0891-060X
PURE UUID: 78d82bde-575d-499d-bef2-4eb842ba09b1
ORCID for C. W. Keevil: ORCID iD orcid.org/0000-0003-1917-7706

Catalogue record

Date deposited: 29 May 2019 16:30
Last modified: 30 May 2019 00:36

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