Immunogold and fluorescein immunolabelling of Legionella pneumophila within an aquatic biofilm visualized by using episcopic differential interference contrast microscopy
Immunogold and fluorescein immunolabelling of Legionella pneumophila within an aquatic biofilm visualized by using episcopic differential interference contrast microscopy
Biofilms containing diverse microflora were developed in tap water on glass and polybutylene surfaces. Legionella pneumophila within the biofilms was labelled with monoclonal antibodies and visualized with immunogold or fluorescein isothiocyanate conjugates. Development of a differential interference contrast technique in an episcopic mode enabled simultaneous visualization of the total biofilm flora and gold-labelled legionellae. The legionellae occurred in microcolonies within the biofilm in the absence of amoebae, suggesting that the bacterial consortium was supplying sufficient nutrients to enable legionellae to grow extracellularly within the biofilm.
2326-2330
Rogers, J.
2c2865dd-0bad-4130-b4bf-f9d77c8158c9
Keevil, C. W.
cb7de0a7-ce33-4cfa-af52-07f99e5650eb
1 July 1992
Rogers, J.
2c2865dd-0bad-4130-b4bf-f9d77c8158c9
Keevil, C. W.
cb7de0a7-ce33-4cfa-af52-07f99e5650eb
Rogers, J. and Keevil, C. W.
(1992)
Immunogold and fluorescein immunolabelling of Legionella pneumophila within an aquatic biofilm visualized by using episcopic differential interference contrast microscopy.
Applied and Environmental Microbiology, 58 (7), .
Abstract
Biofilms containing diverse microflora were developed in tap water on glass and polybutylene surfaces. Legionella pneumophila within the biofilms was labelled with monoclonal antibodies and visualized with immunogold or fluorescein isothiocyanate conjugates. Development of a differential interference contrast technique in an episcopic mode enabled simultaneous visualization of the total biofilm flora and gold-labelled legionellae. The legionellae occurred in microcolonies within the biofilm in the absence of amoebae, suggesting that the bacterial consortium was supplying sufficient nutrients to enable legionellae to grow extracellularly within the biofilm.
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Published date: 1 July 1992
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Local EPrints ID: 431332
URI: http://eprints.soton.ac.uk/id/eprint/431332
ISSN: 0099-2240
PURE UUID: 25e1efdf-4514-4376-9de2-bb097de4ff0b
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Date deposited: 29 May 2019 16:30
Last modified: 16 Mar 2024 03:24
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Author:
J. Rogers
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