The University of Southampton
University of Southampton Institutional Repository

Rapid detection of biofilm on corroded copper pipes

Rapid detection of biofilm on corroded copper pipes
Rapid detection of biofilm on corroded copper pipes

Episcopic differential interference contrast (DIC) with UV fluorescence microscopy has been incorporated into a conventional light microscope to view the surface topography of microorganisms, biofilms and opaque substrata surfaces without artefacts. This technique avoids the generation of artefacts produced through preparation for scanning electron microscopy (mainly dehydration) and has been used to investigate microbially influenced corrosion (MIC) in copper and polypropylene pipes from a potable water installation. The copper and polypropylene material examined were obtained from a county hospital in Germany, as well as from test rigs installed in the hospital and in the laboratory where the corrosion process was simulated. Microorganisms such as diatoms and bacteria, were rapidly and reliably detected on and in biofilms on pipe surfaces. The observed biofilm possessed a structure that was neither homogeneous nor confluent over the surface and which ressembled a heterogeneous mosaic of microcolonies.

biofilms, copper corrosion, corrosion, differential interference contrast microscopy, fluorescence microscopy, plumbing tube
0892-7014
55-63
Walker, J. T.
2bb5ed4e-d929-47e4-97ba-70641716acd7
Wagner, D.
5c1a36ac-7706-41d5-9719-c23bc3798888
Fischer, W.
a2ba9f98-f049-4b5e-95a1-8cf82b8cde4c
Keevil, C. W.
cb7de0a7-ce33-4cfa-af52-07f99e5650eb
Walker, J. T.
2bb5ed4e-d929-47e4-97ba-70641716acd7
Wagner, D.
5c1a36ac-7706-41d5-9719-c23bc3798888
Fischer, W.
a2ba9f98-f049-4b5e-95a1-8cf82b8cde4c
Keevil, C. W.
cb7de0a7-ce33-4cfa-af52-07f99e5650eb

Walker, J. T., Wagner, D., Fischer, W. and Keevil, C. W. (1994) Rapid detection of biofilm on corroded copper pipes. Biofouling, 8 (1), 55-63. (doi:10.1080/08927019409378260).

Record type: Article

Abstract

Episcopic differential interference contrast (DIC) with UV fluorescence microscopy has been incorporated into a conventional light microscope to view the surface topography of microorganisms, biofilms and opaque substrata surfaces without artefacts. This technique avoids the generation of artefacts produced through preparation for scanning electron microscopy (mainly dehydration) and has been used to investigate microbially influenced corrosion (MIC) in copper and polypropylene pipes from a potable water installation. The copper and polypropylene material examined were obtained from a county hospital in Germany, as well as from test rigs installed in the hospital and in the laboratory where the corrosion process was simulated. Microorganisms such as diatoms and bacteria, were rapidly and reliably detected on and in biofilms on pipe surfaces. The observed biofilm possessed a structure that was neither homogeneous nor confluent over the surface and which ressembled a heterogeneous mosaic of microcolonies.

Full text not available from this repository.

More information

Accepted/In Press date: 21 September 1993
Published date: 1 April 1994
Keywords: biofilms, copper corrosion, corrosion, differential interference contrast microscopy, fluorescence microscopy, plumbing tube

Identifiers

Local EPrints ID: 431334
URI: https://eprints.soton.ac.uk/id/eprint/431334
ISSN: 0892-7014
PURE UUID: ae746b78-5ae5-4155-b3a2-c07d5e1239e9
ORCID for C. W. Keevil: ORCID iD orcid.org/0000-0003-1917-7706

Catalogue record

Date deposited: 29 May 2019 16:30
Last modified: 30 May 2019 00:36

Export record

Altmetrics

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of https://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×